FastQCFastQC Report
Thu 2 Feb 2017
SRR4064022_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064022_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673907
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA116390.6953193934907973No Hit
GTCCTAAAGTGTGTATTTCTCATTT115850.6920934078177581No Hit
GTCCTACAGTGGACATTTCTAAATT107710.6434646608204637No Hit
CTTTAGGACGTGAAATATGGCGAGG92380.5518825119914069No Hit
GTCCTACAGTGTGCATTTCTCATTT64540.38556502840360907No Hit
CTGAAGGACCTGGAATATGGCGAGA49000.29272832959059253No Hit
ATTTAGAAATGTCCACTGTAGGACG47220.2820945249646486No Hit
CTGTAGGACCTGGAATATGGCGAGA46730.2791672416687427No Hit
GTCCTTCAGTGTGCATTTCTCATTT43580.2603489919093474No Hit
GATATACACTGTTCTACAAATCCCG40610.24260607070763193No Hit
TTGTAGAACAGTGTATATCAATGAG33600.2007279974335492No Hit
GAATATGGCAAGAAAACTGAAAATC32300.19296173562808447No Hit
TTTCTAAATTTTCCACCTTTTTCAG30230.1805954572147676No Hit
GGAATATGGCGAGAAAACTGAAAAT26440.15795381702806666No Hit
GTGTATATCAATGAGTTACAATGAG25840.1543693885024676No Hit
ATTCCAGGTCCTTCAGTGTGCATTT25400.1517408075836949No Hit
CCATATTCCAGGTCCTTCAGTGTGC23470.14021089582635118No Hit
GTATCAACGCAGAGTACTTTTTTTT23390.13973297202293794No Hit
GCCATATTCCACGTCCTACAGTGGA23100.13800049823556507No Hit
TTCCAGGTCCTTCAGTGTGCATTTC22920.13692516967788532No Hit
ACCTGGAATATGGCGAGAAAACTGA22420.13393814590655276No Hit
GTGTATATCAATGAGTTACAATGAA22240.132862817348873No Hit
CTGTAGGACATGGAATATGGCAAGA21740.12987579357754045No Hit
GACCTGGAATATGGCGAGAAAACTG21740.12987579357754045No Hit
GAAATATGGCGAGGAAAACTGAAAA21650.1293381292987006No Hit
GTTCTACAGTGTGGTTTTTATCATT21270.12706799123248783No Hit
CCATATTTCACGTCCTAAAGTGTGT20540.12270693652634226No Hit
GTCCACTGTAGGACGTGGAATATGG19530.11667314850825046No Hit
CCACTGTAGGACGTGGAATATGGCA19050.11380560568777119No Hit
GTTCTACAAATCCCGTTTCCAACGA19000.11350690331063792No Hit
ATACACACTTTAGGACGTGAAATAT18470.11034065811302539No Hit
ACAGTGGACATTTCTAAATTTTCCA17350.10364972486524042No Hit
CCTAAAGTGTGTATTTCTCATTTTC17110.10221595345500079No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT350.002169540616.2865587
GGTATCA6750.015.6188321
ACGCGCG508.7107124E-515.20078913
CGCATCG1700.014.53016613
GCGTTAT2300.014.4533841
CGCCAGT1650.014.39683918
CGAACGA1152.5465852E-1114.04546816
TAGAAAT13550.014.0203534
GGTTCGT551.956947E-413.82096518
ACGATCA707.259445E-613.5721329
GTTCAAA2800.013.5684821
ATCGCCA1850.013.35324216
AAATGTC13750.013.2661437
CGTTATT2450.013.179632
TAGGACC18000.013.034454
TCGCCAG1900.013.00184117
CTAGGAC3000.012.9797393
TTAGAAA13850.012.960313
TCCAACG4700.012.93877618
CACCTTT9700.012.92927914
Produced by FastQC (version 0.11.5)