##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1673907
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39240650764947	32.0	32.0	32.0	32.0	32.0
2	31.47060559517345	32.0	32.0	32.0	32.0	32.0
3	31.517963064853664	32.0	32.0	32.0	32.0	32.0
4	31.58718793815905	32.0	32.0	32.0	32.0	32.0
5	31.567391139412166	32.0	32.0	32.0	32.0	32.0
6	35.15810436302614	36.0	36.0	36.0	36.0	36.0
7	35.168109697850596	36.0	36.0	36.0	36.0	36.0
8	35.11491259669743	36.0	36.0	36.0	36.0	36.0
9	35.20999255036271	36.0	36.0	36.0	36.0	36.0
10	35.09028339089328	36.0	36.0	36.0	36.0	36.0
11	35.201662935873976	36.0	36.0	36.0	36.0	36.0
12	35.11898868933579	36.0	36.0	36.0	36.0	36.0
13	35.15229997843369	36.0	36.0	36.0	36.0	36.0
14	35.10920558907992	36.0	36.0	36.0	36.0	36.0
15	35.08322445631687	36.0	36.0	36.0	36.0	36.0
16	35.10261740944987	36.0	36.0	36.0	36.0	36.0
17	35.09231277484353	36.0	36.0	36.0	36.0	36.0
18	35.08797740854181	36.0	36.0	36.0	36.0	36.0
19	35.048322278358356	36.0	36.0	36.0	36.0	36.0
20	35.04375571641674	36.0	36.0	36.0	36.0	36.0
21	35.02116903746743	36.0	36.0	36.0	36.0	36.0
22	34.987237642234604	36.0	36.0	36.0	36.0	36.0
23	34.9727720835148	36.0	36.0	36.0	36.0	36.0
24	34.945087152392574	36.0	36.0	36.0	36.0	36.0
25	34.89496011427158	36.0	36.0	36.0	36.0	36.0
26	34.85549436139523	36.0	36.0	36.0	32.0	36.0
27	34.81354818397916	36.0	36.0	36.0	32.0	36.0
28	34.75879305122686	36.0	36.0	36.0	32.0	36.0
29	34.73201677273588	36.0	36.0	36.0	32.0	36.0
30	34.67577529695497	36.0	36.0	36.0	32.0	36.0
31	34.68247877570259	36.0	36.0	36.0	32.0	36.0
32	34.64004989524508	36.0	36.0	36.0	32.0	36.0
33	34.676569845278145	36.0	36.0	36.0	32.0	36.0
34	34.65303030574578	36.0	36.0	36.0	32.0	36.0
35	34.63223046441648	36.0	36.0	36.0	32.0	36.0
36	34.57754940985372	36.0	36.0	36.0	32.0	36.0
37	34.50522520068319	36.0	36.0	36.0	32.0	36.0
38	34.44215598596577	36.0	36.0	36.0	32.0	36.0
39	34.32294267244237	36.0	36.0	36.0	32.0	36.0
40	34.390417149817765	36.0	36.0	36.0	32.0	36.0
41	34.380321009470656	36.0	36.0	36.0	32.0	36.0
42	34.347244500441185	36.0	36.0	36.0	32.0	36.0
43	34.37219630481263	36.0	36.0	36.0	32.0	36.0
44	34.23920922727487	36.0	36.0	36.0	32.0	36.0
45	34.22745648354419	36.0	36.0	36.0	32.0	36.0
46	34.092774568718575	36.0	36.0	36.0	32.0	36.0
47	34.13337001398524	36.0	36.0	36.0	32.0	36.0
48	34.06238518627379	36.0	36.0	36.0	32.0	36.0
49	34.04090310871512	36.0	36.0	36.0	32.0	36.0
50	33.54201398285568	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	10.0
21	49.0
22	116.0
23	442.0
24	1179.0
25	2842.0
26	5768.0
27	11356.0
28	19278.0
29	30090.0
30	43686.0
31	62440.0
32	89499.0
33	142511.0
34	286978.0
35	977659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.92816315654576	20.031676822328397	12.327194928362704	26.71296509276314
2	12.767922537693455	20.714404342439025	44.035000937930505	22.482672181937012
3	18.89056627669889	25.248655982230705	29.761592545308886	26.099185195761514
4	11.085382879694034	17.062357705655092	38.96303677563927	32.88922263901161
5	11.559722254581645	39.72968629678949	35.76190314037757	12.948688308251294
6	31.996358211915503	38.4220591037832	17.525746712921837	12.055835971379466
7	27.902718493582373	31.122196301462747	22.121386817053537	18.853698387901343
8	23.85974848065036	41.20228901605645	19.422524668335818	15.515437834957376
9	26.756736573898444	14.151211104705522	19.99820776218199	39.09384455921404
10	15.712152956378828	28.44907876209235	33.910133826078074	21.928634455450748
11	35.736931621649234	20.001768318072628	27.07545879191616	17.185841268361983
12	26.0884448432465	23.058040434864168	32.39341371239177	18.46010100949757
13	30.798177431042816	21.709348402324867	25.73760335886053	21.754870807771788
14	19.97225644034119	24.1934713023821	25.989932505009257	29.84433975226746
15	21.409444810874366	36.72535918098133	21.44684456042748	20.418351447716823
16	20.675047508896903	26.809893320795723	31.67823348748459	20.83682568282278
17	20.23565227936797	30.084467058205743	28.816535207750487	20.863345454675798
18	20.816464056118015	26.096346199685645	34.17752901754644	18.909660726649903
19	25.468738705316362	24.58422122614936	26.074148683290048	23.872891385244223
20	25.117734526639396	27.882726076278473	27.363995189656688	19.635544207425447
21	23.46002495957063	25.681116095458112	25.36246039953235	25.496398545438904
22	24.541266728119847	30.177316128831837	26.01321581955466	19.268201323493656
23	20.842072940146114	29.662170760018714	28.49196454014683	21.003791759688344
24	24.999029213794604	26.21959124225387	28.874527227117973	19.90685231683355
25	22.551323747643206	28.36421161800937	28.74332988426916	20.34113475007826
26	18.763655264502137	30.580384214409683	30.426722348182732	20.229238172905443
27	21.026111321876336	28.99584477191054	28.622979516607472	21.35506438960566
28	19.82320795827058	29.75539346564856	27.123407321288706	23.29799125479215
29	22.03787774239049	26.613890417293213	26.48389141498776	24.86434042532854
30	19.81781977576072	27.939219888196998	28.76683930394656	23.47612103209572
31	24.075312122673157	25.780851855768468	27.578100121819997	22.565735899738375
32	21.314941446506086	29.396171948091055	27.4791588752663	21.809727730136558
33	19.931749219005308	29.38212849954028	30.415389404042354	20.270732877412062
34	22.02736311720402	28.821065573623585	29.72908709753928	19.42248421163312
35	20.800544848434157	29.737851433214246	29.372767137036547	20.088836581315046
36	19.174003934850916	28.885067820316042	30.291481280780232	21.649446964052814
37	20.857394801647473	28.458130356701183	26.501190676756053	24.183284164895287
38	21.50913839056067	30.382377911329144	26.684184081019836	21.42429961709035
39	24.34572521355113	26.061183937752634	27.388313564395123	22.20477728430111
40	25.475948237287387	26.46258458696589	28.540047040718242	19.52142013502849
41	23.817793924273573	25.32265609457529	30.567061029094162	20.292488952056974
42	22.64713982652146	29.82268005761509	28.73537742245949	18.794802693403955
43	19.89010017396624	29.618947026324342	29.940414173469193	20.550538626240225
44	19.27864731286476	29.61270291890174	28.423193257832235	22.685456510401263
45	19.84624970801424	30.0817918576149	28.257291578921585	21.814666855449275
46	21.013177145595	26.24645808578019	28.533177050008874	24.207187718615934
47	24.012500343514507	26.48299692808419	28.146443519643654	21.35805920875765
48	20.722370879102826	28.079721508808593	27.0539591618739	24.14394845021468
49	22.938173276364715	26.963135281354244	27.622073906269417	22.476617536011624
50	20.723981738258733	30.922550096123864	26.139596459009656	22.213871706607755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	13.5
2	15.0
3	51.0
4	87.0
5	113.0
6	139.0
7	151.0
8	163.0
9	219.0
10	275.0
11	439.5
12	604.0
13	1053.5
14	1503.0
15	1976.5
16	2450.0
17	2724.5
18	2999.0
19	3280.5
20	3562.0
21	4488.5
22	5415.0
23	6475.5
24	7536.0
25	11379.5
26	15223.0
27	23753.5
28	32284.0
29	42889.5
30	53495.0
31	66208.5
32	78922.0
33	100435.5
34	121949.0
35	146152.5
36	170356.0
37	168252.0
38	166148.0
39	148489.5
40	130831.0
41	116433.5
42	102036.0
43	90637.5
44	79239.0
45	79124.5
46	79010.0
47	80592.0
48	82174.0
49	82509.0
50	82844.0
51	77450.0
52	72056.0
53	69641.0
54	67226.0
55	66836.5
56	66447.0
57	64000.5
58	61554.0
59	56348.0
60	51142.0
61	44845.5
62	38549.0
63	33740.5
64	28932.0
65	24982.0
66	21032.0
67	18272.5
68	15513.0
69	14229.0
70	12945.0
71	9651.5
72	6358.0
73	5531.0
74	4704.0
75	3707.5
76	2711.0
77	2360.0
78	2009.0
79	1673.0
80	1337.0
81	1131.0
82	925.0
83	756.0
84	587.0
85	445.0
86	303.0
87	220.0
88	137.0
89	99.5
90	62.0
91	50.0
92	38.0
93	31.0
94	24.0
95	23.5
96	23.0
97	15.5
98	8.0
99	11.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007646780854611397
2	5.376642788398638E-4
3	4.779238034132123E-4
4	0.0
5	0.0
6	2.3896190170660616E-4
7	1.1948095085330308E-4
8	0.0
9	0.001314290459386334
10	0.0010753285576797277
11	0.0
12	2.987023771332577E-4
13	3.584428525599092E-4
14	2.987023771332577E-4
15	0.005914307067238503
16	5.974047542665154E-4
17	0.0
18	3.584428525599092E-4
19	0.0
20	8.363666559731216E-4
21	0.0
22	2.3896190170660616E-4
23	8.363666559731216E-4
24	3.584428525599092E-4
25	0.0013740309348129855
26	0.006093528493518457
27	0.006690933247784973
28	0.004540276132425517
29	0.0028675428204792738
30	0.0041818332798656075
31	0.007527299903758094
32	0.003166245197612532
33	0.004779238034132123
34	0.0028675428204792738
35	0.002807802345052622
36	0.008722109412291124
37	0.004122092804438956
38	0.008602628461437821
39	0.004122092804438956
40	0.0022701380662127585
41	0.003942871378159001
42	0.0023896190170660616
43	0.0011350690331063792
44	0.0014337714102396369
45	0.0014337714102396369
46	0.0014337714102396369
47	0.0019714356890795006
48	0.0017922142627995463
49	1.792214262799546E-4
50	0.0014935118856662885
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1673907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.782893694077444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54472315061422	31.59196837532081
2	15.101446364430787	13.525729343302325
3	5.964708386687281	8.013507047915661
4	2.893172884078107	5.182586148250293
5	1.5888861718363168	3.5577460262667717
6	0.9509275936001392	2.5551173600975954
7	0.6257245321262764	1.9615228642791174
8	0.4222726618643438	1.5128473380948817
9	0.288510761931632	1.1628312103063407
>10	1.3878902173997443	11.16072215267095
>50	0.11690061997277239	3.6616815623685803
>100	0.10048227765488801	9.329414819930449
>500	0.009256561387385958	2.936293865803386
>1k	0.0049636633526562385	3.4935739046615386
>5k	1.3415306358530374E-4	0.35445798073125273
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	5900	0.3524688050172441	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	4163	0.24869959920115034	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	4064	0.24278529213391187	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	4053	0.2421281469042187	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2588	0.1546083504041742	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	2495	0.1490524861894956	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	2439	0.1457070195656031	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	1748	0.10442635104578687	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	1728	0.10323154153725386	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.974047542665154E-5	0.0
4	0.0	0.0	0.0	5.974047542665154E-5	0.0
5	0.0	0.0	0.0	1.1948095085330308E-4	0.0
6	0.0	0.0	0.0	1.1948095085330308E-4	0.0
7	0.0	0.0	0.0	1.1948095085330308E-4	0.0
8	0.0	0.0	0.0	1.1948095085330308E-4	0.0
9	0.0	0.0	0.0	1.1948095085330308E-4	0.0
10	0.0	0.0	0.0	1.1948095085330308E-4	0.0
11	0.0	0.0	0.0	1.1948095085330308E-4	0.0
12	0.0	0.0	0.0	1.1948095085330308E-4	0.0
13	0.0	0.0	0.0	1.1948095085330308E-4	0.0
14	0.0	0.0	0.0	1.1948095085330308E-4	0.0
15	0.0	0.0	0.0	1.1948095085330308E-4	0.0
16	0.0	0.0	0.0	1.792214262799546E-4	0.0
17	0.0	0.0	0.0	1.792214262799546E-4	0.0
18	0.0	0.0	0.0	1.792214262799546E-4	0.0
19	0.0	0.0	0.0	1.792214262799546E-4	0.0
20	0.0	0.0	0.0	2.3896190170660616E-4	0.0
21	0.0	0.0	0.0	2.987023771332577E-4	0.0
22	0.0	0.0	0.0	4.779238034132123E-4	0.0
23	0.0	0.0	0.0	5.974047542665154E-4	0.0
24	0.0	0.0	0.0	6.57145229693167E-4	0.0
25	0.0	0.0	0.0	6.57145229693167E-4	0.0
26	0.0	0.0	0.0	6.57145229693167E-4	0.0
27	0.0	0.0	0.0	9.558476068264246E-4	0.0
28	0.0	0.0	0.0	0.003405207099319138	0.0
29	0.0	0.0	0.0	0.009677957019117549	0.0
30	0.0	0.0	0.0	0.022342937809567677	0.0
31	0.0	0.0	0.0	0.054423573113679555	0.0
32	0.0	0.0	0.0	0.09164188930448346	0.0
33	0.0	0.0	0.0	0.12730695313419443	0.0
34	0.0	0.0	0.0	0.1661980026369446	0.0
35	0.0	0.0	0.0	0.2049098307134148	0.0
36	0.0	0.0	0.0	0.2693698036987718	0.0
37	0.0	0.0	0.0	0.37630525471247805	0.0
38	0.0	0.0	0.0	0.5154408219811495	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGT	50	2.3624452E-6	30.799541	4
GGTATCA	705	0.0	30.581102	1
GTATCAA	2120	0.0	28.433537	1
ACCGATG	55	1.5941366E-4	23.999643	8
TAGGACC	2440	0.0	23.622597	4
TGTAGGA	6755	0.0	22.178131	2
CCGTAGG	50	0.0025806245	22.00033	9
ATATGCG	70	3.2166783E-5	21.99967	15
CTGTAGG	6600	0.0	21.933006	1
GTCCTAC	5930	0.0	21.406088	1
ACTGTTC	1785	0.0	21.321812	8
GATATAC	1835	0.0	21.220394	1
GTAGGAC	7045	0.0	21.10906	3
ATACACT	1845	0.0	21.105375	4
GTCCTAA	3360	0.0	20.624693	1
TCCTACA	6150	0.0	20.603954	2
CCGTCCA	75	5.47362E-5	20.533638	9
CCTACAG	6020	0.0	20.427656	3
TAGGACA	1585	0.0	20.40348	4
TTAGGAC	3745	0.0	20.324856	3
>>END_MODULE