FastQCFastQC Report
Thu 2 Feb 2017
SRR4064018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195913
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12870.10761652394446752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT904.0857958E-721.997723
TAGGACC9200.020.8021934
TTAGGAC10200.020.703743
GTCCTAC14800.020.5122471
GTGTTAT1101.266435E-719.9987661
TCCTACA16600.019.2148762
TGTAGGA18450.018.8381562
TAGGACA5850.018.8014724
GTCCTAA7350.018.5566791
ATCGTAA1203.1639502E-718.3322011
GCACTAC600.007416486318.3314346
TGCGTAC600.00741827518.3306716
GTAGGAC18150.018.1799353
CCTACAG17200.018.160913
TTTCGCC851.4314934E-418.11652843
GTATCAA31650.018.0715521
CTGTAGG18850.017.9723641
TAGGACG17750.017.969974
TTTAGGA11650.017.7492352
GACGTGA10300.017.727067
Produced by FastQC (version 0.11.5)