##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064020_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5968524
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.318188047832262	32.0	32.0	32.0	32.0	32.0
2	31.418241092772686	32.0	32.0	32.0	32.0	32.0
3	31.498120473336456	32.0	32.0	32.0	32.0	32.0
4	31.58241082719949	32.0	32.0	32.0	32.0	32.0
5	31.531469254375118	32.0	32.0	32.0	32.0	32.0
6	35.12895466282786	36.0	36.0	36.0	36.0	36.0
7	35.144738967289065	36.0	36.0	36.0	36.0	36.0
8	35.09105886145385	36.0	36.0	36.0	36.0	36.0
9	35.20343153516682	36.0	36.0	36.0	36.0	36.0
10	35.05823332535816	36.0	36.0	36.0	36.0	36.0
11	35.19886239881083	36.0	36.0	36.0	36.0	36.0
12	35.10523456050441	36.0	36.0	36.0	36.0	36.0
13	35.14496331086212	36.0	36.0	36.0	36.0	36.0
14	35.09101613732307	36.0	36.0	36.0	36.0	36.0
15	35.06680211053855	36.0	36.0	36.0	36.0	36.0
16	35.0785338217623	36.0	36.0	36.0	36.0	36.0
17	35.041407389833736	36.0	36.0	36.0	36.0	36.0
18	35.03741863147405	36.0	36.0	36.0	36.0	36.0
19	35.037424160479205	36.0	36.0	36.0	36.0	36.0
20	35.030943998884815	36.0	36.0	36.0	36.0	36.0
21	35.02380286985526	36.0	36.0	36.0	36.0	36.0
22	35.01011958735526	36.0	36.0	36.0	36.0	36.0
23	34.959932975053796	36.0	36.0	36.0	36.0	36.0
24	34.931151487369405	36.0	36.0	36.0	36.0	36.0
25	34.90762389495292	36.0	36.0	36.0	32.0	36.0
26	34.847680934180715	36.0	36.0	36.0	32.0	36.0
27	34.84172602807662	36.0	36.0	36.0	32.0	36.0
28	34.826916336434266	36.0	36.0	36.0	32.0	36.0
29	34.795861254809395	36.0	36.0	36.0	32.0	36.0
30	34.7812299992427	36.0	36.0	36.0	32.0	36.0
31	34.77387793028896	36.0	36.0	36.0	32.0	36.0
32	34.742534670213274	36.0	36.0	36.0	32.0	36.0
33	34.71816683655791	36.0	36.0	36.0	32.0	36.0
34	34.714681385213495	36.0	36.0	36.0	32.0	36.0
35	34.67773925345697	36.0	36.0	36.0	32.0	36.0
36	34.65155019901068	36.0	36.0	36.0	32.0	36.0
37	34.64596205024894	36.0	36.0	36.0	32.0	36.0
38	34.6070735411301	36.0	36.0	36.0	32.0	36.0
39	34.6099921856727	36.0	36.0	36.0	32.0	36.0
40	34.5875713995621	36.0	36.0	36.0	32.0	36.0
41	34.57571587213187	36.0	36.0	36.0	32.0	36.0
42	34.51305800227996	36.0	36.0	36.0	32.0	36.0
43	34.5016798122953	36.0	36.0	36.0	32.0	36.0
44	34.44681918008539	36.0	36.0	36.0	32.0	36.0
45	34.42203700613418	36.0	36.0	36.0	32.0	36.0
46	34.39966112224731	36.0	36.0	36.0	32.0	36.0
47	34.37719610409542	36.0	36.0	36.0	32.0	36.0
48	34.33346418645548	36.0	36.0	36.0	32.0	36.0
49	34.31770183046931	36.0	36.0	36.0	32.0	36.0
50	33.7296036675064	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	6.0
18	9.0
19	20.0
20	60.0
21	231.0
22	672.0
23	1885.0
24	4833.0
25	10922.0
26	22178.0
27	39365.0
28	64518.0
29	99383.0
30	142725.0
31	203382.0
32	292271.0
33	471960.0
34	995471.0
35	3618632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30144482698587	18.06409906465855	12.091576699054505	26.54287940930107
2	16.05718892759954	19.897836943230942	37.0416309094851	27.003343219684417
3	17.935993038124945	23.687515152449347	28.986629319526642	29.389862489899066
4	12.160929569856801	15.978020696574227	36.05390545468193	35.807144278887044
5	14.157989479475997	36.84812191422871	33.53458912119646	15.459299485098828
6	34.31105871764039	36.021786331034214	16.45393081995465	13.213224131370746
7	30.556200141509187	30.59672947232956	20.302899363443213	18.54417102271804
8	28.37353563017182	32.95319461783091	18.89877948028348	19.77449027171379
9	27.36281914258184	13.919704151142684	18.25282028693197	40.464656419343505
10	15.48514389651193	26.754222532594696	31.627710751323292	26.132922819570087
11	37.33317271291406	21.09270585034187	22.36358978594872	19.21053165079535
12	24.514477137860446	23.679981315282163	28.624914007071105	23.180627539786286
13	29.237871140649936	19.510238096993344	25.38220709686802	25.8696836654887
14	23.657185346823695	19.34098102909471	25.235083876908597	31.766749747172994
15	25.38279808789452	27.159906773967084	22.083042211420693	25.374252926717705
16	25.98785383655339	25.831180492015626	23.37302916376042	24.80793650767056
17	24.13129276182855	25.986558820907817	24.9192932792094	24.962855138054234
18	24.706358775090862	25.0859008650068	25.892936727235682	24.314803632666656
19	25.535509281691755	24.90168423549943	25.41745664422226	24.145349838586558
20	25.943051095313656	24.165996897363865	24.954355996919144	24.93659601040333
21	26.969146810836314	24.169912025150605	24.523684582653935	24.337256581359142
22	25.9484521607891	24.07079886216234	25.069891768704906	24.91085720834365
23	24.490455848666123	24.070566134849926	25.51527426115093	25.923703755333023
24	24.580434431125433	24.891668670242666	25.340240713948823	25.18765618468307
25	24.875293628799582	24.299513103970565	25.513569755479676	25.31162351175018
26	24.739909322850227	24.990068206414424	25.529811549766958	24.74021092096839
27	25.256147515232126	24.788078971968332	24.90573701065809	25.050036502141452
28	24.690723394102356	24.518194683160075	25.69580324790283	25.095278674834738
29	24.6151381883447	24.786843188163747	25.54064958373936	25.057369039752185
30	24.639666289506522	24.940874994784938	25.536640580454662	24.882818135253885
31	25.2009771970597	24.583576990293377	24.874842705120066	25.340603107526867
32	25.040228914419142	24.743027546669563	24.65157839427486	25.56516514463644
33	24.671218311041468	24.448623598960232	25.19857593001361	25.681582159984696
34	25.202003032328363	24.599022410042572	25.44888864612688	24.75008591150219
35	25.719362020687814	24.30892053139028	25.369834580580473	24.601882867341427
36	24.639250866319028	24.905115844041294	25.122908305151398	25.332724984488276
37	25.696426097043158	24.60626514147345	24.87780006779158	24.819508693691812
38	24.943985110059288	24.269762214027484	25.34607584111141	25.440176834801818
39	25.384480439740365	24.569149175914074	24.75711050259465	25.289259881750915
40	25.506269389735614	24.684050771702264	25.124841581378078	24.684838257184047
41	24.556888726773362	25.03983959275567	25.573407811157228	24.829863869313737
42	25.773421289668818	24.774680585267514	25.161533646448238	24.29036447861543
43	25.179758117577517	24.00082299603732	25.40025135558941	25.41916753079575
44	24.652049002411182	24.684369000212953	25.172084301411374	25.491497695964487
45	24.99253573548054	24.853102268968865	25.061029368152944	25.09333262739765
46	24.742559262764726	24.643639289927506	25.10846598478234	25.50533546252543
47	24.811938616013645	24.625456383012054	25.576616300825833	24.985988700148464
48	25.592246257427213	25.035248016183846	24.633131610521442	24.739374115867495
49	24.83241188492523	24.996104550186963	24.984326050322174	25.187157514565634
50	24.813958768085772	25.224619184090972	24.823374971830976	25.138047075992283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	95.0
2	149.0
3	377.5
4	606.0
5	770.5
6	935.0
7	864.5
8	794.0
9	1080.5
10	1367.0
11	2079.0
12	2791.0
13	5343.0
14	7895.0
15	10223.5
16	12552.0
17	12681.5
18	12811.0
19	12798.5
20	12786.0
21	15190.0
22	17594.0
23	21001.5
24	24409.0
25	31575.0
26	38741.0
27	47122.5
28	55504.0
29	64801.0
30	74098.0
31	83807.5
32	93517.0
33	105255.5
34	116994.0
35	136326.5
36	155659.0
37	182625.0
38	209591.0
39	234669.0
40	259747.0
41	284217.5
42	308688.0
43	318858.0
44	329028.0
45	357867.5
46	386707.0
47	414597.0
48	442487.0
49	456097.0
50	469707.0
51	460397.0
52	451087.0
53	446337.5
54	441588.0
55	439932.0
56	438276.0
57	422499.0
58	406722.0
59	372542.5
60	338363.0
61	296626.5
62	254890.0
63	223273.5
64	191657.0
65	161511.5
66	131366.0
67	113244.0
68	95122.0
69	85006.5
70	74891.0
71	57485.5
72	40080.0
73	33897.0
74	27714.0
75	21339.0
76	14964.0
77	12831.5
78	10699.0
79	8837.0
80	6975.0
81	5473.5
82	3972.0
83	3296.0
84	2620.0
85	1834.0
86	1048.0
87	768.5
88	489.0
89	369.0
90	249.0
91	207.5
92	166.0
93	141.5
94	117.0
95	122.0
96	127.0
97	98.0
98	69.0
99	72.0
100	75.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009097726674132499
2	4.356185884483333E-4
3	2.848275386008333E-4
4	0.0
5	0.0
6	1.843001720358333E-4
7	1.1728192765916665E-4
8	1.6754561094166666E-5
9	0.0011895738376858332
10	0.0011393101544033332
11	1.6754561094166666E-5
12	1.6754561094166665E-4
13	5.361459550133333E-4
14	2.6807297750666665E-4
15	0.003920567296035
16	9.215008601791665E-4
17	0.0
18	2.6807297750666665E-4
19	0.0
20	8.544826158024999E-4
21	0.0
22	1.6754561094166665E-4
23	8.712371768966666E-4
24	4.858822717308333E-4
25	0.001742474353793333
26	0.005311195866850833
27	0.006383487776877499
28	0.004054603784788333
29	0.0024796750419366666
30	0.003970830979317499
31	0.007271479514868333
32	0.0030158209969499998
33	0.004657767984178333
34	0.002898539069290833
35	0.0030158209969499998
36	0.007656834420034165
37	0.0038703036127524996
38	0.00784113459207
39	0.004607504300895833
40	0.0026807297750666664
41	0.003602230635245833
42	0.0018094925981699999
43	0.0014576468151924998
44	0.001390628570815833
45	0.0014408922540983331
46	0.0010555373489325
47	0.002127829258959166
48	0.0017257197926991664
49	3.686003440716666E-4
50	0.0014911559373808332
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5968524.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.014883290515638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.097899112576314	19.259590935627692
2	15.359623703823996	9.527538731206775
3	7.153070561184584	6.655549458718892
4	4.127774526744629	5.120897807861924
5	2.700619671562268	4.187970196278721
6	1.7506562534415242	3.257783962938014
7	1.2377666658150672	2.6872432076801487
8	0.9107369820668709	2.259712096572835
9	0.6393322185125428	1.784593272692966
>10	3.5403128087611995	19.68408282604933
>50	0.28730462705751725	6.187384915361565
>100	0.16923110326116342	9.651229592755831
>500	0.015065855728124264	3.2076854943518307
>1k	0.010333963067786889	6.096492101023021
>5k	2.7194639651881677E-4	0.4322454008805051
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.0263683282499994E-5
2	0.0	0.0	0.0	0.0	5.0263683282499994E-5
3	0.0	0.0	0.0	0.0	5.0263683282499994E-5
4	0.0	0.0	0.0	0.0	5.0263683282499994E-5
5	1.6754561094166666E-5	0.0	0.0	0.0	5.0263683282499994E-5
6	1.6754561094166666E-5	0.0	0.0	0.0	5.0263683282499994E-5
7	1.6754561094166666E-5	0.0	0.0	0.0	5.0263683282499994E-5
8	1.6754561094166666E-5	0.0	0.0	1.6754561094166666E-5	5.0263683282499994E-5
9	1.6754561094166666E-5	0.0	0.0	1.6754561094166666E-5	5.0263683282499994E-5
10	1.6754561094166666E-5	0.0	0.0	3.350912218833333E-5	5.0263683282499994E-5
11	1.6754561094166666E-5	0.0	0.0	3.350912218833333E-5	5.0263683282499994E-5
12	1.6754561094166666E-5	0.0	0.0	3.350912218833333E-5	2.1780929422416664E-4
13	1.6754561094166666E-5	0.0	0.0	3.350912218833333E-5	2.345638553183333E-4
14	1.6754561094166666E-5	0.0	0.0	3.350912218833333E-5	2.345638553183333E-4
15	1.6754561094166666E-5	0.0	0.0	3.350912218833333E-5	2.345638553183333E-4
16	1.6754561094166666E-5	0.0	0.0	1.0052736656499999E-4	2.345638553183333E-4
17	1.6754561094166666E-5	0.0	0.0	1.1728192765916665E-4	2.345638553183333E-4
18	1.6754561094166666E-5	0.0	0.0	1.507910498475E-4	2.6807297750666665E-4
19	1.6754561094166666E-5	0.0	0.0	2.0105473312999998E-4	2.6807297750666665E-4
20	1.6754561094166666E-5	0.0	0.0	2.848275386008333E-4	3.01582099695E-4
21	1.6754561094166666E-5	0.0	0.0	4.0210946625999995E-4	3.01582099695E-4
22	1.6754561094166666E-5	0.0	0.0	4.858822717308333E-4	3.686003440716666E-4
23	1.6754561094166666E-5	0.0	0.0	6.869370048608333E-4	3.686003440716666E-4
24	1.6754561094166666E-5	0.0	0.0	9.717645434616666E-4	3.686003440716666E-4
25	1.6754561094166666E-5	0.0	0.0	0.00120632839878	3.686003440716666E-4
26	1.6754561094166666E-5	0.0	0.0	0.0013403648875333332	4.0210946625999995E-4
27	1.6754561094166666E-5	0.0	0.0	0.0017927380370758332	4.0210946625999995E-4
28	1.6754561094166666E-5	0.0	0.0	0.003551966951963333	4.0210946625999995E-4
29	1.6754561094166666E-5	0.0	0.0	0.008276753180518332	4.0210946625999995E-4
30	1.6754561094166666E-5	0.0	0.0	0.0156320055008575	4.0210946625999995E-4
31	1.6754561094166666E-5	0.0	0.0	0.03546940583635083	4.5237314954249997E-4
32	1.6754561094166666E-5	0.0	0.0	0.05763569016393333	4.5237314954249997E-4
33	1.6754561094166666E-5	0.0	0.0	0.07924907397540833	4.5237314954249997E-4
34	1.6754561094166666E-5	0.0	0.0	0.10374424229507999	4.691277106366666E-4
35	1.6754561094166666E-5	0.0	0.0	0.13247831457157583	4.691277106366666E-4
36	1.6754561094166666E-5	0.0	0.0	0.17746431110941333	4.691277106366666E-4
37	1.6754561094166666E-5	0.0	0.0	0.24779995858272497	4.691277106366666E-4
38	1.6754561094166666E-5	0.0	0.0	0.3478581974370883	4.858822717308333E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8685	0.0	28.04159	1
GGTATCA	3670	0.0	26.25626	1
CGCAATA	1520	0.0	19.105997	36
CGTCGTA	1555	0.0	18.816025	10
ACCGTCG	1605	0.0	18.366768	8
ATACCGT	1685	0.0	18.147394	6
TCAACGC	13255	0.0	18.140104	4
TACCGTC	1740	0.0	18.079489	7
TATCAAC	13725	0.0	17.871237	2
CCGTCGT	1630	0.0	17.815443	9
ATCAACG	13615	0.0	17.69277	3
CAACGCA	13630	0.0	17.65716	5
AACGCAG	13710	0.0	17.554127	6
CTAGCGG	1635	0.0	17.493614	29
ATACGAA	1705	0.0	16.902744	40
TAGCGGC	1740	0.0	16.43824	30
TCTAGCG	1775	0.0	16.23779	28
CGAATGC	1810	0.0	15.800786	43
CAATACG	1815	0.0	15.636572	38
AATACGA	1865	0.0	15.452771	39
>>END_MODULE