FastQCFastQC Report
Thu 2 Feb 2017
SRR4064025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868685
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC3500.023.259061
GTATTAC1105.2441465E-922.0018141
ATGGGCG904.080357E-721.999285
TTAGTAC808.9871195E-519.249373
ATACCGT1504.656613E-1019.0660446
GACGGTA2100.018.8565258
ATTTCGT1557.203198E-1018.45207242
TCGTTAA1456.2973413E-918.20734812
GTCCTAA2300.018.1754131
TACGACG2200.017.9994135
ACGGTAT2200.017.9994139
CGACGGT2350.017.7866547
ACGACGG2350.017.7866546
GTATAGG1254.8444053E-717.6014521
CAACGTA1254.847334E-717.60043730
CGAAAAC2003.6379788E-1217.60043723
TATTGAG1002.4824069E-517.5994242
CTACGAC2500.017.5994244
CGTCGTA1751.9099389E-1017.59942410
ACTGTTC4400.017.4994288
Produced by FastQC (version 0.11.5)