FastQCFastQC Report
Thu 2 Feb 2017
SRR4064021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166734
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14900.12770691520089414No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14210.12179297080568492No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14100.12085016807601391No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC13890.11905027195573284No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13240.11347916491676767No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12630.1082508952340465No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12500.10713667382625344No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA12290.1053367777059724No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11900.10199411348259328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG407.0329465E-427.4993575
TATACTG1500.023.4661185
GTATGCG703.2168733E-521.99854311
GATATAC2650.021.586251
TAGGACG4100.020.926344
TTAGGAC3150.020.2534943
ACCGTAT550.004484125419.9995338
GACGTGA2950.019.3893787
GTCGTGT708.1196864E-418.8583181
GGACGTG4700.018.7229676
GTATCAA29050.018.4039021
AGGACGT4550.018.3731985
GTCCTAC5150.018.3700771
TTATACT1701.2732926E-1018.1172244
TATGCGA750.001291406917.59883512
CCCTCGA3900.017.4882726
GATTTCG2055.456968E-1217.1703341
AACGCAG31050.017.1461376
TCCTACA5650.017.1323432
GTGTACG902.2142331E-417.1121791
Produced by FastQC (version 0.11.5)