FastQCFastQC Report
Thu 2 Feb 2017
SRR4064024_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064024_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43236
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT860.19890831714312146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.12952169488389303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACCT207.772543E-444.00000424
GGTATCA207.772543E-444.0000041
TAGACGT250.002327848735.24
ACCTGCA300.005684688729.33333227
GGCCGTG450.001376536424.4444437
GTATCAA654.827137E-420.3076931
CAACGCA800.001946871416.55
TCAACGC800.001946871416.54
AACGCAG900.004269324214.6666666
ATCAACG1000.00858274413.23
CTTATAC1507.525513E-411.73333444
TCTTATA1600.001292162511.00000143
CTCTTAT1800.0034415689.77777742
TCTCTTA2200.00174996929.041
Produced by FastQC (version 0.11.5)