FastQCFastQC Report
Thu 2 Feb 2017
SRR4064020_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064020_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5968524
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT134100.22467866427277497No Hit
TATCAACGCAGAGTACTTTTTTTTT86340.14465888048703499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30600.014.0597851
GTATCAA86050.012.8360311
GGACCGT2750.012.4359036
CGACCAT9600.012.17264810
AAGACGG10500.012.0319795
GCGTTAT4650.011.64191
CGTTATT4250.011.6180332
GCGTAAC5850.011.53045411
CCGACCA10200.011.3633729
CAAGACG10950.011.3631614
CGCATCG7000.010.99374113
TAACGAA5200.010.96241913
CGCCAGT7500.010.64210418
GACCATA11500.010.49179611
CGCGTAA6650.010.429234510
CGAACGA5400.010.38150916
CGGTCCA9000.010.34510110
CCGTCGT8850.010.3056539
CGATAAC5350.010.29972110
CGGACCA11200.010.2639589
Produced by FastQC (version 0.11.5)