##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064024_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43236
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30030530113794	32.0	32.0	32.0	32.0	32.0
2	30.984295494495328	32.0	32.0	32.0	32.0	32.0
3	30.977842538625218	32.0	32.0	32.0	32.0	32.0
4	31.02028402257378	32.0	32.0	32.0	32.0	32.0
5	30.98531316495513	32.0	32.0	32.0	32.0	32.0
6	34.62792580257193	36.0	36.0	36.0	32.0	36.0
7	34.56786011656953	36.0	36.0	36.0	32.0	36.0
8	34.566449255250255	36.0	36.0	36.0	32.0	36.0
9	34.670459802016836	36.0	36.0	36.0	32.0	36.0
10	34.44763622906837	36.0	36.0	36.0	32.0	36.0
11	34.55111481173096	36.0	36.0	36.0	32.0	36.0
12	34.508974003145525	36.0	36.0	36.0	32.0	36.0
13	34.53325932093626	36.0	36.0	36.0	32.0	36.0
14	34.419974095661026	36.0	36.0	36.0	32.0	36.0
15	34.39004533259321	36.0	36.0	36.0	32.0	36.0
16	34.416296604681285	36.0	36.0	36.0	32.0	36.0
17	34.321792025164214	36.0	36.0	36.0	32.0	36.0
18	34.339948191322044	36.0	36.0	36.0	32.0	36.0
19	34.33522990100842	36.0	36.0	36.0	32.0	36.0
20	34.31372004810806	36.0	36.0	36.0	32.0	36.0
21	34.339971320196135	36.0	36.0	36.0	32.0	36.0
22	34.31286427976686	36.0	36.0	36.0	32.0	36.0
23	34.23464242760662	36.0	36.0	36.0	32.0	36.0
24	34.23392543250995	36.0	36.0	36.0	32.0	36.0
25	33.87861966879452	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	0.0
6	1.0
7	2.0
8	2.0
9	2.0
10	3.0
11	2.0
12	5.0
13	7.0
14	4.0
15	6.0
16	13.0
17	8.0
18	28.0
19	18.0
20	34.0
21	43.0
22	80.0
23	124.0
24	212.0
25	265.0
26	433.0
27	568.0
28	814.0
29	1041.0
30	1412.0
31	1806.0
32	2550.0
33	3756.0
34	8483.0
35	21513.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.60563152171398	17.39432220448393	12.505495013997825	25.49455125980426
2	17.097259165028333	20.15265409968775	36.90528506996647	25.84480166531745
3	19.061386871443773	23.414442337049543	28.738955451727804	28.78521533977888
4	14.236480547717075	15.406855715409169	35.282416616551785	35.074247120321964
5	16.19096081787482	35.09969005875006	33.362631262432345	15.346717860942777
6	34.63949480696723	35.40283592792209	16.12500289144364	13.832666373667044
7	30.9327719428122	30.23874519964836	21.003562670614908	17.824920186924537
8	27.845260405821243	32.89604590361167	19.41880104578793	19.83989264477916
9	27.172378682340963	15.349547589845649	18.94337352185685	38.53470020595654
10	17.001874696229777	27.321961719165877	32.1730275186891	23.503136065915246
11	36.954660124516856	21.42939801421066	21.98254912398454	19.633392737287938
12	24.576428108508473	23.807980742523842	28.69410239792612	22.921488751041572
13	30.41153596889177	20.16711415609666	24.91898898250162	24.50236089250995
14	24.136574074074073	19.19212962962963	26.002314814814813	30.668981481481485
15	25.633607221386413	26.57331327392663	22.92095822242796	24.872121282258995
16	25.401050950253477	27.067756198060138	23.595453598462925	23.935739253223453
17	23.89065067938242	26.534108006759105	25.372097868104902	24.203143445753568
18	24.473355247928144	25.836844298347145	25.80675031251447	23.88305014121024
19	25.615204759589787	25.49019607843137	26.112924508646433	22.781674653332406
20	26.35197703491064	25.157421983516993	25.435225483841094	23.05537549773127
21	25.575204851627237	25.362251747604276	25.496504791444842	23.56603860932364
22	26.151673688596695	24.459465716005372	25.32987638316589	24.058984212232048
23	25.160901977126453	24.396906977820993	26.010557021808584	24.43163402324397
24	25.16899712936383	24.979164737475692	25.61116770071303	24.24067043244745
25	25.880909385562813	24.427003750520907	25.89248506737047	23.799601796545815
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	3.0
2	7.0
3	11.0
4	11.0
5	11.0
6	38.5
7	66.0
8	66.0
9	66.0
10	87.0
11	108.0
12	108.0
13	108.0
14	116.5
15	125.0
16	125.0
17	125.0
18	221.0
19	317.0
20	317.0
21	317.0
22	495.0
23	673.0
24	673.0
25	673.0
26	956.0
27	1239.0
28	1239.0
29	1239.0
30	1725.5
31	2212.0
32	2212.0
33	2212.0
34	2565.5
35	2919.0
36	2919.0
37	2919.0
38	3370.0
39	3821.0
40	3821.0
41	3821.0
42	4238.5
43	4656.0
44	4656.0
45	4656.0
46	5040.0
47	5424.0
48	5424.0
49	5424.0
50	5485.5
51	5547.0
52	5547.0
53	5547.0
54	5367.0
55	5187.0
56	5187.0
57	5187.0
58	4693.5
59	4200.0
60	4200.0
61	4200.0
62	3578.0
63	2956.0
64	2956.0
65	2956.0
66	2286.5
67	1617.0
68	1617.0
69	1617.0
70	1236.0
71	855.0
72	855.0
73	855.0
74	670.0
75	485.0
76	485.0
77	485.0
78	353.5
79	222.0
80	222.0
81	222.0
82	160.5
83	99.0
84	99.0
85	99.0
86	64.0
87	29.0
88	29.0
89	29.0
90	22.0
91	15.0
92	15.0
93	15.0
94	13.5
95	12.0
96	12.0
97	12.0
98	225.0
99	438.0
100	438.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03469331112961421
2	0.0023128874086409475
3	0.004625774817281895
4	0.004625774817281895
5	0.004625774817281895
6	0.011564437043204737
7	0.023128874086409474
8	0.03469331112961421
9	0.05319641039874179
10	0.06707373485058747
11	0.06707373485058747
12	0.07401239707651032
13	0.07401239707651032
14	0.08326394671107411
15	0.07169950966786937
16	0.08557683411971505
17	0.08095105930243317
18	0.087889721528356
19	0.09020260893699694
20	0.0925154963456379
21	0.0786381718937922
22	0.087889721528356
23	0.09714127116291979
24	0.0925154963456379
25	0.09714127116291979
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	43236.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.59635488944398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.72946465059256	37.200481080581
2	18.27135267674704	20.681839208067352
3	7.51532488761749	12.760199833472107
4	3.6207601144258277	8.196872976223517
5	1.8921127911728646	5.354334351003793
6	1.111565181855333	3.7746322509020263
7	0.670208418471598	2.6551947451198075
8	0.36371066612178177	1.6467758349523545
9	0.2206783816918676	1.1240632805995003
>10	0.5843890478136494	4.609584605421408
>50	0.008173273395995096	0.263669164585068
>100	0.012259910093992642	1.7323526690720694
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	426	0.9852900360810437	No Hit
GTATCAACGCAGAGTACTTTTTTTT	208	0.48108058099731704	No Hit
TATCAACGCAGAGTACTTTTTTTTT	115	0.26598205199370895	No Hit
GGTATCAACGCAGAGTACTTTTTTT	60	0.13877324451845685	No Hit
GTATCAACGCAGAGTACATGGGGGG	54	0.12489592006661115	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	44	0.10176704598020168	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE