##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064020_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5968524
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244247321448317	32.0	32.0	32.0	32.0	32.0
2	30.747086381825724	32.0	32.0	32.0	32.0	32.0
3	30.77614951368211	32.0	32.0	32.0	32.0	32.0
4	30.810831120055813	32.0	32.0	32.0	32.0	32.0
5	30.664903081565896	32.0	32.0	32.0	32.0	32.0
6	34.36405466410121	36.0	36.0	36.0	32.0	36.0
7	34.295161416792496	36.0	36.0	36.0	32.0	36.0
8	34.242414875101446	36.0	36.0	36.0	32.0	36.0
9	34.438084859841396	36.0	36.0	36.0	32.0	36.0
10	34.04614323407261	36.0	36.0	36.0	32.0	36.0
11	34.41697260495225	36.0	36.0	36.0	32.0	36.0
12	34.17713156552608	36.0	36.0	36.0	32.0	36.0
13	34.29520883220039	36.0	36.0	36.0	32.0	36.0
14	34.16371484809309	36.0	36.0	36.0	32.0	36.0
15	34.082740556961824	36.0	36.0	36.0	32.0	36.0
16	34.08564077148722	36.0	36.0	36.0	32.0	36.0
17	34.00133299288065	36.0	36.0	36.0	32.0	36.0
18	33.997952425088684	36.0	36.0	36.0	32.0	36.0
19	34.02949506444139	36.0	36.0	36.0	32.0	36.0
20	34.01442081827936	36.0	36.0	36.0	32.0	36.0
21	34.003376044060474	36.0	36.0	36.0	32.0	36.0
22	33.9784536344329	36.0	36.0	36.0	32.0	36.0
23	33.90659583508418	36.0	36.0	36.0	32.0	36.0
24	33.89050090106029	36.0	36.0	36.0	32.0	36.0
25	33.46694660187343	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	21.0
4	47.0
5	65.0
6	260.0
7	95.0
8	337.0
9	418.0
10	552.0
11	159.0
12	368.0
13	319.0
14	507.0
15	984.0
16	1594.0
17	2131.0
18	2889.0
19	3913.0
20	6170.0
21	9325.0
22	14860.0
23	23569.0
24	34927.0
25	51158.0
26	72264.0
27	95286.0
28	129152.0
29	173002.0
30	226936.0
31	307131.0
32	430317.0
33	604766.0
34	1256483.0
35	2518517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.275738233469134	17.152419579491273	11.573225311513086	25.99861687552651
2	16.822417065907445	19.239710847637053	37.51107326610707	26.426798820348434
3	18.690117017745596	23.14292698676415	28.670662928541617	29.496293066948642
4	12.525243826080196	15.371871110172075	36.01057656203426	36.09230850171347
5	14.588712389474692	36.40486550132482	33.74835392294575	15.25806818625474
6	34.31152754571822	35.65420630437504	16.696625180534465	13.337640969372272
7	30.690873282612124	30.471739424436777	20.31377233507102	18.52361495788008
8	28.41374675157613	32.774013614677635	19.033530522404384	19.77870911134185
9	27.409270325574287	14.063713477598297	18.351615802127917	40.1754003946995
10	15.62863730220159	26.521327970808876	31.587092993060807	26.26294173392873
11	37.168245291492525	21.139831888651756	22.422837206316018	19.2690856135397
12	24.57235679017665	23.60438289967001	28.635157987951505	23.188102322201836
13	29.2213702575761	19.52922596399283	25.371065671133824	25.87833810729725
14	23.667913656424002	19.42093356740728	25.23250493713049	31.678647839038227
15	25.40844350726794	27.098999121872026	22.147198925994836	25.345358444865195
16	25.92036530433673	25.82454936750584	23.422159652655075	24.832925675502356
17	24.075458134120893	25.93783666608659	24.95085282874118	25.035852371051337
18	24.662478139341474	25.099087679056193	25.87490421552304	24.3635299660793
19	25.508865630835437	24.90324188112907	25.453350120445688	24.134542367589805
20	25.854197393493187	24.224929424713324	24.958248404887854	24.962624776905635
21	26.96327120681997	24.105245914118566	24.52878623839351	24.402696640667955
22	25.972460485788833	24.018208320205662	25.048110563163128	24.96122063084237
23	24.453670757023417	24.154389448019828	25.495638555105792	25.89630123985096
24	24.545225134287467	24.941309230476445	25.328674177989686	25.18479145724641
25	24.854276302827618	24.413353443368425	25.38391694633756	25.348453307466396
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	269.0
1	269.0
2	840.0
3	1411.0
4	1411.0
5	1411.0
6	3731.5
7	6052.0
8	6052.0
9	6052.0
10	7392.5
11	8733.0
12	8733.0
13	8733.0
14	12611.0
15	16489.0
16	16489.0
17	16489.0
18	29277.0
19	42065.0
20	42065.0
21	42065.0
22	65129.0
23	88193.0
24	88193.0
25	88193.0
26	127240.5
27	166288.0
28	166288.0
29	166288.0
30	206189.0
31	246090.0
32	246090.0
33	246090.0
34	306330.5
35	366571.0
36	366571.0
37	366571.0
38	424422.5
39	482274.0
40	482274.0
41	482274.0
42	554267.5
43	626261.0
44	626261.0
45	626261.0
46	694726.5
47	763192.0
48	763192.0
49	763192.0
50	789410.0
51	815628.0
52	815628.0
53	815628.0
54	763810.5
55	711993.0
56	711993.0
57	711993.0
58	652618.0
59	593243.0
60	593243.0
61	593243.0
62	518819.5
63	444396.0
64	444396.0
65	444396.0
66	366540.5
67	288685.0
68	288685.0
69	288685.0
70	217153.5
71	145622.0
72	145622.0
73	145622.0
74	111976.5
75	78331.0
76	78331.0
77	78331.0
78	61011.0
79	43691.0
80	43691.0
81	43691.0
82	29982.0
83	16273.0
84	16273.0
85	16273.0
86	12405.0
87	8537.0
88	8537.0
89	8537.0
90	6087.5
91	3638.0
92	3638.0
93	3638.0
94	2565.0
95	1492.0
96	1492.0
97	1492.0
98	2299.5
99	3107.0
100	3107.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038904090860654994
2	0.0053782141112274995
3	0.0018932654036408333
4	0.003903812734940833
5	0.009164744918509165
6	0.013856022024875831
7	0.0233726127263625
8	0.0363406430132475
9	0.0498113101329575
10	0.0573508626253325
11	0.05547435178278583
12	0.06361706847455083
13	0.0649909424842725
14	0.06747061752620916
15	0.059679746617421654
16	0.0663983256161825
17	0.063432768302515
18	0.07730554488848498
19	0.07413893284168749
20	0.0784113459207
21	0.07557982509578583
22	0.07802599101553416
23	0.08216436760579332
24	0.07772440891583916
25	0.07822704574866417
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5968524.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.55994302188614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.889921757334065	17.39012695996566
2	15.38549173882265	8.788175348489496
3	7.532001478304781	6.453405991834401
4	4.321187990011734	4.9365153112637525
5	2.718348525034129	3.881793949430148
6	1.7971978769586072	3.079672137899554
7	1.3052232797563292	2.6093971750476084
8	0.9094732226340058	2.07796027346867
9	0.7179260699505721	1.8453534886543481
>10	3.862160565393084	20.029985017124048
>50	0.32931938790867843	6.537046725523147
>100	0.19991573641633065	10.604399387818429
>500	0.01803835090628134	3.546772154135865
>1k	0.013263481316279238	7.233393816425069
>5k	4.715904467997252E-4	0.7602349176040633
>10k+	5.894880584996565E-5	0.22576734531558348
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	13410	0.22467866427277497	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8634	0.14465888048703499	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.0263683282499994E-5
2	0.0	0.0	0.0	0.0	5.0263683282499994E-5
3	0.0	0.0	0.0	0.0	5.0263683282499994E-5
4	0.0	0.0	0.0	0.0	5.0263683282499994E-5
5	0.0	0.0	0.0	0.0	5.0263683282499994E-5
6	0.0	0.0	0.0	0.0	6.701824437666666E-5
7	0.0	0.0	0.0	1.6754561094166666E-5	6.701824437666666E-5
8	0.0	0.0	0.0	1.6754561094166666E-5	6.701824437666666E-5
9	0.0	0.0	0.0	1.6754561094166666E-5	6.701824437666666E-5
10	0.0	0.0	0.0	3.350912218833333E-5	6.701824437666666E-5
11	0.0	0.0	0.0	3.350912218833333E-5	6.701824437666666E-5
12	0.0	0.0	0.0	3.350912218833333E-5	2.1780929422416664E-4
13	0.0	0.0	0.0	3.350912218833333E-5	3.183366607891666E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3060	0.0	14.059785	1
GTATCAA	8605	0.0	12.836031	1
GGACCGT	275	0.0	12.435903	6
CGACCAT	960	0.0	12.172648	10
AAGACGG	1050	0.0	12.031979	5
GCGTTAT	465	0.0	11.6419	1
CGTTATT	425	0.0	11.618033	2
GCGTAAC	585	0.0	11.530454	11
CCGACCA	1020	0.0	11.363372	9
CAAGACG	1095	0.0	11.363161	4
CGCATCG	700	0.0	10.993741	13
TAACGAA	520	0.0	10.962419	13
CGCCAGT	750	0.0	10.642104	18
GACCATA	1150	0.0	10.491796	11
CGCGTAA	665	0.0	10.4292345	10
CGAACGA	540	0.0	10.381509	16
CGGTCCA	900	0.0	10.345101	10
CCGTCGT	885	0.0	10.305653	9
CGATAAC	535	0.0	10.299721	10
CGGACCA	1120	0.0	10.263958	9
>>END_MODULE