FastQCFastQC Report
Thu 2 Feb 2017
SRR4064018_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064018_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195913
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44400.37126446489000453No Hit
TATCAACGCAGAGTACTTTTTTTTT30940.25871447170488154No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27530.2302006918563474No Hit
GTCCTAAAGTGTGTATTTCTCATTT25300.21155385048912423No Hit
GTCCTACAGTGGACATTTCTAAATT23820.19917836832612407No Hit
CTTTAGGACGTGAAATATGGCGAGG21250.17768851078631975No Hit
CTGTAGGACGTGGAATATGGCAAGA21010.17568167584096836No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17960.1501781484104613No Hit
GGTATCAACGCAGAGTACTTTTTTT15760.13178216141140703No Hit
TTGTAGAACAGTGTATATCAATGAG15340.1282702002570421No Hit
GTACATGGGGTGGTATCAACGCAAA15290.12785210964342725No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15090.12617974718896777No Hit
GTCCTACAGTGTGCATTTCTCATTT15040.1257616565753529No Hit
GATATACACTGTTCTACAAATCCCG13800.11539300935770411No Hit
ATTTAGAAATGTCCACTGTAGGACG12280.10268305470381206No Hit
GTGTATATCAATGAGTTACAATGAA12060.10084345600390665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCG350.002168048916.28768710
GCGTTAT953.074092E-1014.99303151
CGAACGA653.364852E-614.61776716
TATGTCG759.649557E-713.93560416
TCTATAC759.704363E-713.9286093
CGGCCAC604.0851752E-412.66873114
AAGACGG1600.012.467135
TCCAACG2300.012.39384318
CGTTATT1001.4508623E-712.3437472
TGTCGAG853.9321403E-612.29663718
GGTTATG853.9616843E-612.2884071
ATAATTC550.003075533512.086813
ATGTCGA951.0370495E-612.00195617
AACCCGG802.8627379E-511.87594111
TTGGACT1052.7251735E-711.7593934
GACGTGG4700.011.7233537
CCCGGTG907.4428935E-611.61300413
CCGATAA907.4463624E-611.6125189
CGGTCCA1501.7644197E-1011.401381510
GGGTCGC752.074747E-411.3989966
Produced by FastQC (version 0.11.5)