##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064021_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1166734
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26230057579534	32.0	32.0	32.0	32.0	32.0
2	31.367477077037268	32.0	32.0	32.0	32.0	32.0
3	31.45554342292245	32.0	32.0	32.0	32.0	32.0
4	31.54231898616137	32.0	32.0	32.0	32.0	32.0
5	31.478104692243477	32.0	32.0	32.0	32.0	32.0
6	35.06652673188576	36.0	36.0	36.0	36.0	36.0
7	35.086333303049365	36.0	36.0	36.0	36.0	36.0
8	35.02353407031937	36.0	36.0	36.0	36.0	36.0
9	35.145693020002845	36.0	36.0	36.0	36.0	36.0
10	34.98362951623935	36.0	36.0	36.0	36.0	36.0
11	35.136905241468924	36.0	36.0	36.0	36.0	36.0
12	35.03795038114943	36.0	36.0	36.0	36.0	36.0
13	35.08682784593575	36.0	36.0	36.0	36.0	36.0
14	35.01885091203307	36.0	36.0	36.0	36.0	36.0
15	34.9893386153142	36.0	36.0	36.0	36.0	36.0
16	35.004887146513255	36.0	36.0	36.0	36.0	36.0
17	34.964769176178976	36.0	36.0	36.0	36.0	36.0
18	34.95950490857385	36.0	36.0	36.0	36.0	36.0
19	34.96076740713821	36.0	36.0	36.0	36.0	36.0
20	34.950989685738136	36.0	36.0	36.0	36.0	36.0
21	34.945257445141735	36.0	36.0	36.0	36.0	36.0
22	34.93261617472363	36.0	36.0	36.0	36.0	36.0
23	34.875245771529755	36.0	36.0	36.0	32.0	36.0
24	34.84574461702496	36.0	36.0	36.0	32.0	36.0
25	34.80952813580473	36.0	36.0	36.0	32.0	36.0
26	34.75142234648172	36.0	36.0	36.0	32.0	36.0
27	34.75012984964868	36.0	36.0	36.0	32.0	36.0
28	34.7185365301774	36.0	36.0	36.0	32.0	36.0
29	34.688319702691444	36.0	36.0	36.0	32.0	36.0
30	34.661193553972026	36.0	36.0	36.0	32.0	36.0
31	34.659641357841636	36.0	36.0	36.0	32.0	36.0
32	34.61304804694129	36.0	36.0	36.0	32.0	36.0
33	34.586729280195826	36.0	36.0	36.0	32.0	36.0
34	34.565313087644654	36.0	36.0	36.0	32.0	36.0
35	34.53158903400432	36.0	36.0	36.0	32.0	36.0
36	34.49249957573877	36.0	36.0	36.0	32.0	36.0
37	34.47958403543567	36.0	36.0	36.0	32.0	36.0
38	34.429167230919816	36.0	36.0	36.0	32.0	36.0
39	34.41046459604331	36.0	36.0	36.0	32.0	36.0
40	34.38844329555837	36.0	36.0	36.0	32.0	36.0
41	34.366152867748774	36.0	36.0	36.0	32.0	36.0
42	34.28999240615256	36.0	36.0	36.0	32.0	36.0
43	34.28431844790672	36.0	36.0	36.0	32.0	36.0
44	34.20679263653926	36.0	36.0	36.0	32.0	36.0
45	34.17667437479323	36.0	36.0	36.0	32.0	36.0
46	34.12450995685392	36.0	36.0	36.0	32.0	36.0
47	34.09407714183353	36.0	36.0	36.0	32.0	36.0
48	34.040264533304075	36.0	36.0	36.0	32.0	36.0
49	34.00286097773785	36.0	36.0	36.0	32.0	36.0
50	33.40219964447766	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	5.0
20	5.0
21	35.0
22	144.0
23	382.0
24	1050.0
25	2395.0
26	4792.0
27	8658.0
28	14322.0
29	22243.0
30	31420.0
31	44914.0
32	64799.0
33	103413.0
34	211533.0
35	656622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.08899213111381	17.856885703999588	11.534261370454818	26.519860794431775
2	16.112752929560276	20.232393497027584	36.600732990037095	27.054120583375045
3	18.489399414430785	24.153530214411585	28.22037355750441	29.136696813653224
4	12.19275344680107	15.399825495785672	35.36178769111039	37.04563336630286
5	14.46199390778018	36.896584825675774	32.86798876179146	15.773432504752583
6	35.16523074707816	35.20122873119105	16.20003565514617	13.433504866584615
7	30.85296220046729	30.054751125792166	20.435249165619584	18.65703750812096
8	28.63266177209201	32.778508211811776	18.93319299857551	19.655637017520704
9	27.760816648381788	13.673546352166758	18.16305540318157	40.40258159626989
10	16.082292379135318	26.450093338750236	31.10503137861934	26.362582903495102
11	38.068402909317804	20.53921459390058	22.213289404440086	19.179093092341528
12	25.332638515100296	23.213471474168877	28.48563337595952	22.96825663477131
13	29.87270339857584	19.0548921595853	25.258244451986933	25.814159989851927
14	23.760832616944267	19.48915388380012	24.922025728295555	31.82798777096006
15	25.3547226931668	27.50680131586391	21.864494816943033	25.27398117402626
16	25.994363715216767	25.241316298771093	23.93552556469985	24.828794421312285
17	23.987472723002845	25.83399472373309	25.065353371034014	25.113179182230056
18	25.06796785540417	24.298294032542287	26.610743892321086	24.02299421973245
19	25.990242848841294	24.20740288703338	25.296168621125293	24.506185643000034
20	26.187360025095845	23.76391392330854	24.85834303997422	25.1903830116214
21	27.268169094240847	23.67103384318962	24.15623441161396	24.904562650955572
22	26.221531594230722	24.041339399279355	24.836294881772336	24.900834124717587
23	24.542030041355076	23.92980350982451	25.601319934003296	25.926846514817115
24	25.178619181163047	24.5135112896922	25.267071673946283	25.04079785519847
25	25.09203237482526	24.178779798561596	25.48296104684201	25.246226779771135
26	24.709425914751222	25.24111649020791	25.79003579461298	24.259421800427887
27	25.38507428912329	24.419409972759798	25.025157329740168	25.170358408376746
28	24.64741529309916	24.53170133799618	25.62035537041323	25.200527998491435
29	24.58095339317183	24.380644143804382	25.595185035347683	25.443217427676103
30	24.383892755511365	24.8530016799808	25.92578941954949	24.837316144958344
31	25.473753685798535	24.5211033395049	24.662106562435714	25.34303641226085
32	24.876402260072958	24.777061219451987	24.607950026056667	25.738586494418385
33	24.429598887097477	24.37928513327145	25.4541309134732	25.736985066157875
34	25.40616940360489	24.43239138316592	25.451596949668847	24.709842263560336
35	25.843819561168402	24.26242126222155	25.56833912036541	24.32542005624464
36	24.48188904565411	25.10418791711596	25.113616687895686	25.300306349334246
37	25.52846629489885	24.801787639777213	24.76115946302236	24.90858660230158
38	24.745422752520057	24.731279572104505	25.246948501680038	25.2763491736954
39	25.65669587219738	24.361989724636015	24.5611884725296	25.420125930637006
40	25.912485086191527	24.718437581425967	25.049884117993937	24.31919321438857
41	24.70405617254269	25.122761725255444	25.642350888110048	24.53083121409182
42	26.10100573752085	25.38368706426469	24.791764163379636	23.723543034834826
43	25.00064282885839	24.36089954899127	25.35145596450899	25.28700165764135
44	24.624864255158304	25.16115816170414	24.69197599062664	25.52200159251091
45	24.671557871640154	25.37121160175535	24.780924300603402	25.1763062260011
46	24.668300877674163	24.779552934722986	24.988171969281403	25.56397421832145
47	25.216358289498835	24.55474805412536	25.187301951917334	25.041591704458476
48	25.802127349555587	25.282203803858717	24.02362197975504	24.89204686683066
49	24.81454594075241	25.46131027631905	24.4211390629031	25.30300472002544
50	24.80625204633152	25.751635779560168	24.268420412060458	25.173691762047856
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	31.5
2	31.0
3	96.0
4	161.0
5	188.5
6	216.0
7	224.5
8	233.0
9	302.0
10	371.0
11	582.0
12	793.0
13	1483.5
14	2174.0
15	2827.0
16	3480.0
17	3830.5
18	4181.0
19	4291.0
20	4401.0
21	4735.5
22	5070.0
23	5649.5
24	6229.0
25	6489.5
26	6750.0
27	8453.0
28	10156.0
29	11675.5
30	13195.0
31	15389.0
32	17583.0
33	21147.0
34	24711.0
35	28766.0
36	32821.0
37	38106.0
38	43391.0
39	45901.0
40	48411.0
41	52331.5
42	56252.0
43	57040.5
44	57829.0
45	64003.0
46	70177.0
47	75481.5
48	80786.0
49	83974.5
50	87163.0
51	84621.0
52	82079.0
53	81949.0
54	81819.0
55	83469.0
56	85119.0
57	83848.0
58	82577.0
59	76207.0
60	69837.0
61	61914.5
62	53992.0
63	47460.5
64	40929.0
65	34817.0
66	28705.0
67	24889.0
68	21073.0
69	19029.0
70	16985.0
71	13185.5
72	9386.0
73	8062.5
74	6739.0
75	5186.0
76	3633.0
77	3226.0
78	2819.0
79	2339.5
80	1860.0
81	1527.0
82	1194.0
83	976.0
84	758.0
85	534.0
86	310.0
87	215.5
88	121.0
89	101.0
90	81.0
91	60.0
92	39.0
93	31.0
94	23.0
95	27.5
96	32.0
97	23.5
98	15.0
99	13.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009770864652954315
2	5.142560343660166E-4
3	5.142560343660166E-4
4	0.0
5	0.0
6	1.7141867812200555E-4
7	0.0
8	0.0
9	0.0011999307468540388
10	0.0013713494249760444
11	0.0
12	1.7141867812200555E-4
13	8.570933906100277E-4
14	2.571280171830083E-4
15	0.004114048274928133
16	6.856747124880222E-4
17	0.0
18	5.142560343660166E-4
19	0.0
20	5.999653734270194E-4
21	0.0
22	1.7141867812200555E-4
23	7.713840515490249E-4
24	5.142560343660166E-4
25	0.002142733476525069
26	0.006685328446758216
27	0.00651390976863621
28	0.0054853976999041775
29	0.003256954884318105
30	0.004628304309294149
31	0.008056677871734261
32	0.003942629596806127
33	0.004714013648355152
34	0.0035140829015011133
35	0.003856920257745124
36	0.007885259193612253
37	0.004799722987416155
38	0.008056677871734261
39	0.005142560343660166
40	0.002571280171830083
41	0.003685501579623119
42	0.0023998614937080776
43	0.0013713494249760444
44	0.0019713147984030633
45	0.0011999307468540388
46	0.0011999307468540388
47	0.002999826867135097
48	0.0020570241374640664
49	2.571280171830083E-4
50	0.0010285120687320332
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1166734.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.852222302380284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.90524513644161	42.184236024431456
2	13.718807764270238	15.050141864187438
3	4.457151914318041	7.3345406291895925
4	1.8458678550877756	4.049998157123702
5	0.8921068218215175	2.446702085401192
6	0.5296736739301589	1.7432266866081336
7	0.3221792206520355	1.2370572362689158
8	0.23097579706588775	1.013562861370204
9	0.16691446412446972	0.8240066362474284
>10	0.7663832839505277	7.451247318965058
>50	0.07521286820197033	2.864926489798104
>100	0.07910201326974964	9.168665107784117
>500	0.008334801574197507	3.218736538766689
>1k	0.002044385291784294	1.4129523638580255
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1490	0.12770691520089414	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1421	0.12179297080568492	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1410	0.12085016807601391	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1389	0.11905027195573284	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1324	0.11347916491676767	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1263	0.1082508952340465	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1250	0.10713667382625344	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1229	0.1053367777059724	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1190	0.10199411348259328	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.570933906100276E-5
2	0.0	0.0	0.0	1.7141867812200552E-4	8.570933906100276E-5
3	0.0	0.0	0.0	1.7141867812200552E-4	8.570933906100276E-5
4	0.0	0.0	0.0	2.571280171830083E-4	8.570933906100276E-5
5	0.0	0.0	0.0	2.571280171830083E-4	8.570933906100276E-5
6	0.0	0.0	0.0	2.571280171830083E-4	8.570933906100276E-5
7	0.0	0.0	0.0	4.2854669530501384E-4	8.570933906100276E-5
8	0.0	0.0	0.0	4.2854669530501384E-4	1.7141867812200552E-4
9	0.0	0.0	0.0	4.2854669530501384E-4	1.7141867812200552E-4
10	0.0	0.0	0.0	4.2854669530501384E-4	1.7141867812200552E-4
11	0.0	0.0	0.0	4.2854669530501384E-4	1.7141867812200552E-4
12	0.0	0.0	0.0	4.2854669530501384E-4	4.2854669530501384E-4
13	0.0	0.0	0.0	4.2854669530501384E-4	5.999653734270194E-4
14	0.0	0.0	0.0	4.2854669530501384E-4	5.999653734270194E-4
15	0.0	0.0	0.0	4.2854669530501384E-4	6.856747124880221E-4
16	0.0	0.0	0.0	6.856747124880221E-4	6.856747124880221E-4
17	0.0	0.0	0.0	6.856747124880221E-4	6.856747124880221E-4
18	0.0	0.0	0.0	7.713840515490249E-4	6.856747124880221E-4
19	0.0	0.0	0.0	9.428027296710304E-4	6.856747124880221E-4
20	0.0	0.0	0.0	0.001114221407793036	7.713840515490249E-4
21	0.0	0.0	0.0	0.0011999307468540388	7.713840515490249E-4
22	0.0	0.0	0.0	0.0019713147984030638	7.713840515490249E-4
23	0.0	0.0	0.0	0.0033426642233791077	7.713840515490249E-4
24	0.0	0.0	0.0	0.004542594970233147	7.713840515490249E-4
25	0.0	0.0	0.0	0.005485397699904177	7.713840515490249E-4
26	0.0	0.0	0.0	0.006171072412392199	7.713840515490249E-4
27	0.0	0.0	0.0	0.007885259193612255	8.570933906100277E-4
28	0.0	0.0	0.0	0.011827888790418382	8.570933906100277E-4
29	0.0	0.0	0.0	0.019027473271542614	8.570933906100277E-4
30	0.0	0.0	0.0	0.0339408982681571	8.570933906100277E-4
31	0.0	0.0	0.0	0.060682212055189956	8.570933906100277E-4
32	0.0	0.0	0.0	0.08948054997968688	8.570933906100277E-4
33	0.0	0.0	0.0	0.1146790956636217	8.570933906100277E-4
34	0.0	0.0	0.0	0.14476307367403368	9.428027296710304E-4
35	0.0	0.0	0.0	0.18204663616556988	9.428027296710304E-4
36	0.0	0.0	0.0	0.2358721010958796	9.428027296710304E-4
37	0.0	0.0	0.0	0.3045252816837428	9.428027296710304E-4
38	0.0	0.0	0.0	0.40094828812737093	0.0010285120687320332
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	40	7.0329465E-4	27.499357	5
TATACTG	150	0.0	23.466118	5
GTATGCG	70	3.2168733E-5	21.998543	11
GATATAC	265	0.0	21.58625	1
TAGGACG	410	0.0	20.92634	4
TTAGGAC	315	0.0	20.253494	3
ACCGTAT	55	0.0044841254	19.999533	8
GACGTGA	295	0.0	19.389378	7
GTCGTGT	70	8.1196864E-4	18.858318	1
GGACGTG	470	0.0	18.722967	6
GTATCAA	2905	0.0	18.403902	1
AGGACGT	455	0.0	18.373198	5
GTCCTAC	515	0.0	18.370077	1
TTATACT	170	1.2732926E-10	18.117224	4
TATGCGA	75	0.0012914069	17.598835	12
CCCTCGA	390	0.0	17.48827	26
GATTTCG	205	5.456968E-12	17.17033	41
AACGCAG	3105	0.0	17.146137	6
TCCTACA	565	0.0	17.132343	2
GTGTACG	90	2.2142331E-4	17.112179	1
>>END_MODULE