FastQCFastQC Report
Thu 2 Feb 2017
SRR4064022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673907
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA59000.3524688050172441No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA41630.24869959920115034No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC40640.24278529213391187No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA40530.2421281469042187No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA25880.1546083504041742No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG24950.1490524861894956No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA24390.1457070195656031No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA17480.10442635104578687No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC17280.10323154153725386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT502.3624452E-630.7995414
GGTATCA7050.030.5811021
GTATCAA21200.028.4335371
ACCGATG551.5941366E-423.9996438
TAGGACC24400.023.6225974
TGTAGGA67550.022.1781312
CCGTAGG500.002580624522.000339
ATATGCG703.2166783E-521.9996715
CTGTAGG66000.021.9330061
GTCCTAC59300.021.4060881
ACTGTTC17850.021.3218128
GATATAC18350.021.2203941
GTAGGAC70450.021.109063
ATACACT18450.021.1053754
GTCCTAA33600.020.6246931
TCCTACA61500.020.6039542
CCGTCCA755.47362E-520.5336389
CCTACAG60200.020.4276563
TAGGACA15850.020.403484
TTAGGAC37450.020.3248563
Produced by FastQC (version 0.11.5)