FastQCFastQC Report
Thu 2 Feb 2017
SRR4064025_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064025_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868685
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20450.23541329710999961No Hit
TATCAACGCAGAGTACTTTTTTTTT16490.1898271525351537No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15840.18234457829938355No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11040.12708864548138854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCCG453.5383746E-516.8875585
GCGTTAT402.7695202E-416.616991
CGTTATT456.773941E-414.7732092
ACTGGCG400.005271311414.25216217
GATAACG602.5634103E-514.25133911
TTAGCTC602.5689878E-514.24805454
GATCTAA400.0052935214.2431341
CGCCGGT1152.5465852E-1114.0456087
AGGCCCG1101.8553692E-1013.81868410
ACGAACG551.957979E-413.81868415
ACAATAC1051.369699E-913.5695763
CGCAAGA1351.8189894E-1213.3662372
AACGTCT500.001498206213.3004856
CGAACGA655.442276E-513.15508316
TATAAGG802.0034477E-613.0584622
GATATAC2650.012.8994411
CCGGTCC1403.6379788E-1212.894079
ACGTCTG604.0835649E-412.6685887
CCGATAA604.0855506E-412.6678579
CCCGTTT1051.9861545E-812.66785712
Produced by FastQC (version 0.11.5)