FastQCFastQC Report
Thu 2 Feb 2017
SRR4064021_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064021_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166734
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36270.31086777277425703No Hit
TATCAACGCAGAGTACTTTTTTTTT26720.22901535397099937No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22310.19121753544509718No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15150.1298496486774192No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14610.12522134436812504No Hit
GCGCAAGACGGACCAGAGCGAAAGC14140.12119300543225792No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13620.11673611980108577No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13500.11570760773235372No Hit
GAATAGGACCGCGGTTCTATTTTGT13110.11236494350897464No Hit
GTACATGGGGTGGTATCAACGCAAA13000.1114221407793036No Hit
GTATCTGATCGTCTTCGAACCTCCG12900.11056504738869356No Hit
GATTAAGAGGGACGGCCGGGGGCAT12890.11047933804963256No Hit
GAACTACGACGGTATCTGATCGTCT12710.10893656994653451No Hit
GAATAACGCCGCCGCATCGCCAGTC12550.10756522052155847No Hit
GGGTAGGCACACGCTGAGCCAGTCA12490.10705096448719247No Hit
GTACATGGGGAATAATTGCAATCCC12200.10456539365442338No Hit
GTCCTATTCCATTATTCCTAGCTGC12020.10302262555132533No Hit
CTATTGGAGCTGGAATTACCGCGGC11820.10130843877010527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC250.00602812919.001678
GCGTTAT1450.016.3744321
CGTTATT1550.015.3160462
GACCGTC456.760421E-414.7778117
GTTATTC1850.013.8607363
CGCCAGT2650.013.264750518
ATCGCCA2750.013.12899216
AAGACGG2750.013.1261775
CGTCTTA801.9942836E-613.06477115
TAGAACC1403.6379788E-1212.8912294
TCGCCAG2950.012.88193717
CGCATCG2700.012.66832513
TAACGAA751.47584415E-512.66832513
GCATCGC2700.012.66832514
CCGATAA751.4764953E-512.6677839
CGATAAC751.4764953E-512.66778310
AGAACCG1352.7284841E-1112.6656095
TATTCCC2150.012.3710595
CGGTTCT2350.012.12924812
AGAGCGA2900.012.12279615
Produced by FastQC (version 0.11.5)