FastQCFastQC Report
Thu 2 Feb 2017
SRR4064020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5968524
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86850.028.041591
GGTATCA36700.026.256261
CGCAATA15200.019.10599736
CGTCGTA15550.018.81602510
ACCGTCG16050.018.3667688
ATACCGT16850.018.1473946
TCAACGC132550.018.1401044
TACCGTC17400.018.0794897
TATCAAC137250.017.8712372
CCGTCGT16300.017.8154439
ATCAACG136150.017.692773
CAACGCA136300.017.657165
AACGCAG137100.017.5541276
CTAGCGG16350.017.49361429
ATACGAA17050.016.90274440
TAGCGGC17400.016.4382430
TCTAGCG17750.016.2377928
CGAATGC18100.015.80078643
CAATACG18150.015.63657238
AATACGA18650.015.45277139
Produced by FastQC (version 0.11.5)