##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064023_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1766726
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.348543577215708	32.0	32.0	32.0	32.0	32.0
2	31.431403624557515	32.0	32.0	32.0	32.0	32.0
3	31.489823549322306	32.0	32.0	32.0	32.0	32.0
4	31.56896428761449	32.0	32.0	32.0	32.0	32.0
5	31.515168735842458	32.0	32.0	32.0	32.0	32.0
6	35.1083988122663	36.0	36.0	36.0	36.0	36.0
7	35.127407419147055	36.0	36.0	36.0	36.0	36.0
8	35.07155608736159	36.0	36.0	36.0	36.0	36.0
9	35.18231236762237	36.0	36.0	36.0	36.0	36.0
10	35.02818829858167	36.0	36.0	36.0	36.0	36.0
11	35.168536603865	36.0	36.0	36.0	36.0	36.0
12	35.074464291576625	36.0	36.0	36.0	36.0	36.0
13	35.11474671228023	36.0	36.0	36.0	36.0	36.0
14	35.05606019269541	36.0	36.0	36.0	36.0	36.0
15	35.03181421454148	36.0	36.0	36.0	36.0	36.0
16	35.0389539747533	36.0	36.0	36.0	36.0	36.0
17	34.99685067180763	36.0	36.0	36.0	36.0	36.0
18	34.99320324713623	36.0	36.0	36.0	36.0	36.0
19	34.99315796563813	36.0	36.0	36.0	36.0	36.0
20	34.9885103858776	36.0	36.0	36.0	36.0	36.0
21	34.97924352729286	36.0	36.0	36.0	36.0	36.0
22	34.965916616385336	36.0	36.0	36.0	36.0	36.0
23	34.909424551401855	36.0	36.0	36.0	32.0	36.0
24	34.87885614407667	36.0	36.0	36.0	32.0	36.0
25	34.851891577980965	36.0	36.0	36.0	32.0	36.0
26	34.78866049404378	36.0	36.0	36.0	32.0	36.0
27	34.78909293235058	36.0	36.0	36.0	32.0	36.0
28	34.76639558143142	36.0	36.0	36.0	32.0	36.0
29	34.73066791341725	36.0	36.0	36.0	32.0	36.0
30	34.716419524023536	36.0	36.0	36.0	32.0	36.0
31	34.70455577152315	36.0	36.0	36.0	32.0	36.0
32	34.67070049345512	36.0	36.0	36.0	32.0	36.0
33	34.64475589310397	36.0	36.0	36.0	32.0	36.0
34	34.643501029588066	36.0	36.0	36.0	32.0	36.0
35	34.59826820910543	36.0	36.0	36.0	32.0	36.0
36	34.5723632300651	36.0	36.0	36.0	32.0	36.0
37	34.56829581949889	36.0	36.0	36.0	32.0	36.0
38	34.52627911741832	36.0	36.0	36.0	32.0	36.0
39	34.528503571012145	36.0	36.0	36.0	32.0	36.0
40	34.50672883061663	36.0	36.0	36.0	32.0	36.0
41	34.495229594175896	36.0	36.0	36.0	32.0	36.0
42	34.42433461668646	36.0	36.0	36.0	32.0	36.0
43	34.4110869484006	36.0	36.0	36.0	32.0	36.0
44	34.34566197588081	36.0	36.0	36.0	32.0	36.0
45	34.32294764439987	36.0	36.0	36.0	32.0	36.0
46	34.30424242355634	36.0	36.0	36.0	32.0	36.0
47	34.279417408245536	36.0	36.0	36.0	32.0	36.0
48	34.2366377129221	36.0	36.0	36.0	32.0	36.0
49	34.220339769720944	36.0	36.0	36.0	32.0	36.0
50	33.608719178865314	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	15.0
21	60.0
22	190.0
23	600.0
24	1479.0
25	3398.0
26	6802.0
27	12211.0
28	20480.0
29	31342.0
30	45269.0
31	63783.0
32	91033.0
33	146061.0
34	303862.0
35	1040133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89063173970979	17.591880818439222	11.563516440221852	26.953971001629135
2	16.782191611790903	20.167508340323696	35.55660835515345	27.493691692731947
3	18.402746332186197	23.67851159210288	28.328993841695343	29.589748234015577
4	12.937999440773499	15.44908491752541	34.804321666178005	36.80859397552309
5	15.050664336178897	35.977848291132865	32.74774922653541	16.223738146152826
6	35.225705882752486	34.74458942087163	16.17689010847195	13.85281458790394
7	30.950469965348333	29.837620547838206	20.525650270613554	18.686259216199907
8	28.824730037368557	31.875910582625718	19.104037637981214	20.195321742024515
9	27.833937000163584	13.769207387999002	18.00200712853264	40.39484848330478
10	16.5644331516205	25.89178624412537	30.543095148205108	27.000685456049023
11	38.20453098246756	20.584131656030223	21.77373388614527	19.437603475356944
12	25.30749036916957	23.088748541083433	27.793337038877652	23.81042405086935
13	29.847405888788952	18.623907788078768	24.879479575913006	26.649206747219274
14	24.363893645021157	18.92009226110459	24.082751984604283	32.63326210926998
15	25.7473475191436	26.395920409725083	21.52211419592358	26.334617875207737
16	26.731068787668843	25.011490271193043	22.715077499898115	25.542363441239996
17	25.031329136493152	25.161513443510763	24.21597916145458	25.591178258541508
18	26.044674827165792	23.99477676736917	25.032744257444012	24.927804148021025
19	26.38711379127267	24.12094461733172	24.585645991511985	24.906295599883627
20	26.729913778123425	23.344319787900865	23.896868832147238	26.028897601828472
21	27.84489502050686	23.41370421899038	23.536756690058333	25.204644070444427
22	27.013372199974526	23.255005730942845	24.069903352247803	25.66171871683482
23	25.369795649449628	23.0975044701506	24.742984028586324	26.789715851813444
24	25.793235936218494	23.928706262852213	24.348976832195728	25.92908096873357
25	25.8077352169215	23.497947301719194	24.41293055579926	26.281386925560042
26	25.684730095746456	24.45375273470335	24.550999210367674	25.310517959182516
27	26.37834074384352	23.709141956318085	23.966580533047292	25.945936766791107
28	25.603794731300873	23.659900603398505	24.81490496190552	25.92139970339511
29	25.545185269879937	23.897417378118327	24.71595234712539	25.84144500487634
30	25.283796794985363	24.271043415603035	24.92266519647993	25.52249459293167
31	26.20426106163131	23.812437662812563	23.77898360807924	26.204317667476886
32	25.85100204790675	23.818095163702353	23.687681201257497	26.643221587133393
33	25.463656622683555	23.459709067320706	24.418409170283184	26.658225139712556
34	26.407236130843554	23.468350418013348	24.43620555954921	25.688207891593883
35	26.734522681501254	23.41134397701454	24.51901360856113	25.335119732923072
36	25.510659820320196	24.177184593795833	24.171014504238965	26.141141081645003
37	26.56127860384213	23.871992446836632	24.066878099392248	25.49985084992899
38	25.61960688416872	23.70650128721547	24.43694229153789	26.23694953707792
39	26.348149024460238	23.687086897727525	23.806464503982934	26.158299573829304
40	26.410325785482858	23.860464010541783	24.430062195616195	25.29914800835916
41	25.3982207213454	24.284940433164977	24.71416579544239	25.602673050047237
42	26.729933361672405	24.213038878678393	24.161813459096926	24.895214300552272
43	26.05588161877037	23.22998994169352	24.542154605849667	26.17197383368644
44	25.672409557673998	23.980900047885726	24.049219281532977	26.2974711129073
45	25.70074149544348	24.208354559347935	24.234674817456277	25.856229127752307
46	25.521124305134574	23.90246497866938	24.445508569888986	26.13090214630706
47	25.70562547581784	23.76980746535197	24.738961733632575	25.785605325197615
48	26.521620510986004	24.38764432258525	23.776901820454157	25.31383334597459
49	25.59693851271535	24.480521281491214	23.878446140113645	26.04409406567979
50	25.704459534648887	24.59527856596644	23.94287910459504	25.757382794789635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	27.5
2	34.0
3	106.0
4	178.0
5	224.5
6	271.0
7	244.5
8	218.0
9	312.5
10	407.0
11	582.0
12	757.0
13	1417.5
14	2078.0
15	2704.0
16	3330.0
17	3371.5
18	3413.0
19	3325.5
20	3238.0
21	3562.0
22	3886.0
23	4365.5
24	4845.0
25	5700.0
26	6555.0
27	8110.0
28	9665.0
29	11544.5
30	13424.0
31	15353.5
32	17283.0
33	20781.0
34	24279.0
35	29169.0
36	34059.0
37	43054.0
38	52049.0
39	58513.0
40	64977.0
41	73384.5
42	81792.0
43	86000.5
44	90209.0
45	100713.5
46	111218.0
47	120605.0
48	129992.0
49	136614.5
50	143237.0
51	139569.0
52	135901.0
53	136394.5
54	136888.0
55	138408.0
56	139928.0
57	136991.5
58	134055.0
59	123567.0
60	113079.0
61	99569.0
62	86059.0
63	75680.5
64	65302.0
65	56230.5
66	47159.0
67	40701.5
68	34244.0
69	30960.0
70	27676.0
71	21517.5
72	15359.0
73	13225.5
74	11092.0
75	8768.0
76	6444.0
77	5575.5
78	4707.0
79	3960.5
80	3214.0
81	2560.0
82	1906.0
83	1557.0
84	1208.0
85	872.0
86	536.0
87	382.0
88	228.0
89	181.0
90	134.0
91	111.0
92	88.0
93	61.5
94	35.0
95	36.5
96	38.0
97	28.0
98	18.0
99	15.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008150669656754924
2	4.528149809308291E-4
3	3.3961123569812185E-4
4	0.0
5	0.0
6	1.1320374523270727E-4
7	0.0
8	0.0
9	0.0013018430701761338
10	0.0010754355797107191
11	5.6601872616353635E-5
12	2.2640749046541454E-4
13	7.358243440125973E-4
14	5.6601872616353635E-5
15	0.004414946064075584
16	7.924262166289509E-4
17	0.0
18	2.2640749046541454E-4
19	0.0
20	2.830093630817682E-4
21	0.0
22	5.6601872616353635E-5
23	3.9621310831447547E-4
24	3.9621310831447547E-4
25	0.0015282505606415483
26	0.004584751681924645
27	0.005150770408088181
28	0.0037357235926793403
29	0.0018678617963396702
30	0.0032263067391321574
31	0.007018632204427852
32	0.0024904823951195604
33	0.0035659179748302795
34	0.002037667414188731
35	0.0024904823951195604
36	0.007867660293673157
37	0.0027734917582013284
38	0.006905428459195144
39	0.0035659179748302795
40	0.002377278649886853
41	0.0031131029938994506
42	0.001415046815408841
43	0.0015282505606415483
44	0.0011320374523270727
45	0.0014716486880251946
46	9.622318344780118E-4
47	0.001754658051106963
48	0.0013584449427924874
49	1.6980561784906093E-4
50	9.622318344780118E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1766726.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.92256178001307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.82393784401548	41.18642158513806
2	12.853406549947321	13.604704044464237
3	3.8951773458619594	6.1842829119146066
4	1.7368851890614274	3.6768165489157227
5	0.9432634979910111	2.4959960373630254
6	0.5983575885276349	1.899996986723604
7	0.39902203237894684	1.4782087712112832
8	0.2875050696790546	1.2172403849725384
9	0.2231681213401286	1.0629565820057167
>10	1.0896119062747809	9.984101904440122
>50	0.06705082226811493	2.4666052872276736
>100	0.06775050603821657	7.831849216830024
>500	0.010662964746250291	3.822953301232272
>1k	0.004200561869734963	3.0878664375612015
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2107	0.11926014560265713	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2044	0.11569422762782684	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1914	0.10833598418770088	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1904	0.10776996546153733	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1866	0.10561909430211588	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1855	0.104996473703336	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1803	0.1020531763272856	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1782	0.10086453700234219	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1320374523270728E-4
2	0.0	0.0	0.0	0.0	1.1320374523270728E-4
3	0.0	0.0	0.0	0.0	1.1320374523270728E-4
4	0.0	0.0	0.0	0.0	1.1320374523270728E-4
5	0.0	0.0	0.0	0.0	1.1320374523270728E-4
6	0.0	0.0	0.0	0.0	1.6980561784906093E-4
7	0.0	0.0	0.0	0.0	1.6980561784906093E-4
8	0.0	0.0	0.0	0.0	1.6980561784906093E-4
9	0.0	0.0	0.0	0.0	1.6980561784906093E-4
10	0.0	0.0	0.0	0.0	1.6980561784906093E-4
11	0.0	0.0	0.0	0.0	1.6980561784906093E-4
12	0.0	0.0	0.0	0.0	1.6980561784906093E-4
13	0.0	0.0	0.0	0.0	1.6980561784906093E-4
14	0.0	0.0	0.0	0.0	1.6980561784906093E-4
15	0.0	0.0	0.0	0.0	2.2640749046541457E-4
16	0.0	0.0	0.0	0.0	2.830093630817682E-4
17	0.0	0.0	0.0	0.0	2.830093630817682E-4
18	0.0	0.0	0.0	0.0	3.3961123569812185E-4
19	0.0	0.0	0.0	0.0	3.3961123569812185E-4
20	0.0	0.0	0.0	1.1320374523270728E-4	3.3961123569812185E-4
21	0.0	0.0	0.0	1.1320374523270728E-4	3.3961123569812185E-4
22	0.0	0.0	0.0	1.1320374523270728E-4	4.5281498093082914E-4
23	0.0	0.0	0.0	1.6980561784906093E-4	5.660187261635364E-4
24	0.0	0.0	0.0	5.094168535471828E-4	5.660187261635364E-4
25	0.0	0.0	0.0	5.660187261635364E-4	5.660187261635364E-4
26	0.0	0.0	0.0	7.924262166289509E-4	6.2262059877989E-4
27	0.0	0.0	0.0	0.0011886393249434263	6.2262059877989E-4
28	0.0	0.0	0.0	0.003396112356981218	6.2262059877989E-4
29	0.0	0.0	0.0	0.008433679019836693	6.2262059877989E-4
30	0.0	0.0	0.0	0.018452210472931288	6.2262059877989E-4
31	0.0	0.0	0.0	0.042055191353950756	6.2262059877989E-4
32	0.0	0.0	0.0	0.06769583964915896	6.2262059877989E-4
33	0.0	0.0	0.0	0.09775143400844273	6.2262059877989E-4
34	0.0	0.0	0.0	0.12910887143790264	6.2262059877989E-4
35	0.0	0.0	0.0	0.16754154294440676	6.2262059877989E-4
36	0.0	0.0	0.0	0.22040769196808108	6.2262059877989E-4
37	0.0	0.0	0.0	0.2926882832991647	6.2262059877989E-4
38	0.0	0.0	0.0	0.3941754409002867	6.792224713962437E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2385	0.0	25.733995	1
GTATTAC	135	1.1459633E-10	21.18366	1
GTTCGCG	75	5.470347E-5	20.535341	38
GGTATCA	1045	0.0	20.41958	1
TATCGTT	65	4.937684E-4	20.30508	17
TACCGTA	65	4.937684E-4	20.30508	7
TATACTG	275	0.0	18.397633	5
ACCGTCG	490	0.0	17.956873	8
GTACCGT	160	1.1041266E-9	17.8727	6
AACGCAG	3425	0.0	17.790411	6
ATACCGT	550	0.0	17.197788	6
TCAACGC	3585	0.0	17.180496	4
TTAACGG	155	1.438093E-8	17.034407	35
ATCAACG	3625	0.0	16.990917	3
GTAAACG	520	0.0	16.92569	27
CAACGCA	3675	0.0	16.75975	5
TATCAAC	3720	0.0	16.734406	2
TACCGTC	540	0.0	16.701555	7
CGTCGTA	545	0.0	16.549267	10
TAAACGC	535	0.0	16.450674	28
>>END_MODULE