##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064018_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1195913
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.179729629161987	32.0	32.0	32.0	32.0	32.0
2	30.747969124844367	32.0	32.0	32.0	32.0	32.0
3	30.75846905251469	32.0	32.0	32.0	32.0	32.0
4	30.783275204801686	32.0	32.0	32.0	32.0	32.0
5	30.711291707674388	32.0	32.0	32.0	32.0	32.0
6	34.367765046454046	36.0	36.0	36.0	32.0	36.0
7	34.282931116226685	36.0	36.0	36.0	32.0	36.0
8	34.22768796726852	36.0	36.0	36.0	32.0	36.0
9	34.37069168074935	36.0	36.0	36.0	32.0	36.0
10	34.05704595568407	36.0	36.0	36.0	32.0	36.0
11	34.38834764736231	36.0	36.0	36.0	32.0	36.0
12	34.15995143459432	36.0	36.0	36.0	32.0	36.0
13	34.24750462617264	36.0	36.0	36.0	32.0	36.0
14	34.15537334237524	36.0	36.0	36.0	32.0	36.0
15	34.07934858137674	36.0	36.0	36.0	32.0	36.0
16	34.09094056172982	36.0	36.0	36.0	32.0	36.0
17	34.03109339893454	36.0	36.0	36.0	32.0	36.0
18	34.02526939668688	36.0	36.0	36.0	32.0	36.0
19	34.02967774411684	36.0	36.0	36.0	32.0	36.0
20	33.99112560863541	36.0	36.0	36.0	32.0	36.0
21	33.97509768687187	36.0	36.0	36.0	32.0	36.0
22	33.9385833250412	36.0	36.0	36.0	32.0	36.0
23	33.8985979749363	36.0	36.0	36.0	32.0	36.0
24	33.88425244980195	36.0	36.0	36.0	32.0	36.0
25	33.48067125284197	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	14.0
5	13.0
6	48.0
7	17.0
8	72.0
9	84.0
10	113.0
11	39.0
12	57.0
13	60.0
14	135.0
15	235.0
16	393.0
17	492.0
18	614.0
19	842.0
20	1343.0
21	1987.0
22	3171.0
23	4853.0
24	7114.0
25	10358.0
26	14720.0
27	19510.0
28	26118.0
29	34867.0
30	45629.0
31	60992.0
32	85005.0
33	119319.0
34	249639.0
35	508057.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.8480174129374	17.885234889784705	11.73901004907845	25.527737648199437
2	15.085964677548835	20.44094527696013	40.61672130050843	23.856368744982607
3	18.92663681989755	25.183754973300132	29.33891692017467	26.550691286627654
4	11.921924232186939	16.198079380263977	37.63312550591037	34.24687088163871
5	13.24607207249397	38.09362132002448	34.606069931660585	14.054236675820961
6	31.78185984469933	37.16294025900171	18.07408770264563	12.98111219365333
7	28.149764518829713	31.345634168229992	21.952733145595193	18.551868167345106
8	26.21629986532493	35.752883803023074	20.22769287393243	17.803123457719558
9	27.461639690423663	14.146276575719623	19.36500207895686	39.02708165489985
10	15.352448523690398	27.939776273625554	33.17232954878222	23.535445653901824
11	36.06704684644887	21.097969920282726	24.60777226695582	18.22721096631259
12	24.60048981258435	23.83924709095336	31.089209647985903	20.471053448476383
13	29.728716612530476	20.791643447512996	25.917763196428183	23.56187674352834
14	22.114375699724043	21.368421757254957	26.543994966170594	29.97320757685041
15	23.438196813348362	30.537336435813057	23.118828822270917	22.905637928567664
16	23.15605302594817	26.409971274682814	27.381762226668105	23.05221347270091
17	21.933548646206365	27.21627229826969	27.422018809197095	23.42816024632685
18	22.417061016481103	26.015204957301435	29.827741306521734	21.739992719695735
19	24.78163801197288	24.917366367818826	26.691374471353953	23.609621148854345
20	24.751188512620704	25.89497238868233	26.93816889138823	22.415670207308736
21	25.194368969248238	24.953117978679874	25.701807750658773	24.150705301413115
22	24.482300388787262	26.577366656680596	26.355357916917015	22.584975037615127
23	22.942271288623473	26.39016046074511	27.09471820200948	23.572850048621934
24	23.99224423464119	25.94733644523516	27.350704864173686	22.709714455949964
25	23.469519257161604	26.184534688330558	27.55417645759274	22.791769596915103
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	360.0
1	360.0
2	582.0
3	804.0
4	804.0
5	804.0
6	2202.5
7	3601.0
8	3601.0
9	3601.0
10	3601.0
11	3601.0
12	3601.0
13	3601.0
14	3836.0
15	4071.0
16	4071.0
17	4071.0
18	7277.5
19	10484.0
20	10484.0
21	10484.0
22	17695.5
23	24907.0
24	24907.0
25	24907.0
26	38524.5
27	52142.0
28	52142.0
29	52142.0
30	68618.0
31	85094.0
32	85094.0
33	85094.0
34	93236.0
35	101378.0
36	101378.0
37	101378.0
38	107793.0
39	114208.0
40	114208.0
41	114208.0
42	123997.5
43	133787.0
44	133787.0
45	133787.0
46	143134.5
47	152482.0
48	152482.0
49	152482.0
50	150244.0
51	148006.0
52	148006.0
53	148006.0
54	131366.0
55	114726.0
56	114726.0
57	114726.0
58	103593.0
59	92460.0
60	92460.0
61	92460.0
62	79924.0
63	67388.0
64	67388.0
65	67388.0
66	54826.0
67	42264.0
68	42264.0
69	42264.0
70	31769.5
71	21275.0
72	21275.0
73	21275.0
74	16213.5
75	11152.0
76	11152.0
77	11152.0
78	8855.5
79	6559.0
80	6559.0
81	6559.0
82	4496.5
83	2434.0
84	2434.0
85	2434.0
86	1860.0
87	1286.0
88	1286.0
89	1286.0
90	935.0
91	584.0
92	584.0
93	584.0
94	415.0
95	246.0
96	246.0
97	246.0
98	430.0
99	614.0
100	614.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04013669890702752
2	0.006104122958777102
3	0.002257689313520298
4	0.004097288013425726
5	0.009783320358587956
6	0.014047844617459632
7	0.023078601871540823
8	0.03704282836627748
9	0.05033810987923035
10	0.05878354027425072
11	0.05761288655612908
12	0.06371700951490619
13	0.06513851760119675
14	0.06681088005565623
15	0.06187741081500076
16	0.0668944981783792
17	0.06296444641039942
18	0.07592525543246038
19	0.07350032987349414
20	0.07793209037781176
21	0.0745873654688928
22	0.0765105822915212
23	0.08186214214579154
24	0.07784847225508879
25	0.07768123600964284
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1195913.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.888403506630965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.60422184254178	40.514749951198226
2	13.876960026382939	14.678605226414584
3	4.285268251033159	6.7992298918438925
4	1.7677522220332593	3.7397437127455406
5	0.9310873494276058	2.462186171822336
6	0.5222453787169381	1.6572434591432834
7	0.35763252790332767	1.3240229409993356
8	0.2640268775669641	1.1171168029885958
9	0.18398700395301926	0.875770101453905
>10	1.0298439415028848	9.749454193592694
>50	0.08787804338555033	3.2374818311762636
>100	0.0795844538547025	8.49373328652648
>500	0.006499922493493767	2.3311833823384283
>1k	0.0030121592043019898	3.0194790477564104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4440	0.37126446489000453	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3094	0.25871447170488154	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2753	0.2302006918563474	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2530	0.21155385048912423	No Hit
GTCCTACAGTGGACATTTCTAAATT	2382	0.19917836832612407	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2125	0.17768851078631975	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2101	0.17568167584096836	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1796	0.1501781484104613	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1576	0.13178216141140703	No Hit
TTGTAGAACAGTGTATATCAATGAG	1534	0.1282702002570421	No Hit
GTACATGGGGTGGTATCAACGCAAA	1529	0.12785210964342725	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1509	0.12617974718896777	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1504	0.1257616565753529	No Hit
GATATACACTGTTCTACAAATCCCG	1380	0.11539300935770411	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1228	0.10268305470381206	No Hit
GTGTATATCAATGAGTTACAATGAA	1206	0.10084345600390665	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.6723624544594799E-4	0.0
9	0.0	0.0	0.0	3.3447249089189597E-4	0.0
10	0.0	0.0	0.0	3.3447249089189597E-4	0.0
11	0.0	0.0	0.0	3.3447249089189597E-4	0.0
12	0.0	0.0	0.0	3.3447249089189597E-4	8.361812272297399E-5
13	0.0	0.0	0.0	3.3447249089189597E-4	8.361812272297399E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCCG	35	0.0021680489	16.287687	10
GCGTTAT	95	3.074092E-10	14.9930315	1
CGAACGA	65	3.364852E-6	14.617767	16
TATGTCG	75	9.649557E-7	13.935604	16
TCTATAC	75	9.704363E-7	13.928609	3
CGGCCAC	60	4.0851752E-4	12.668731	14
AAGACGG	160	0.0	12.46713	5
TCCAACG	230	0.0	12.393843	18
CGTTATT	100	1.4508623E-7	12.343747	2
TGTCGAG	85	3.9321403E-6	12.296637	18
GGTTATG	85	3.9616843E-6	12.288407	1
ATAATTC	55	0.0030755335	12.08681	3
ATGTCGA	95	1.0370495E-6	12.001956	17
AACCCGG	80	2.8627379E-5	11.875941	11
TTGGACT	105	2.7251735E-7	11.759393	4
GACGTGG	470	0.0	11.723353	7
CCCGGTG	90	7.4428935E-6	11.613004	13
CCGATAA	90	7.4463624E-6	11.612518	9
CGGTCCA	150	1.7644197E-10	11.4013815	10
GGGTCGC	75	2.074747E-4	11.398996	6
>>END_MODULE