##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064026_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	661767
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.271255592980612	32.0	32.0	32.0	32.0	32.0
2	31.361300880823613	32.0	32.0	32.0	32.0	32.0
3	31.442231178043027	32.0	32.0	32.0	32.0	32.0
4	31.531103847728883	32.0	32.0	32.0	32.0	32.0
5	31.481722418917837	32.0	32.0	32.0	32.0	32.0
6	35.03958946275653	36.0	36.0	36.0	36.0	36.0
7	35.05067946875562	36.0	36.0	36.0	36.0	36.0
8	35.00998387650034	36.0	36.0	36.0	36.0	36.0
9	35.126973693157865	36.0	36.0	36.0	36.0	36.0
10	34.961341378461	36.0	36.0	36.0	36.0	36.0
11	35.114499514179464	36.0	36.0	36.0	36.0	36.0
12	35.015451057547445	36.0	36.0	36.0	36.0	36.0
13	35.05062506894421	36.0	36.0	36.0	36.0	36.0
14	34.99559512638134	36.0	36.0	36.0	36.0	36.0
15	34.977815454684205	36.0	36.0	36.0	36.0	36.0
16	34.98346245733015	36.0	36.0	36.0	36.0	36.0
17	34.945329700634815	36.0	36.0	36.0	36.0	36.0
18	34.94776258108972	36.0	36.0	36.0	36.0	36.0
19	34.937709193719236	36.0	36.0	36.0	36.0	36.0
20	34.93766083833132	36.0	36.0	36.0	36.0	36.0
21	34.93205463554393	36.0	36.0	36.0	32.0	36.0
22	34.9176235744605	36.0	36.0	36.0	32.0	36.0
23	34.863403584645354	36.0	36.0	36.0	32.0	36.0
24	34.83422110803349	36.0	36.0	36.0	32.0	36.0
25	34.80699853573841	36.0	36.0	36.0	32.0	36.0
26	34.75239321392575	36.0	36.0	36.0	32.0	36.0
27	34.74425288658999	36.0	36.0	36.0	32.0	36.0
28	34.718331376451225	36.0	36.0	36.0	32.0	36.0
29	34.69109973752091	36.0	36.0	36.0	32.0	36.0
30	34.66809617282215	36.0	36.0	36.0	32.0	36.0
31	34.65110832060227	36.0	36.0	36.0	32.0	36.0
32	34.61363591717326	36.0	36.0	36.0	32.0	36.0
33	34.5893418680593	36.0	36.0	36.0	32.0	36.0
34	34.56720114481381	36.0	36.0	36.0	32.0	36.0
35	34.52617462037243	36.0	36.0	36.0	32.0	36.0
36	34.48570267178629	36.0	36.0	36.0	32.0	36.0
37	34.48392863349185	36.0	36.0	36.0	32.0	36.0
38	34.44085153838133	36.0	36.0	36.0	32.0	36.0
39	34.42586439033678	36.0	36.0	36.0	32.0	36.0
40	34.401763762774515	36.0	36.0	36.0	32.0	36.0
41	34.3817536988094	36.0	36.0	36.0	32.0	36.0
42	34.3072833791954	36.0	36.0	36.0	32.0	36.0
43	34.30000287110116	36.0	36.0	36.0	32.0	36.0
44	34.22053230215469	36.0	36.0	36.0	32.0	36.0
45	34.20093628119867	36.0	36.0	36.0	32.0	36.0
46	34.16613097963483	36.0	36.0	36.0	32.0	36.0
47	34.115456044196826	36.0	36.0	36.0	32.0	36.0
48	34.06368404589531	36.0	36.0	36.0	32.0	36.0
49	34.04948418401038	36.0	36.0	36.0	32.0	36.0
50	33.43823581411584	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	4.0
20	7.0
21	26.0
22	70.0
23	234.0
24	571.0
25	1377.0
26	2869.0
27	5124.0
28	8657.0
29	13063.0
30	18360.0
31	25570.0
32	36019.0
33	57019.0
34	115837.0
35	376959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.334396247258965	18.423371210346637	11.810736841150788	26.431495701243605
2	15.714911750483559	20.943091150870405	37.24582930367505	26.096167794970988
3	17.759956721731616	25.036871150440337	29.276297894717757	27.926874233110293
4	11.955869664096276	16.12470854545482	36.71730382445785	35.20211796599105
5	13.847169774255894	37.445505744468974	33.7934650715433	14.913859409731826
6	33.39745468942194	36.58453290135788	16.815007117319414	13.203005291900762
7	29.334040530881715	31.053981235087274	21.474476666258667	18.137501567772343
8	27.72002834834617	33.272737987841644	19.88690883649381	19.120324827318377
9	27.658130868801774	13.777158555492761	18.71080539529317	39.85390518041229
10	15.312569417474995	27.211347972140832	32.59262841193973	24.883454198444444
11	37.113818005430915	20.96236288603088	22.907911697017227	19.015907411520974
12	24.27459252968572	24.04973963606471	29.579791043964182	22.095876790285388
13	29.493595904280074	19.718812144510732	25.54097835482136	25.246613596387835
14	23.2604878461571	19.632014941837447	26.030197985390608	31.077299226614848
15	24.773629840966652	27.703143794918912	22.753223281369948	24.770003082744488
16	25.192439623673625	25.893744918898825	24.35589014848149	24.557925308946064
17	23.51220293547427	26.00703873115462	25.9453856115521	24.53537272181901
18	24.395481182170133	25.168866336439166	26.846800832922817	23.588851648467887
19	25.15945944720725	25.036455429176733	26.100576184669226	23.70350893894679
20	25.48692197612726	24.448779691700455	25.66055013985454	24.40374819231775
21	26.404459575651252	24.385471019256023	25.04567317499966	24.164396230093068
22	25.316161126196985	24.621656867145084	25.619742296004482	24.442439710653446
23	24.137988352378333	24.431747777915454	25.940891493367747	25.489372376338466
24	24.405524661508704	25.126027562862667	25.864815038684718	24.603632736943908
25	24.49040654141349	24.739744285254467	26.01892246805077	24.750926705281277
26	24.296931432491817	25.433930444191237	26.294235517769764	23.97490260554718
27	24.693343085504274	25.140200050473528	25.61909410593094	24.547362758091253
28	24.044536658269703	25.021798361005036	26.303254869308056	24.630410111417202
29	24.17109810517281	25.081866387406706	26.02708882068579	24.719946686734698
30	24.104826193854713	25.33264424406454	26.255207837483734	24.30732172459701
31	24.657559088436194	25.07344496161518	25.41981502750408	24.84918092244454
32	24.307742524344615	25.21624545899648	25.408614759697045	25.067397256961865
33	24.144293628557698	24.82356959140682	25.833492007362473	25.198644772673006
34	24.688516712428957	25.063430010895303	25.954850223572844	24.293203053102893
35	25.246731031064463	24.88572020073955	25.859195198208397	24.00835356998759
36	24.23076516217364	25.43019876716904	25.645543719558912	24.693492351098413
37	24.88262059439994	25.31768375336044	25.492525043710323	24.307170608529294
38	24.298302748758154	25.164685205306803	25.83385344490167	24.703158601033373
39	24.823535278588345	25.104307235242473	25.294107869019427	24.778049617149755
40	25.14083825965014	25.244804700250246	25.665354997038165	23.949002043061448
41	24.215452640041345	25.5635427827083	26.003889680559606	24.21711489669075
42	25.209102434881686	25.624965055148643	25.541852976243547	23.624079533726125
43	24.551949661204432	24.90011424150291	25.822055259038073	24.725880838254582
44	24.19853753405696	25.55658479899782	25.277177945445395	24.96769972149982
45	24.372541582484207	25.75824660713827	25.27317429207398	24.596037518303547
46	24.152104401017898	25.41102762951904	25.412689873609	25.02417809585406
47	24.51847956503737	25.295802997470375	25.668446773593974	24.517270663898284
48	25.10491042757516	25.618846854198306	24.881716042946408	24.394526675280126
49	24.389661571194566	25.802551967166544	25.055911170749695	24.7518752908892
50	24.47530150613288	26.067568200604146	24.77858320451039	24.678547088752588
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	141.0
1	87.0
2	33.0
3	104.5
4	176.0
5	189.5
6	203.0
7	186.5
8	170.0
9	245.0
10	320.0
11	463.5
12	607.0
13	1043.5
14	1480.0
15	1864.0
16	2248.0
17	2428.0
18	2608.0
19	2630.0
20	2652.0
21	2902.5
22	3153.0
23	3568.5
24	3984.0
25	4516.5
26	5049.0
27	6018.0
28	6987.0
29	8147.0
30	9307.0
31	10770.0
32	12233.0
33	13969.5
34	15706.0
35	18164.5
36	20623.0
37	23630.5
38	26638.0
39	28730.5
40	30823.0
41	33466.5
42	36110.0
43	36842.0
44	37574.0
45	40449.0
46	43324.0
47	45663.0
48	48002.0
49	48862.0
50	49722.0
51	47873.5
52	46025.0
53	45568.5
54	45112.0
55	44975.5
56	44839.0
57	43317.0
58	41795.0
59	38104.0
60	34413.0
61	30189.0
62	25965.0
63	22663.0
64	19361.0
65	16498.5
66	13636.0
67	11755.5
68	9875.0
69	8905.0
70	7935.0
71	6262.5
72	4590.0
73	3904.0
74	3218.0
75	2541.0
76	1864.0
77	1574.5
78	1285.0
79	1085.5
80	886.0
81	698.5
82	511.0
83	412.5
84	314.0
85	228.0
86	142.0
87	99.5
88	57.0
89	39.0
90	21.0
91	20.0
92	19.0
93	14.5
94	10.0
95	11.5
96	13.0
97	9.5
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009671077584708818
2	0.0010577741108275269
3	4.533317617832258E-4
4	0.0
5	0.0
6	1.5111058726107528E-4
7	0.0
8	0.0
9	7.555529363053764E-4
10	0.0015111058726107528
11	0.0
12	1.5111058726107528E-4
13	4.533317617832258E-4
14	1.5111058726107528E-4
15	0.00287110115796043
16	1.5111058726107528E-4
17	0.0
18	1.5111058726107528E-4
19	0.0
20	0.0012088846980886022
21	0.0
22	0.0
23	1.5111058726107528E-4
24	0.0010577741108275269
25	0.0021155482216550538
26	0.003777764681526882
27	0.005137759966876559
28	0.0030222117452215055
29	0.0027199905706993548
30	0.0036266540942658065
31	0.007102197601270537
32	0.00287110115796043
33	0.004986649379615484
34	0.0021155482216550538
35	0.0021155482216550538
36	0.00574220231592086
37	0.0036266540942658065
38	0.007555529363053764
39	0.0030222117452215055
40	0.0022666588089161293
41	0.0024177693961772045
42	0.0015111058726107528
43	0.001662216459871828
44	0.0012088846980886022
45	0.0015111058726107528
46	0.001662216459871828
47	0.0013599952853496774
48	0.0018133270471329033
49	4.533317617832258E-4
50	9.066635235664516E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	661767.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.61262763693117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.65958422529573	53.40852936172582
2	11.103232560661912	14.348180620165953
3	2.7898891492326268	5.407862062430472
4	1.1512793327046558	2.97548731320562
5	0.5854600294658494	1.8914055440091841
6	0.36800802267968247	1.4266779202083384
7	0.2463353349481762	1.1141461289577654
8	0.1772251613638338	0.9160786687277146
9	0.1368600430414967	0.7958598299473139
>10	0.6628061134129485	7.8155875141333
>50	0.067308834971927	3.037455318485824
>100	0.049893089370636634	6.114734604173754
>500	0.0021181028505579663	0.7479951138288399
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	1.5111058726107528E-4
10	0.0	0.0	0.0	1.5111058726107528E-4	1.5111058726107528E-4
11	0.0	0.0	0.0	1.5111058726107528E-4	1.5111058726107528E-4
12	0.0	0.0	0.0	3.0222117452215056E-4	4.533317617832258E-4
13	0.0	0.0	0.0	3.0222117452215056E-4	4.533317617832258E-4
14	0.0	0.0	0.0	3.0222117452215056E-4	4.533317617832258E-4
15	0.0	0.0	0.0	3.0222117452215056E-4	4.533317617832258E-4
16	0.0	0.0	0.0	4.533317617832258E-4	6.044423490443011E-4
17	0.0	0.0	0.0	6.044423490443011E-4	6.044423490443011E-4
18	0.0	0.0	0.0	6.044423490443011E-4	6.044423490443011E-4
19	0.0	0.0	0.0	7.555529363053764E-4	6.044423490443011E-4
20	0.0	0.0	0.0	0.0010577741108275269	6.044423490443011E-4
21	0.0	0.0	0.0	0.0012088846980886022	9.066635235664516E-4
22	0.0	0.0	0.0	0.0018133270471329033	9.066635235664516E-4
23	0.0	0.0	0.0	0.0031733223324825807	9.066635235664516E-4
24	0.0	0.0	0.0	0.004382207030571183	9.066635235664516E-4
25	0.0	0.0	0.0	0.004835538792354409	9.066635235664516E-4
26	0.0	0.0	0.0	0.005137759966876559	9.066635235664516E-4
27	0.0	0.0	0.0	0.006951087014009463	9.066635235664516E-4
28	0.0	0.0	0.0	0.009671077584708818	9.066635235664516E-4
29	0.0	0.0	0.0	0.017377717535023655	9.066635235664516E-4
30	0.0	0.0	0.0	0.03007100686495398	9.066635235664516E-4
31	0.0	0.0	0.0	0.058328686682775056	9.066635235664516E-4
32	0.0	0.0	0.0	0.08870191472225118	9.066635235664516E-4
33	0.0	0.0	0.0	0.12330623920503742	9.066635235664516E-4
34	0.0	0.0	0.0	0.1553416837043854	0.0010577741108275269
35	0.0	0.0	0.0	0.1925148881706099	0.0010577741108275269
36	0.0	0.0	0.0	0.24495026195020303	0.0010577741108275269
37	0.0	0.0	0.0	0.32171444027882923	0.0012088846980886022
38	0.0	0.0	0.0	0.43474515955011356	0.0012088846980886022
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCTAA	45	0.0013980356	24.442974	11
TAGGACG	100	1.7425918E-9	24.198544	4
GGCGTTA	190	0.0	23.156504	42
CGCGAGC	50	0.0025801163	21.998676	40
GTATTAG	145	1.2732926E-11	21.241709	1
CGTTATT	225	0.0	19.55438	44
GCGTTAT	215	0.0	19.440691	43
TCTAGAT	205	0.0	19.315912	2
CCGTCGT	160	5.2750693E-11	19.248842	9
GTCCTAA	115	2.017623E-7	19.13073	1
CGGCGTT	230	0.0	19.129284	41
CGTCGTA	150	4.638423E-10	19.06552	10
CCGATCG	185	1.8189894E-12	19.02732	18
CTAGATA	185	1.8189894E-12	19.025885	3
TAAGTAG	105	1.7912189E-6	18.856009	5
TCGCACG	190	1.8189894E-12	18.528002	22
GGGTAAG	95	1.5951175E-5	18.526602	1
GACAACG	60	0.007407985	18.333618	19
CTATATG	60	0.0074112145	18.332232	3
GCGGCGT	240	0.0	18.332232	40
>>END_MODULE