##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064021_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1166734
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.216628640289905	32.0	32.0	32.0	32.0	32.0
2	30.83123059754837	32.0	32.0	32.0	32.0	32.0
3	30.844938949237786	32.0	32.0	32.0	32.0	32.0
4	30.87412297918806	32.0	32.0	32.0	32.0	32.0
5	30.77129148546284	32.0	32.0	32.0	32.0	32.0
6	34.42721391508262	36.0	36.0	36.0	32.0	36.0
7	34.343802443401835	36.0	36.0	36.0	32.0	36.0
8	34.30799393863554	36.0	36.0	36.0	32.0	36.0
9	34.44377381648259	36.0	36.0	36.0	32.0	36.0
10	34.1514929709771	36.0	36.0	36.0	32.0	36.0
11	34.4273613351458	36.0	36.0	36.0	32.0	36.0
12	34.2400598594024	36.0	36.0	36.0	32.0	36.0
13	34.3333913299861	36.0	36.0	36.0	32.0	36.0
14	34.222942847298526	36.0	36.0	36.0	32.0	36.0
15	34.15983334676113	36.0	36.0	36.0	32.0	36.0
16	34.15622841196022	36.0	36.0	36.0	32.0	36.0
17	34.07078477185031	36.0	36.0	36.0	32.0	36.0
18	34.07743495946805	36.0	36.0	36.0	32.0	36.0
19	34.08453683530265	36.0	36.0	36.0	32.0	36.0
20	34.05672844024431	36.0	36.0	36.0	32.0	36.0
21	34.02815208950798	36.0	36.0	36.0	32.0	36.0
22	34.01661732665715	36.0	36.0	36.0	32.0	36.0
23	33.97192847727074	36.0	36.0	36.0	32.0	36.0
24	33.94766587756935	36.0	36.0	36.0	32.0	36.0
25	33.5613567445536	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	13.0
5	19.0
6	65.0
7	13.0
8	66.0
9	94.0
10	115.0
11	29.0
12	84.0
13	62.0
14	122.0
15	196.0
16	327.0
17	463.0
18	613.0
19	770.0
20	1201.0
21	1730.0
22	2781.0
23	4284.0
24	6485.0
25	9302.0
26	13272.0
27	17504.0
28	23815.0
29	32061.0
30	42892.0
31	58613.0
32	83067.0
33	116363.0
34	246565.0
35	503742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.1540967891572	16.864148604050357	10.991222421623865	25.990532185168576
2	16.88999402574672	19.50340841006863	37.1235127580736	26.48308480611105
3	19.26715356245034	23.58244672416734	27.83114241658817	29.31925729679415
4	12.63305862336449	14.724454330003386	35.24730325666311	37.39518378996902
5	14.83650477580749	36.456001968097276	33.177611350503895	15.529881905591333
6	35.0383221737129	34.805243121424844	16.517238334689175	13.639196370173082
7	30.87425767784226	29.78093191168692	20.53845843896091	18.80635197150992
8	28.73890914329027	32.40656585576656	19.168533005909406	19.685991995033767
9	27.65467734480384	13.77164119332385	18.352040654443236	40.221640807429075
10	16.184059424027417	26.196640974688528	31.113308570463115	26.50599103082094
11	37.92469124473759	20.59100229577901	22.25833645640464	19.225970003078764
12	25.373072882046444	23.141968823037505	28.542745030769563	22.942213264146496
13	29.802632030354804	19.093112533706595	25.22796978242523	25.876285653513374
14	23.824782934253715	19.498220674145355	24.844432283978517	31.832564107622414
15	25.268915880059488	27.368526678891026	22.083692831254105	25.27886460979538
16	25.920795544020297	25.16429452420203	23.98468471404776	24.930225217729916
17	24.02559308369213	25.684940906050052	25.170420433298457	25.119045576959365
18	25.05277605026699	24.353756085007827	26.511290772233064	24.082177092492117
19	25.902337095537337	24.273421135248917	25.229583652854764	24.594658116358982
20	26.06689833659164	23.878072192832185	24.864313538450716	25.190715932125457
21	27.253543960898487	23.600043232634462	24.220483004599473	24.92592980186758
22	26.20688886105788	24.062033032358123	24.813212943249333	24.917865163334664
23	24.52449009004063	23.878438289228065	25.656431865597888	25.940639755133414
24	25.06611606403758	24.575531866527474	25.259896393146747	25.0984556762882
25	25.029229031225952	24.26339839062338	25.362564366058127	25.34480821209254
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	129.0
2	341.5
3	554.0
4	554.0
5	554.0
6	1748.5
7	2943.0
8	2943.0
9	2943.0
10	2847.5
11	2752.0
12	2752.0
13	2752.0
14	2720.5
15	2689.0
16	2689.0
17	2689.0
18	4577.5
19	6466.0
20	6466.0
21	6466.0
22	10790.0
23	15114.0
24	15114.0
25	15114.0
26	23643.5
27	32173.0
28	32173.0
29	32173.0
30	40416.0
31	48659.0
32	48659.0
33	48659.0
34	59335.5
35	70012.0
36	70012.0
37	70012.0
38	79749.5
39	89487.0
40	89487.0
41	89487.0
42	103198.0
43	116909.0
44	116909.0
45	116909.0
46	131918.0
47	146927.0
48	146927.0
49	146927.0
50	151518.5
51	156110.0
52	156110.0
53	156110.0
54	145824.0
55	135538.0
56	135538.0
57	135538.0
58	126511.0
59	117484.0
60	117484.0
61	117484.0
62	105228.0
63	92972.0
64	92972.0
65	92972.0
66	77440.5
67	61909.0
68	61909.0
69	61909.0
70	46705.0
71	31501.0
72	31501.0
73	31501.0
74	24458.0
75	17415.0
76	17415.0
77	17415.0
78	14075.0
79	10735.0
80	10735.0
81	10735.0
82	7385.5
83	4036.0
84	4036.0
85	4036.0
86	3130.0
87	2224.0
88	2224.0
89	2224.0
90	1561.5
91	899.0
92	899.0
93	899.0
94	624.5
95	350.0
96	350.0
97	350.0
98	548.5
99	747.0
100	747.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040026261341488296
2	0.0052282696827211685
3	0.0019713147984030633
4	0.004199757613989136
5	0.010713667382625346
6	0.015170553013797489
7	0.02511283634487381
8	0.03925487728993927
9	0.0525398248443947
10	0.06351062024420305
11	0.05836805990054288
12	0.06933885530035125
13	0.06771037785819219
14	0.07225297282842533
15	0.0647962603301181
16	0.07199584481124231
17	0.06805321521443619
18	0.08219525615950166
19	0.07953826664861056
20	0.08528079236569776
21	0.08125245342983062
22	0.08296664021105067
23	0.08896629394532088
24	0.08408086161884372
25	0.0806524880564036
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1166734.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.81473778769904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.45824526590604	38.34390947106898
2	14.41581124955554	14.650713372862537
3	4.636643375255285	7.068294519860075
4	1.981509796957772	4.027596030246638
5	1.00941292463126	2.564652654232595
6	0.5906118939583728	1.8007073115474614
7	0.3699496448026444	1.315922593670914
8	0.24765302167496064	1.0067538686995579
9	0.18393167303933056	0.8411795762711737
>10	0.9118540089596403	8.404092667943198
>50	0.08281254148064282	2.9712960634723045
>100	0.09717463427488142	10.480656404797498
>500	0.010326919290844118	3.5487519632868114
>1k	0.004063050212791129	2.9754735020402463
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3627	0.31086777277425703	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2672	0.22901535397099937	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2231	0.19121753544509718	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1515	0.1298496486774192	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1461	0.12522134436812504	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1414	0.12119300543225792	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1362	0.11673611980108577	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1350	0.11570760773235372	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1311	0.11236494350897464	No Hit
GTACATGGGGTGGTATCAACGCAAA	1300	0.1114221407793036	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1290	0.11056504738869356	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1289	0.11047933804963256	No Hit
GAACTACGACGGTATCTGATCGTCT	1271	0.10893656994653451	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1255	0.10756522052155847	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1249	0.10705096448719247	No Hit
GTACATGGGGAATAATTGCAATCCC	1220	0.10456539365442338	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1202	0.10302262555132533	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1182	0.10130843877010527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7141867812200552E-4
2	0.0	0.0	0.0	0.0	1.7141867812200552E-4
3	0.0	0.0	0.0	0.0	1.7141867812200552E-4
4	0.0	0.0	0.0	0.0	1.7141867812200552E-4
5	0.0	0.0	0.0	0.0	1.7141867812200552E-4
6	0.0	0.0	0.0	0.0	4.2854669530501384E-4
7	0.0	0.0	0.0	0.0	4.2854669530501384E-4
8	0.0	0.0	0.0	0.0	4.2854669530501384E-4
9	0.0	0.0	0.0	0.0	4.2854669530501384E-4
10	0.0	0.0	0.0	0.0	5.142560343660166E-4
11	0.0	0.0	0.0	0.0	5.142560343660166E-4
12	0.0	0.0	0.0	0.0	6.856747124880221E-4
13	0.0	0.0	0.0	0.0	6.856747124880221E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTC	25	0.006028129	19.00167	8
GCGTTAT	145	0.0	16.374432	1
CGTTATT	155	0.0	15.316046	2
GACCGTC	45	6.760421E-4	14.777811	7
GTTATTC	185	0.0	13.860736	3
CGCCAGT	265	0.0	13.2647505	18
ATCGCCA	275	0.0	13.128992	16
AAGACGG	275	0.0	13.126177	5
CGTCTTA	80	1.9942836E-6	13.064771	15
TAGAACC	140	3.6379788E-12	12.891229	4
TCGCCAG	295	0.0	12.881937	17
CGCATCG	270	0.0	12.668325	13
TAACGAA	75	1.47584415E-5	12.668325	13
GCATCGC	270	0.0	12.668325	14
CCGATAA	75	1.4764953E-5	12.667783	9
CGATAAC	75	1.4764953E-5	12.667783	10
AGAACCG	135	2.7284841E-11	12.665609	5
TATTCCC	215	0.0	12.371059	5
CGGTTCT	235	0.0	12.129248	12
AGAGCGA	290	0.0	12.122796	15
>>END_MODULE