##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2786438
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.067374547720064	32.0	32.0	32.0	32.0	32.0
2	30.799872094767586	32.0	32.0	32.0	32.0	32.0
3	31.141085500556624	32.0	32.0	32.0	32.0	32.0
4	31.337025980840053	32.0	32.0	32.0	32.0	32.0
5	31.17514331917667	32.0	32.0	32.0	32.0	32.0
6	34.764449810116	36.0	36.0	36.0	32.0	36.0
7	34.79942564664995	36.0	36.0	36.0	32.0	36.0
8	34.70586103118031	36.0	36.0	36.0	32.0	36.0
9	34.92845776579274	36.0	36.0	36.0	32.0	36.0
10	34.63983802977134	36.0	36.0	36.0	32.0	36.0
11	34.93543549147693	36.0	36.0	36.0	36.0	36.0
12	34.787132891526745	36.0	36.0	36.0	32.0	36.0
13	34.87798544234611	36.0	36.0	36.0	32.0	36.0
14	34.79877212412406	36.0	36.0	36.0	32.0	36.0
15	34.72487060541092	36.0	36.0	36.0	32.0	36.0
16	34.768002374357515	36.0	36.0	36.0	32.0	36.0
17	34.71984447527632	36.0	36.0	36.0	32.0	36.0
18	34.73047238086762	36.0	36.0	36.0	32.0	36.0
19	34.73422843070615	36.0	36.0	36.0	32.0	36.0
20	34.73069704045093	36.0	36.0	36.0	32.0	36.0
21	34.726019742768365	36.0	36.0	36.0	32.0	36.0
22	34.70086253489222	36.0	36.0	36.0	32.0	36.0
23	34.642074576932984	36.0	36.0	36.0	32.0	36.0
24	34.61826424991333	36.0	36.0	36.0	32.0	36.0
25	34.5795348757087	36.0	36.0	36.0	32.0	36.0
26	34.50856828682354	36.0	36.0	36.0	32.0	36.0
27	34.49514290287457	36.0	36.0	36.0	32.0	36.0
28	34.46399919897733	36.0	36.0	36.0	32.0	36.0
29	34.43306364613173	36.0	36.0	36.0	32.0	36.0
30	34.410489305701404	36.0	36.0	36.0	32.0	36.0
31	34.41143675186744	36.0	36.0	36.0	32.0	36.0
32	34.36085855848937	36.0	36.0	36.0	32.0	36.0
33	34.334677821649	36.0	36.0	36.0	32.0	36.0
34	34.32688866574458	36.0	36.0	36.0	32.0	36.0
35	34.29749091851317	36.0	36.0	36.0	32.0	36.0
36	34.2485330734077	36.0	36.0	36.0	32.0	36.0
37	34.231377837942205	36.0	36.0	36.0	32.0	36.0
38	34.18739480297067	36.0	36.0	36.0	32.0	36.0
39	34.17606995023754	36.0	36.0	36.0	32.0	36.0
40	34.16757523404433	36.0	36.0	36.0	32.0	36.0
41	34.14460217668579	36.0	36.0	36.0	32.0	36.0
42	34.08200146567051	36.0	36.0	36.0	32.0	36.0
43	34.078681815278145	36.0	36.0	36.0	32.0	36.0
44	33.99747455353394	36.0	36.0	36.0	32.0	36.0
45	33.958912059051734	36.0	36.0	36.0	32.0	36.0
46	33.93493269902291	36.0	36.0	36.0	32.0	36.0
47	33.919245287352524	36.0	36.0	36.0	32.0	36.0
48	33.86711636863982	36.0	36.0	36.0	32.0	36.0
49	33.84525225395289	36.0	36.0	36.0	32.0	36.0
50	33.12341132298655	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	8.0
20	18.0
21	75.0
22	235.0
23	739.0
24	1974.0
25	4794.0
26	9987.0
27	19460.0
28	34510.0
29	56133.0
30	86772.0
31	134027.0
32	205802.0
33	347852.0
34	663612.0
35	1220438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.493780401274385	19.374160392754046	12.800504473179764	26.331554732791794
2	15.004674448442001	21.2947414698045	38.31748566711826	25.383098414635242
3	17.732354494923975	25.42477261243052	29.739394322201296	27.10347857044421
4	12.10757246348205	17.175548137083975	37.44379024403199	33.27308915540198
5	13.956240906849535	37.34516971129449	33.75754278401314	14.941046597842838
6	32.77589464674396	37.25997556016918	17.12460545464007	12.839524338446799
7	28.89615982566978	31.3271146367618	21.631216363842558	18.145509173725863
8	26.865087254767555	34.80543977651755	19.64709065839613	18.682382310318765
9	27.156840297242262	14.302827935834372	18.77036219312708	39.76996957379628
10	15.026020909319616	27.524024615159348	32.779239832716826	24.670714642804207
11	36.37130989456791	21.10917235553061	23.99927793117952	18.52023981872197
12	24.667137041205383	23.514910825026412	30.063931221002342	21.75402091276586
13	29.134145938814854	20.379819611344704	25.772566167150202	24.71346828269024
14	22.749763227414014	20.465046839521122	25.932510751215982	30.85267918184888
15	24.066718706539977	29.128296232492616	22.482732631692283	24.32225242927512
16	24.361178730708676	26.38022036153187	25.35535728448788	23.90324362327157
17	22.972554924961546	26.85112677906345	26.314563611320256	23.861754684654745
18	23.662464156644884	25.67076868968537	27.760898353807562	22.90586879986219
19	25.088545040526693	25.21960856793717	26.00613328500396	23.685713106532184
20	25.320616919529503	25.428748306521797	25.73634675255928	23.514288021389415
21	25.63581174244681	24.796819451931103	25.223457331546584	24.343911474075504
22	25.07965377970553	25.68174950492278	25.545697475698333	23.69289923967336
23	23.517967633819225	25.45047833659378	26.304582894150823	24.72697113543617
24	24.451278355717402	25.554553477189994	26.158228931561556	23.835939235531043
25	24.19760902184941	25.44370118910088	26.319708899432992	24.038980889616713
26	23.45158834779744	26.27559690674771	26.712516818760783	23.56029792669407
27	24.074203011733832	25.973243388548696	25.82634617496596	24.126207424751517
28	23.394607196601424	25.959259143615842	26.217662362458604	24.42847129732413
29	23.94813342042363	25.478506162117153	25.900487370710383	24.672873046748826
30	23.602777827623953	25.746442478511316	26.373607048647873	24.27717264521686
31	24.578080613583467	25.181937762336897	25.838374448856978	24.401607175222654
32	23.96740841017484	26.010788974731575	25.608938964521133	24.41286365057245
33	23.461078248336346	25.840692465440252	26.447260091575526	24.25096919464788
34	24.181814462425965	25.741257739883917	26.50156349872002	23.575364298970097
35	24.483485311344374	25.751480062934974	26.193560936674555	23.571473689046098
36	23.654534700428677	26.08802231531436	26.09642070626879	24.161022277988174
37	24.37115963879744	25.819321917958167	25.53949437240499	24.270024070839398
38	24.045039878086687	26.069235909874305	25.817498982500442	24.068225229538566
39	24.796965491401114	25.30429605193032	25.509151940406603	24.38958651626196
40	25.12445336879146	25.55074047556242	26.071451001925432	23.25335515372068
41	24.2261078033427	25.448522629801495	26.52117270857783	23.804196858277972
42	24.750035529766766	26.425246088495673	25.82285504285823	23.00186333887933
43	23.831183373002087	25.862795398233136	26.30587480368991	24.000146425074863
44	23.617864120596668	26.15392124352034	25.624064208738695	24.6041504271443
45	23.80422428405265	26.390631970206858	25.687936425791868	24.117207319948623
46	23.978894759829558	25.573379720264413	25.75852788724731	24.689197632658715
47	24.693035045666566	25.527734242800637	25.828948034250732	23.950282677282065
48	24.561905951360686	26.14564087987746	25.097832749782157	24.1946204189797
49	24.36705748933223	25.93881777040873	25.37088676191399	24.32323797834505
50	24.2599063165284	26.589216782634534	24.983168269927837	24.167708630909228
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	26.5
2	39.0
3	113.5
4	188.0
5	261.5
6	335.0
7	338.5
8	342.0
9	479.0
10	616.0
11	935.5
12	1255.0
13	2296.0
14	3337.0
15	4557.0
16	5777.0
17	6165.0
18	6553.0
19	6689.0
20	6825.0
21	8211.5
22	9598.0
23	11856.5
24	14115.0
25	17917.5
26	21720.0
27	28551.0
28	35382.0
29	42770.0
30	50158.0
31	58500.0
32	66842.0
33	78803.5
34	90765.0
35	105133.5
36	119502.0
37	129327.0
38	139152.0
39	142855.0
40	146558.0
41	152258.5
42	157959.0
43	158404.0
44	158849.0
45	169989.5
46	181130.0
47	189365.0
48	197600.0
49	201173.0
50	204746.0
51	198403.5
52	192061.0
53	187653.5
54	183246.0
55	180366.5
56	177487.0
57	170011.0
58	162535.0
59	146608.5
60	130682.0
61	114722.5
62	98763.0
63	84512.5
64	70262.0
65	59166.0
66	48070.0
67	40626.0
68	33182.0
69	30070.5
70	26959.0
71	20904.0
72	14849.0
73	12658.5
74	10468.0
75	8235.5
76	6003.0
77	5270.5
78	4538.0
79	3760.5
80	2983.0
81	2473.5
82	1964.0
83	1699.0
84	1434.0
85	1049.0
86	664.0
87	491.5
88	319.0
89	264.5
90	210.0
91	177.0
92	144.0
93	108.0
94	72.0
95	80.5
96	89.0
97	65.0
98	41.0
99	48.5
100	56.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005921538537731684
2	4.6654546054855704E-4
3	2.15328674099334E-4
4	0.0
5	0.0
6	1.07664337049667E-4
7	7.177622469977799E-5
8	0.0
9	8.61314696397336E-4
10	8.97202808747225E-4
11	0.0
12	2.15328674099334E-4
13	3.5888112349888996E-4
14	3.22993011149001E-4
15	0.004055356695537457
16	6.100979099481129E-4
17	0.0
18	2.8710489879911195E-4
19	1.07664337049667E-4
20	4.6654546054855704E-4
21	0.0
22	1.4355244939955598E-4
23	3.94769235848779E-4
24	2.51216786449223E-4
25	0.0016867412804447831
26	0.004127132920237234
27	0.0048807832795849035
28	0.003409370673239455
29	0.0020815105162935617
30	0.003158153886790232
31	0.006567524560029687
32	0.0026916084262416746
33	0.0037323636843884563
34	0.0021173986286434506
35	0.0025121678644922296
36	0.00681874134647891
37	0.0028351608756412307
38	0.007034070020578244
39	0.0031940419991401212
40	0.001902069954544117
41	0.0024403916397924517
42	0.0015073007186953379
43	0.0010766433704966698
44	9.330909210971141E-4
45	8.97202808747225E-4
46	7.536503593476689E-4
47	0.001614965055745005
48	0.00179440561749445
49	2.8710489879911195E-4
50	0.0012201958198962259
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2786438.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.189073416837054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5792469048097	42.03947506147661
2	12.79079832767672	13.862430192768608
3	4.054667135635723	6.591559652814018
4	1.823313181518215	3.952146074207092
5	0.9675655914640323	2.6215741435724906
6	0.5808388823900773	1.8885072504713687
7	0.3870150748516948	1.4680391813192823
8	0.28382285832584725	1.2304078165756702
9	0.21513896582068184	1.0492363092311394
>10	1.1717494719127262	11.464995694919283
>50	0.0788788886636867	2.9421172034210015
>100	0.05776991212475222	6.381071271603715
>500	0.006729531053841812	2.5596738622114783
>1k	0.0024652737523671434	1.9487662854082088
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.5888112349889E-5
2	0.0	0.0	0.0	0.0	3.5888112349889E-5
3	0.0	0.0	0.0	0.0	3.5888112349889E-5
4	0.0	0.0	0.0	0.0	3.5888112349889E-5
5	0.0	0.0	0.0	0.0	3.5888112349889E-5
6	0.0	0.0	0.0	0.0	3.5888112349889E-5
7	0.0	0.0	0.0	3.5888112349889E-5	3.5888112349889E-5
8	0.0	0.0	0.0	3.5888112349889E-5	3.5888112349889E-5
9	0.0	0.0	0.0	3.5888112349889E-5	3.5888112349889E-5
10	0.0	0.0	0.0	3.5888112349889E-5	3.5888112349889E-5
11	0.0	0.0	0.0	3.5888112349889E-5	3.5888112349889E-5
12	0.0	0.0	0.0	3.5888112349889E-5	1.43552449399556E-4
13	0.0	0.0	0.0	3.5888112349889E-5	1.7944056174944498E-4
14	0.0	0.0	0.0	3.5888112349889E-5	1.7944056174944498E-4
15	0.0	0.0	0.0	3.5888112349889E-5	1.7944056174944498E-4
16	0.0	0.0	0.0	3.5888112349889E-5	2.51216786449223E-4
17	0.0	0.0	0.0	3.5888112349889E-5	2.51216786449223E-4
18	0.0	0.0	0.0	3.5888112349889E-5	2.51216786449223E-4
19	0.0	0.0	0.0	3.5888112349889E-5	2.51216786449223E-4
20	0.0	0.0	0.0	3.5888112349889E-5	2.87104898799112E-4
21	0.0	0.0	0.0	3.5888112349889E-5	3.22993011149001E-4
22	0.0	0.0	0.0	3.5888112349889E-5	3.22993011149001E-4
23	0.0	0.0	0.0	3.5888112349889E-5	3.5888112349888996E-4
24	0.0	0.0	0.0	3.5888112349889E-5	3.5888112349888996E-4
25	0.0	0.0	0.0	3.5888112349889E-5	3.5888112349888996E-4
26	0.0	0.0	0.0	3.5888112349889E-5	3.5888112349888996E-4
27	0.0	0.0	0.0	3.5888112349889E-5	3.5888112349888996E-4
28	0.0	0.0	0.0	3.5888112349889E-5	3.5888112349888996E-4
29	0.0	0.0	0.0	1.7944056174944498E-4	3.5888112349888996E-4
30	0.0	0.0	0.0	3.22993011149001E-4	3.5888112349888996E-4
31	0.0	0.0	0.0	6.81874134647891E-4	4.3065734819866796E-4
32	0.0	0.0	0.0	0.001184307707546337	4.66545460548557E-4
33	0.0	0.0	0.0	0.00215328674099334	4.66545460548557E-4
34	0.0	0.0	0.0	0.002978713325040787	5.02433572898446E-4
35	0.0	0.0	0.0	0.0050602238413343485	5.02433572898446E-4
36	0.0	0.0	0.0	0.007644167930526357	5.02433572898446E-4
37	0.0	0.0	0.0	0.012381398760711704	5.02433572898446E-4
38	0.0	0.0	0.0	0.02113809817408462	5.02433572898446E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3665	0.0	25.390444	1
ATACCGT	630	0.0	19.903187	6
CGTCGTA	620	0.0	19.87011	10
ACCGTCG	620	0.0	19.514584	8
CCGTCGT	635	0.0	19.400736	9
ACCGTAT	115	2.0254447E-7	19.128922	8
GTCGTAG	645	0.0	19.099949	11
TACCGTC	660	0.0	18.998497	7
AACGCAG	5080	0.0	18.10093	6
TAGGACC	1935	0.0	17.621346	4
TGTAGGA	2550	0.0	17.512653	2
GTATAGG	290	0.0	17.447523	1
GTAGGAC	2445	0.0	17.364677	3
TTAGGAC	1540	0.0	17.284346	3
TCAACGC	5405	0.0	16.93113	4
TAGGACG	2205	0.0	16.860344	4
GGTATCA	1730	0.0	16.7854	1
CAACGCA	5490	0.0	16.668991	5
CTAGCGG	690	0.0	16.580183	29
ATCAACG	5590	0.0	16.41015	3
>>END_MODULE