##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064024_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43236
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24722453510963	32.0	32.0	32.0	32.0	32.0
2	31.48628457766676	32.0	32.0	32.0	32.0	32.0
3	31.551369229345916	32.0	32.0	32.0	32.0	32.0
4	31.597812008511426	32.0	32.0	32.0	32.0	32.0
5	31.590295124433343	32.0	32.0	32.0	32.0	32.0
6	35.18204736793413	36.0	36.0	36.0	36.0	36.0
7	35.166296604681285	36.0	36.0	36.0	36.0	36.0
8	35.1349569802942	36.0	36.0	36.0	36.0	36.0
9	35.21338699232121	36.0	36.0	36.0	36.0	36.0
10	35.08876861874364	36.0	36.0	36.0	36.0	36.0
11	35.17991951151818	36.0	36.0	36.0	36.0	36.0
12	35.14164122490517	36.0	36.0	36.0	36.0	36.0
13	35.11791100009251	36.0	36.0	36.0	36.0	36.0
14	35.097418817651956	36.0	36.0	36.0	36.0	36.0
15	35.07336478860209	36.0	36.0	36.0	36.0	36.0
16	35.125705430659636	36.0	36.0	36.0	36.0	36.0
17	35.075677676010734	36.0	36.0	36.0	36.0	36.0
18	35.05062910537515	36.0	36.0	36.0	36.0	36.0
19	35.06582477564992	36.0	36.0	36.0	36.0	36.0
20	35.06300305301138	36.0	36.0	36.0	36.0	36.0
21	35.04107688037746	36.0	36.0	36.0	36.0	36.0
22	35.052456286427976	36.0	36.0	36.0	36.0	36.0
23	34.98073364788602	36.0	36.0	36.0	36.0	36.0
24	34.984388009991676	36.0	36.0	36.0	36.0	36.0
25	34.94513831066703	36.0	36.0	36.0	36.0	36.0
26	34.904894069756686	36.0	36.0	36.0	36.0	36.0
27	34.87533536867425	36.0	36.0	36.0	32.0	36.0
28	34.8536404847812	36.0	36.0	36.0	32.0	36.0
29	34.84579979646591	36.0	36.0	36.0	32.0	36.0
30	34.85271532981774	36.0	36.0	36.0	32.0	36.0
31	34.79644277916551	36.0	36.0	36.0	32.0	36.0
32	34.80675825700805	36.0	36.0	36.0	32.0	36.0
33	34.747062632991025	36.0	36.0	36.0	32.0	36.0
34	34.75157276343788	36.0	36.0	36.0	32.0	36.0
35	34.68274123415672	36.0	36.0	36.0	32.0	36.0
36	34.65211860486632	36.0	36.0	36.0	32.0	36.0
37	34.582431307243965	36.0	36.0	36.0	32.0	36.0
38	34.575978351373855	36.0	36.0	36.0	32.0	36.0
39	34.47298547506708	36.0	36.0	36.0	32.0	36.0
40	34.4499722453511	36.0	36.0	36.0	32.0	36.0
41	34.40426959015635	36.0	36.0	36.0	32.0	36.0
42	34.47955407530761	36.0	36.0	36.0	32.0	36.0
43	34.39450920529189	36.0	36.0	36.0	32.0	36.0
44	34.35599962993801	36.0	36.0	36.0	32.0	36.0
45	34.35817374410214	36.0	36.0	36.0	32.0	36.0
46	34.26986770284022	36.0	36.0	36.0	32.0	36.0
47	34.181376630585625	36.0	36.0	36.0	32.0	36.0
48	34.22448885188269	36.0	36.0	36.0	32.0	36.0
49	34.18149227495606	36.0	36.0	36.0	32.0	36.0
50	33.53594227033028	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	4.0
23	13.0
24	33.0
25	101.0
26	174.0
27	352.0
28	548.0
29	844.0
30	1115.0
31	1563.0
32	2014.0
33	3105.0
34	6131.0
35	27239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.01614582128565	18.7203627026902	12.673775762762832	26.58971571326132
2	15.94273290776205	20.809048015542604	37.04089184938477	26.20732722731057
3	17.860116569525395	23.885188269035062	29.635026366916456	28.619668794523083
4	12.843463780183182	16.668979554075307	35.75723933758905	34.730317328152466
5	14.497178277361458	36.490424646128226	33.62475714682209	15.387639929688223
6	33.326394671107415	36.06716625034693	16.546396521417336	14.06004255712832
7	30.166990470903876	30.689703025256733	21.132852252752336	18.010454251087058
8	27.187991488574337	33.899990748450364	19.520769728929597	19.391248034045702
9	26.51031547784254	15.55416782311037	18.937922101952076	38.997594597095016
10	16.15551854935702	28.409196040336752	31.97798131186974	23.457304098436488
11	35.31779072994727	22.24072532149135	22.576093995744287	19.865389952817097
12	23.908317143121476	24.107225460264594	28.853270422795816	23.131186973818117
13	29.58876861874364	20.3626607456749	25.404755296512167	24.643815339069295
14	23.644647978536405	19.527708391155517	25.46489036913683	31.362753261171246
15	25.164797039435644	26.899502717705566	22.84029143055395	25.095408812304846
16	24.798778795448236	26.977518734388013	23.51281339624387	24.71088907391988
17	23.26533444351929	27.738458691830882	24.919048940697568	24.07715792395226
18	24.07077599167341	25.680582861107897	25.650514629351218	24.59812651786747
19	24.648441113886577	26.100934406513094	25.846516791562586	23.404107688037744
20	25.765565732260153	25.386252197243035	25.030067536312334	23.818114534184474
21	25.41631973355537	25.716995096678698	24.993061337774076	23.873623831991857
22	25.168840780830788	25.69155333518364	24.817281894717365	24.322323989268202
23	25.02544176149505	24.694698862059393	25.09945415857156	25.18040521787399
24	25.1410861319271	25.494957905449162	24.937552039966697	24.426403922657045
25	24.713773563085464	25.63894992482942	25.82629813808257	23.820978374002543
26	24.537947306331105	24.984386204344105	26.372279151534777	24.105387337790013
27	24.070690014110244	26.654484050796885	25.060720316439593	24.21410561865328
28	24.52179946802359	24.94044177171273	26.027523996761882	24.510234763501792
29	24.2789406730658	26.441540418642305	24.739215913033423	24.54030299525847
30	24.584817504741636	25.556275153814127	25.359670629597076	24.499236711847157
31	24.487105354458194	25.227246443853357	25.955822828726728	24.32982537296172
32	24.078828672541807	25.23766567205607	25.980154048990357	24.70335160641177
33	24.510189447387294	25.04915454187967	26.254308251023573	24.186347759709466
34	24.812064857863206	25.091943654153077	25.607753336571598	24.488238151412116
35	24.484792413553834	24.905747658147334	27.39215913033422	23.21730079796461
36	24.407846039970394	25.534326424870468	25.737879348630642	24.3199481865285
37	24.148220109638473	25.950084426248466	25.663266486248933	24.23842897786413
38	24.321698702380125	24.664029792056994	26.338676473989775	24.675595031573103
39	23.752688918187495	24.68253417528277	26.13281521060301	25.431961695926724
40	23.966693650977216	25.39377818896727	26.275008673528394	24.36451948652712
41	23.729009575796827	24.63107739279271	26.539297774899385	25.100615256511077
42	23.805338391081094	24.42290789656289	26.613313595781097	25.15844011657492
43	23.640022203719123	24.523545193819967	27.014524932926264	24.821907669534646
44	23.304653529466187	24.43796835970025	26.438615968174666	25.818762142658898
45	23.08955500046258	24.6044962531224	27.65750763252845	24.648441113886577
46	24.18817651956703	23.952262003885654	26.295216948838927	25.564344527708393
47	22.6424259246409	24.497490343025007	27.592348437536142	25.267735294797955
48	23.10450108710737	24.335014109265856	26.99033168339733	25.57015312022945
49	23.355537052456288	24.208992506244794	27.16717550189657	25.268294939402352
50	23.08955500046258	24.53742251827181	27.743084466648167	24.629938014617448
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	4.0
4	8.0
5	9.5
6	11.0
7	13.5
8	16.0
9	25.5
10	35.0
11	49.5
12	64.0
13	95.0
14	126.0
15	184.0
16	242.0
17	217.0
18	192.0
19	162.5
20	133.0
21	142.5
22	152.0
23	157.0
24	162.0
25	187.0
26	212.0
27	262.0
28	312.0
29	395.5
30	479.0
31	597.0
32	715.0
33	944.5
34	1174.0
35	1262.5
36	1351.0
37	1529.0
38	1707.0
39	1888.0
40	2069.0
41	2254.5
42	2440.0
43	2497.0
44	2554.0
45	2796.5
46	3039.0
47	3142.5
48	3246.0
49	3244.5
50	3243.0
51	3416.0
52	3589.0
53	3376.0
54	3163.0
55	3119.0
56	3075.0
57	2861.0
58	2647.0
59	2301.0
60	1955.0
61	1808.0
62	1661.0
63	1389.0
64	1117.0
65	1003.0
66	889.0
67	731.5
68	574.0
69	413.0
70	252.0
71	215.5
72	179.0
73	158.5
74	138.0
75	115.0
76	92.0
77	87.5
78	83.0
79	72.5
80	62.0
81	51.0
82	40.0
83	28.0
84	16.0
85	15.5
86	15.0
87	9.5
88	4.0
89	2.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011564437043204737
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0023128874086409475
16	0.0
17	0.0
18	0.0023128874086409475
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0023128874086409475
26	0.011564437043204737
27	0.011564437043204737
28	0.0023128874086409475
29	0.0023128874086409475
30	0.004625774817281895
31	0.0023128874086409475
32	0.006938662225922843
33	0.011564437043204737
34	0.006938662225922843
35	0.0023128874086409475
36	0.00925154963456379
37	0.006938662225922843
38	0.006938662225922843
39	0.006938662225922843
40	0.0023128874086409475
41	0.004625774817281895
42	0.004625774817281895
43	0.0
44	0.0
45	0.0
46	0.0
47	0.006938662225922843
48	0.004625774817281895
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	43236.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.18262558978628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.55855855855856	46.56998797298548
2	15.102702702702702	19.38662225922842
3	6.162162162162162	11.865112406328059
4	2.774774774774775	7.123693218614117
5	1.4486486486486487	4.648903691368305
6	0.8108108108108109	3.122398001665279
7	0.3963963963963964	1.7809233046535293
8	0.23783783783783785	1.2212045517624202
9	0.16216216216216214	0.9367194004995838
>10	0.3387387387387387	3.0160051808677957
>50	0.007207207207207207	0.32843001202701455
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	86	0.19890831714312146	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56	0.12952169488389303	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0023128874086409475	0.0
17	0.0	0.0	0.0	0.0023128874086409475	0.0
18	0.0	0.0	0.0	0.0023128874086409475	0.0
19	0.0	0.0	0.0	0.0023128874086409475	0.0
20	0.0	0.0	0.0	0.0023128874086409475	0.0
21	0.0	0.0	0.0	0.0023128874086409475	0.0
22	0.0	0.0	0.0	0.0023128874086409475	0.0
23	0.0	0.0	0.0	0.0023128874086409475	0.0
24	0.0	0.0	0.0	0.0023128874086409475	0.0
25	0.0	0.0	0.0	0.0023128874086409475	0.0
26	0.0	0.0	0.0	0.0023128874086409475	0.0
27	0.0	0.0	0.0	0.0023128874086409475	0.0
28	0.0	0.0	0.0	0.023128874086409474	0.0
29	0.0	0.0	0.0	0.07169950966786937	0.0
30	0.0	0.0	0.0	0.13877324451845685	0.0
31	0.0	0.0	0.0	0.2983624757146822	0.0
32	0.0	0.0	0.0	0.4926450180405218	0.0
33	0.0	0.0	0.0	0.7169950966786937	0.0
34	0.0	0.0	0.0	0.8858358775094828	0.0
35	0.0	0.0	0.0	1.103247293921732	0.0
36	0.0	0.0	0.0	1.4339901933573873	0.0
37	0.0	0.0	0.0	1.8549357017300399	0.0
38	0.0	0.0	0.0	2.426218891664354	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACCT	20	7.772543E-4	44.000004	24
GGTATCA	20	7.772543E-4	44.000004	1
TAGACGT	25	0.0023278487	35.2	4
ACCTGCA	30	0.0056846887	29.333332	27
GGCCGTG	45	0.0013765364	24.444443	7
GTATCAA	65	4.827137E-4	20.307693	1
CAACGCA	80	0.0019468714	16.5	5
TCAACGC	80	0.0019468714	16.5	4
AACGCAG	90	0.0042693242	14.666666	6
ATCAACG	100	0.008582744	13.2	3
CTTATAC	150	7.525513E-4	11.733334	44
TCTTATA	160	0.0012921625	11.000001	43
CTCTTAT	180	0.003441568	9.777777	42
TCTCTTA	220	0.0017499692	9.0	41
>>END_MODULE