FastQCFastQC Report
Thu 2 Feb 2017
SRR4064019_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064019_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786438
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57880.20772039428115752No Hit
TATCAACGCAGAGTACTTTTTTTTT37280.13379088284038618No Hit
GTCCTAAAGTGTGTATTTCTCATTT33050.11861021131638315No Hit
GTCCTACAGTGGACATTTCTAAATT29370.10540338597162398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC400.005274965414.2522515
GTTCACG500.001503971613.2954131
GTATCAA35950.012.5742431
AACCGCG2200.012.0908637
AACCGTG951.0407912E-611.9999547
GGTATCA13350.011.80866051
CGTAAAT1052.736142E-711.7574263
CGCATCG3650.011.71354613
ACGTTCG907.4666987E-611.6110687
AGGCCCG4450.011.315783510
GTAATAC1850.011.2929963
CGAGCCG4150.011.21863815
TGTGCGA600.00587309311.08448510
AAGACGG4250.010.9513265
GACCTAT700.001496344110.8533981
CGGACCA4300.010.8267089
TAGAACC4500.010.763924
GGACGTG17050.010.7530456
AGGACGT17000.010.727835
CGGTAAT1355.721995E-810.5519151
Produced by FastQC (version 0.11.5)