##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064019_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2786438
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.002745081713645	32.0	32.0	32.0	32.0	32.0
2	30.355918559824406	32.0	32.0	32.0	21.0	32.0
3	30.375877733507796	32.0	32.0	32.0	21.0	32.0
4	30.334445625562097	32.0	32.0	32.0	21.0	32.0
5	30.261426595531642	32.0	32.0	32.0	21.0	32.0
6	33.59359260819728	36.0	36.0	36.0	21.0	36.0
7	33.59128859138441	36.0	36.0	36.0	21.0	36.0
8	33.46547635368165	36.0	36.0	36.0	21.0	36.0
9	33.82080886063139	36.0	36.0	36.0	32.0	36.0
10	33.186243871207616	36.0	32.0	36.0	21.0	36.0
11	33.85112677906345	36.0	36.0	36.0	32.0	36.0
12	33.46003930466065	36.0	36.0	36.0	21.0	36.0
13	33.667448190126606	36.0	36.0	36.0	27.0	36.0
14	33.41792388705581	36.0	36.0	36.0	21.0	36.0
15	33.27970010457796	36.0	36.0	36.0	21.0	36.0
16	33.359240722384634	36.0	36.0	36.0	21.0	36.0
17	33.21990907387855	36.0	36.0	36.0	21.0	36.0
18	33.25117156742766	36.0	36.0	36.0	21.0	36.0
19	33.24432985768928	36.0	36.0	36.0	21.0	36.0
20	33.19516709146229	36.0	36.0	36.0	21.0	36.0
21	33.18388745775072	36.0	36.0	36.0	21.0	36.0
22	33.12679736638676	36.0	36.0	36.0	21.0	36.0
23	33.06782602017343	36.0	32.0	36.0	21.0	36.0
24	33.05120551758195	36.0	32.0	36.0	21.0	36.0
25	32.141854582804285	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	18.0
5	30.0
6	110.0
7	56.0
8	138.0
9	171.0
10	240.0
11	106.0
12	129.0
13	111.0
14	491.0
15	1202.0
16	1870.0
17	2473.0
18	2992.0
19	3861.0
20	5241.0
21	7007.0
22	9731.0
23	13455.0
24	19033.0
25	27700.0
26	39803.0
27	55592.0
28	79458.0
29	111083.0
30	155974.0
31	224525.0
32	327361.0
33	445079.0
34	701108.0
35	550283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82257561326561	18.13479798826548	12.000831461408309	26.041794937060597
2	15.577170925198356	20.095151651533634	39.41235098010293	24.915326443165085
3	18.243628075335835	24.619410760037226	29.485505147676054	27.65145601695089
4	12.20468597695341	16.139251409367645	37.28095046002736	34.37511215365158
5	13.813256804375243	37.06833123850146	34.613106923999595	14.505305033123705
6	32.537490537858794	36.61483441208024	17.658787202052473	13.188887848008491
7	28.88876604820771	31.02796472095403	21.84071017547991	18.242559055358353
8	26.75779861700959	34.54575348996502	20.0570226862984	18.639425206726983
9	27.12705036043547	14.51766130196227	19.071448728692157	39.28383960891011
10	15.169608861197649	27.18520960173731	32.93828788263969	24.706893654425357
11	36.00617012880647	21.135563661589405	24.238186217675853	18.620079991928275
12	24.67037850318786	23.433047793635776	30.306181626229673	21.590392076946692
13	29.016313028589813	20.36241439143225	25.89921314809708	24.72205943188086
14	22.671855563184643	20.583524619128195	26.094638347750394	30.649981469936765
15	23.88539640464237	29.075945762619355	22.745378687471476	24.293279145266798
16	24.144184389624492	26.36315712360919	25.647032799082993	23.845625687683324
17	22.74260426104015	26.855770926090912	26.54440352009273	23.857221292776213
18	23.34915155866325	25.68219912719024	28.0555793992187	22.913069914927807
19	24.875198963379248	25.227945950915664	26.144147172565173	23.752707913139915
20	25.068956996692936	25.417477150811823	26.021138192694583	23.492427659800658
21	25.427817454018374	24.880713899389544	25.309159836551288	24.38230881004079
22	24.8798468015294	25.713897833686854	25.66325786661361	23.742997498170137
23	23.251493132613593	25.54029868758052	26.471142826049554	24.73706535375633
24	24.231019937617507	25.52661491469544	26.488246268991734	23.75411887869532
25	23.89267414251764	25.558560852596106	26.516201488106887	24.032563516779362
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	178.0
1	178.0
2	555.0
3	932.0
4	932.0
5	932.0
6	2506.5
7	4081.0
8	4081.0
9	4081.0
10	4735.5
11	5390.0
12	5390.0
13	5390.0
14	7072.5
15	8755.0
16	8755.0
17	8755.0
18	15651.0
19	22547.0
20	22547.0
21	22547.0
22	36934.5
23	51322.0
24	51322.0
25	51322.0
26	76937.0
27	102552.0
28	102552.0
29	102552.0
30	131737.0
31	160922.0
32	160922.0
33	160922.0
34	187753.0
35	214584.0
36	214584.0
37	214584.0
38	238281.5
39	261979.0
40	261979.0
41	261979.0
42	289790.0
43	317601.0
44	317601.0
45	317601.0
46	341186.0
47	364771.0
48	364771.0
49	364771.0
50	362028.5
51	359286.0
52	359286.0
53	359286.0
54	327986.0
55	296686.0
56	296686.0
57	296686.0
58	265846.0
59	235006.0
60	235006.0
61	235006.0
62	201682.0
63	168358.0
64	168358.0
65	168358.0
66	136366.0
67	104374.0
68	104374.0
69	104374.0
70	78277.5
71	52181.0
72	52181.0
73	52181.0
74	39231.5
75	26282.0
76	26282.0
77	26282.0
78	21535.5
79	16789.0
80	16789.0
81	16789.0
82	11422.5
83	6056.0
84	6056.0
85	6056.0
86	4583.5
87	3111.0
88	3111.0
89	3111.0
90	2159.5
91	1208.0
92	1208.0
93	1208.0
94	764.0
95	320.0
96	320.0
97	320.0
98	743.5
99	1167.0
100	1167.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035206238215241104
2	0.005347328740133461
3	0.0012560839322461148
4	0.002942825212690898
5	0.007751832267576024
6	0.012022517637212815
7	0.019200140107190615
8	0.03158153886790232
9	0.04292218237046724
10	0.05175065800853994
11	0.05049457407629382
12	0.05932304971436651
13	0.059933147624314634
14	0.06366551130870308
15	0.056308448276975835
16	0.06194288191590841
17	0.058318182568569625
18	0.07525737159771723
19	0.07199155337387733
20	0.07514970726066757
21	0.07019714775638287
22	0.07450372123836957
23	0.0786308541586068
24	0.07188388903682767
25	0.07518559537301746
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2786438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.575687195892606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.0495985159436	36.45585821687802
2	13.959138266696142	13.561494679344927
3	4.540612082655046	6.616900566148259
4	2.0413350457468074	3.966370105768402
5	1.075646216605166	2.612512707562894
6	0.6702350421963458	1.9534276654473344
7	0.4803465311072271	1.6333213988487731
8	0.32775740813350784	1.2736833066903828
9	0.2575557128012352	1.125985116649316
>10	1.4028233968386916	12.436556007600506
>50	0.10390715609814838	3.5229218689871633
>100	0.07606330546733697	7.636137914385636
>500	0.010605587716019719	3.541837279089874
>1k	0.004301567045643396	3.454473904941164
>5k	7.416494906281718E-5	0.20851926165734455
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5788	0.20772039428115752	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3728	0.13379088284038618	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3305	0.11861021131638315	No Hit
GTCCTACAGTGGACATTTCTAAATT	2937	0.10540338597162398	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.1776224699778E-5
2	0.0	0.0	0.0	0.0	7.1776224699778E-5
3	0.0	0.0	0.0	0.0	7.1776224699778E-5
4	0.0	0.0	0.0	0.0	7.1776224699778E-5
5	0.0	0.0	0.0	0.0	7.1776224699778E-5
6	0.0	0.0	0.0	0.0	7.1776224699778E-5
7	0.0	0.0	0.0	0.0	7.1776224699778E-5
8	0.0	0.0	0.0	0.0	7.1776224699778E-5
9	0.0	0.0	0.0	0.0	7.1776224699778E-5
10	0.0	0.0	0.0	0.0	7.1776224699778E-5
11	0.0	0.0	0.0	0.0	7.1776224699778E-5
12	0.0	0.0	0.0	0.0	1.0766433704966699E-4
13	0.0	0.0	0.0	0.0	1.7944056174944498E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATC	40	0.0052749654	14.25225	15
GTTCACG	50	0.0015039716	13.295413	1
GTATCAA	3595	0.0	12.574243	1
AACCGCG	220	0.0	12.090863	7
AACCGTG	95	1.0407912E-6	11.999954	7
GGTATCA	1335	0.0	11.8086605	1
CGTAAAT	105	2.736142E-7	11.757426	3
CGCATCG	365	0.0	11.713546	13
ACGTTCG	90	7.4666987E-6	11.611068	7
AGGCCCG	445	0.0	11.3157835	10
GTAATAC	185	0.0	11.292996	3
CGAGCCG	415	0.0	11.218638	15
TGTGCGA	60	0.005873093	11.084485	10
AAGACGG	425	0.0	10.951326	5
GACCTAT	70	0.0014963441	10.853398	1
CGGACCA	430	0.0	10.826708	9
TAGAACC	450	0.0	10.76392	4
GGACGTG	1705	0.0	10.753045	6
AGGACGT	1700	0.0	10.72783	5
CGGTAAT	135	5.721995E-8	10.551915	1
>>END_MODULE