##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064023_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1766726 Sequences flagged as poor quality 0 Sequence length 25 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.301689113082617 32.0 32.0 32.0 32.0 32.0 2 30.912379169152434 32.0 32.0 32.0 32.0 32.0 3 30.925700985891417 32.0 32.0 32.0 32.0 32.0 4 30.954571337038114 32.0 32.0 32.0 32.0 32.0 5 30.85950849197895 32.0 32.0 32.0 32.0 32.0 6 34.52664476551542 36.0 36.0 36.0 32.0 36.0 7 34.476226647482406 36.0 36.0 36.0 32.0 36.0 8 34.443678872671825 36.0 36.0 36.0 32.0 36.0 9 34.57459504190236 36.0 36.0 36.0 32.0 36.0 10 34.28018436361948 36.0 36.0 36.0 32.0 36.0 11 34.53381848685082 36.0 36.0 36.0 32.0 36.0 12 34.365236035468996 36.0 36.0 36.0 32.0 36.0 13 34.44493713230008 36.0 36.0 36.0 32.0 36.0 14 34.33820128305125 36.0 36.0 36.0 32.0 36.0 15 34.27733162923962 36.0 36.0 36.0 32.0 36.0 16 34.2779412314077 36.0 36.0 36.0 32.0 36.0 17 34.18805915574911 36.0 36.0 36.0 32.0 36.0 18 34.19067472828271 36.0 36.0 36.0 32.0 36.0 19 34.209182408590806 36.0 36.0 36.0 32.0 36.0 20 34.18952514424987 36.0 36.0 36.0 32.0 36.0 21 34.173014944026406 36.0 36.0 36.0 32.0 36.0 22 34.15960935651595 36.0 36.0 36.0 32.0 36.0 23 34.10484025253491 36.0 36.0 36.0 32.0 36.0 24 34.08626351794223 36.0 36.0 36.0 32.0 36.0 25 33.6873018226935 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 9.0 5 28.0 6 78.0 7 19.0 8 85.0 9 111.0 10 152.0 11 43.0 12 113.0 13 87.0 14 157.0 15 275.0 16 476.0 17 608.0 18 831.0 19 1055.0 20 1586.0 21 2320.0 22 3606.0 23 5631.0 24 8657.0 25 12775.0 26 17757.0 27 23923.0 28 33441.0 29 45704.0 30 60918.0 31 83328.0 32 118860.0 33 170385.0 34 368256.0 35 805448.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.77718991193526 16.6913634492525 10.914071617083417 26.61737502172883 2 17.66882500479725 19.360966627552802 35.861300312401475 27.108908055248477 3 19.11040772696167 23.048377848606748 28.045782610147636 29.795431814283944 4 13.334597489047129 14.82939558262484 34.66111192872426 37.17489499960377 5 15.564603869880889 35.45590711017873 32.85277764883961 16.126711371100768 6 35.16209179852352 34.30587846355984 16.39751848147098 14.13451125644566 7 31.13077013659915 29.576082919671006 20.50232798289793 18.790818960831913 8 28.768600095123777 31.63426041265599 19.257071999637624 20.340067492582612 9 27.699464846957554 13.85315853554945 18.143613557210408 40.303763060282584 10 16.67698357996703 25.56202635881677 30.53968689064846 27.221303170567744 11 38.092152917277126 20.6053102978413 21.794840573833394 19.507696211048177 12 25.495932012358303 22.921474953486463 27.75678729507848 23.825805739076753 13 29.752948459517338 18.633391916437798 24.898687855512883 26.71497176853198 14 24.356477141748627 18.930308033451237 24.069371953861534 32.6438428709386 15 25.692160526305358 26.258575371233643 21.582665361022208 26.46659874143879 16 26.70780962996821 24.962801980798062 22.70050539463318 25.628882994600556 17 24.97769924134943 25.084799519719535 24.266057254189004 25.67144398474203 18 25.94655955390579 24.015799547629474 24.97429712915218 25.063343769312556 19 26.31318984453681 24.092659368744457 24.527056803029737 25.067093983689 20 26.54679674037787 23.406187300252977 23.902857097540554 26.144158861828593 21 27.641950832672478 23.360654809108418 23.634133907329783 25.363260450889317 22 27.007084677960684 23.13399144310024 23.99472288639308 25.864200992545992 23 25.335956557058516 23.104158145721478 24.66172083627436 26.89816446094565 24 25.616033380028064 24.056867495540587 24.264642989248127 26.062456135183222 25 25.671714330868696 23.522874228703202 24.32298933198744 26.482422108440662 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 85.0 1 85.0 2 236.0 3 387.0 4 387.0 5 387.0 6 1131.5 7 1876.0 8 1876.0 9 1876.0 10 2053.0 11 2230.0 12 2230.0 13 2230.0 14 2671.5 15 3113.0 16 3113.0 17 3113.0 18 5445.0 19 7777.0 20 7777.0 21 7777.0 22 12810.5 23 17844.0 24 17844.0 25 17844.0 26 27587.5 27 37331.0 28 37331.0 29 37331.0 30 47424.5 31 57518.0 32 57518.0 33 57518.0 34 75446.0 35 93374.0 36 93374.0 37 93374.0 38 111222.0 39 129070.0 40 129070.0 41 129070.0 42 152777.0 43 176484.0 44 176484.0 45 176484.0 46 200350.0 47 224216.0 48 224216.0 49 224216.0 50 234785.0 51 245354.0 52 245354.0 53 245354.0 54 232242.0 55 219130.0 56 219130.0 57 219130.0 58 205429.5 59 191729.0 60 191729.0 61 191729.0 62 170482.5 63 149236.0 64 149236.0 65 149236.0 66 123562.5 67 97889.0 68 97889.0 69 97889.0 70 74969.0 71 52049.0 72 52049.0 73 52049.0 74 40212.5 75 28376.0 76 28376.0 77 28376.0 78 23187.0 79 17998.0 80 17998.0 81 17998.0 82 12440.5 83 6883.0 84 6883.0 85 6883.0 86 5274.5 87 3666.0 88 3666.0 89 3666.0 90 2618.0 91 1570.0 92 1570.0 93 1570.0 94 1053.5 95 537.0 96 537.0 97 537.0 98 770.5 99 1004.0 100 1004.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03616859660184998 2 0.005037566662855474 3 0.0016414543058742557 4 0.0037357235926793403 5 0.009056299618616582 6 0.012848625083912275 7 0.02207473032037792 8 0.034300734805510304 9 0.049583240411925786 10 0.057564104450831655 11 0.05586604827234104 12 0.0628846804767689 13 0.0646959404004922 14 0.06628079283375012 15 0.05994138310071851 16 0.06650720032421553 17 0.06175264302444182 18 0.0769219448856246 19 0.0731296194203289 20 0.07743136173917178 21 0.07505408308928492 22 0.07737475986655543 23 0.08309154900080715 24 0.0769219448856246 25 0.07669553739515919 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1766726.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.408445301079304 #Duplication Level Percentage of deduplicated Percentage of total 1 76.24591697926229 37.671922185005144 2 13.418803053941017 13.260043933932012 3 4.259317361702649 6.313387466568683 4 1.9315347636079092 3.817365188594181 5 1.0320091854664324 2.5494984695164815 6 0.6699537802437452 1.9860824823254635 7 0.43759766902728553 1.5134714345810112 8 0.33325981760517814 1.3172679575354487 9 0.25670694330489163 1.1415141870018302 >10 1.2401033103309933 10.683895940164055 >50 0.07671040367298235 2.621930594189698 >100 0.08003368910066835 8.859975320587628 >500 0.012648628667133744 4.332885582339752 >1k 0.005404414066866236 3.9307592576586954 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3494 0.19776694292153962 No Hit TATCAACGCAGAGTACTTTTTTTTT 2272 0.12859945458435548 No Hit GCGCAAGACGGACCAGAGCGAAAGC 2046 0.11580743137305953 No Hit GATTAAGAGGGACGGCCGGGGGCAT 2030 0.11490180141119788 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 2021 0.1143923845576507 No Hit GTATCTGATCGTCTTCGAACCTCCG 1956 0.11071326283758771 No Hit GAACTACGACGGTATCTGATCGTCT 1916 0.10844918793293358 No Hit GAATAGGACCGCGGTTCTATTTTGT 1902 0.10765676171630462 No Hit ATCAGATACCGTCGTAGTTCCGACC 1890 0.10697753924490838 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 1809 0.10239278756298373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.660187261635364E-5 2 0.0 0.0 0.0 0.0 5.660187261635364E-5 3 0.0 0.0 0.0 0.0 5.660187261635364E-5 4 0.0 0.0 0.0 0.0 5.660187261635364E-5 5 0.0 0.0 0.0 0.0 1.1320374523270728E-4 6 0.0 0.0 0.0 0.0 1.6980561784906093E-4 7 0.0 0.0 0.0 0.0 1.6980561784906093E-4 8 0.0 0.0 0.0 0.0 1.6980561784906093E-4 9 0.0 0.0 0.0 0.0 1.6980561784906093E-4 10 0.0 0.0 0.0 0.0 1.6980561784906093E-4 11 0.0 0.0 0.0 0.0 1.6980561784906093E-4 12 0.0 0.0 0.0 0.0 2.830093630817682E-4 13 0.0 0.0 0.0 0.0 3.3961123569812185E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCCCG 25 0.006030733 19.000908 11 GCGTTAT 165 0.0 13.814536 1 CGTTATT 165 0.0 13.234806 2 GTATCAA 1950 0.0 12.858144 1 TTATACC 60 4.1035976E-4 12.662606 4 TTAGAGT 265 0.0 12.543149 4 CCGCGCG 70 1.09144195E-4 12.21383 9 AAACCGA 70 1.09201945E-4 12.213138 6 CCTATAC 70 1.09577966E-4 12.208642 3 GATCGTT 55 0.0030695873 12.090457 7 ATAGTAG 65 8.055541E-4 11.686906 3 CTAGCCT 90 7.488732E-6 11.607389 4 CGACCAT 385 0.0 11.5973 10 ACTCTAA 255 0.0 11.548917 10 AACCGCG 200 0.0 11.399575 7 TAGGAGT 75 2.0803032E-4 11.396346 4 TATACTG 135 4.7402864E-9 11.257564 5 ATTATAC 85 5.348695E-5 11.171307 3 CGAACGA 205 0.0 11.125004 16 GCTCGTA 215 0.0 11.0461 9 >>END_MODULE