FastQCFastQC Report
Thu 2 Feb 2017
SRR4064023_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064023_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766726
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34940.19776694292153962No Hit
TATCAACGCAGAGTACTTTTTTTTT22720.12859945458435548No Hit
GCGCAAGACGGACCAGAGCGAAAGC20460.11580743137305953No Hit
GATTAAGAGGGACGGCCGGGGGCAT20300.11490180141119788No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20210.1143923845576507No Hit
GTATCTGATCGTCTTCGAACCTCCG19560.11071326283758771No Hit
GAACTACGACGGTATCTGATCGTCT19160.10844918793293358No Hit
GAATAGGACCGCGGTTCTATTTTGT19020.10765676171630462No Hit
ATCAGATACCGTCGTAGTTCCGACC18900.10697753924490838No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18090.10239278756298373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG250.00603073319.00090811
GCGTTAT1650.013.8145361
CGTTATT1650.013.2348062
GTATCAA19500.012.8581441
TTATACC604.1035976E-412.6626064
TTAGAGT2650.012.5431494
CCGCGCG701.09144195E-412.213839
AAACCGA701.09201945E-412.2131386
CCTATAC701.09577966E-412.2086423
GATCGTT550.003069587312.0904577
ATAGTAG658.055541E-411.6869063
CTAGCCT907.488732E-611.6073894
CGACCAT3850.011.597310
ACTCTAA2550.011.54891710
AACCGCG2000.011.3995757
TAGGAGT752.0803032E-411.3963464
TATACTG1354.7402864E-911.2575645
ATTATAC855.348695E-511.1713073
CGAACGA2050.011.12500416
GCTCGTA2150.011.04619
Produced by FastQC (version 0.11.5)