FastQCFastQC Report
Thu 2 Feb 2017
SRR4064023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766726
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA21070.11926014560265713No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20440.11569422762782684No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19140.10833598418770088No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG19040.10776996546153733No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18660.10561909430211588No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA18550.104996473703336No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT18030.1020531763272856No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC17820.10086453700234219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23850.025.7339951
GTATTAC1351.1459633E-1021.183661
GTTCGCG755.470347E-520.53534138
GGTATCA10450.020.419581
TATCGTT654.937684E-420.3050817
TACCGTA654.937684E-420.305087
TATACTG2750.018.3976335
ACCGTCG4900.017.9568738
GTACCGT1601.1041266E-917.87276
AACGCAG34250.017.7904116
ATACCGT5500.017.1977886
TCAACGC35850.017.1804964
TTAACGG1551.438093E-817.03440735
ATCAACG36250.016.9909173
GTAAACG5200.016.9256927
CAACGCA36750.016.759755
TATCAAC37200.016.7344062
TACCGTC5400.016.7015557
CGTCGTA5450.016.54926710
TAAACGC5350.016.45067428
Produced by FastQC (version 0.11.5)