##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064018_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1195913
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24713587025143	32.0	32.0	32.0	32.0	32.0
2	31.403842085502877	32.0	32.0	32.0	32.0	32.0
3	31.484496781956548	32.0	32.0	32.0	32.0	32.0
4	31.56820521225206	32.0	32.0	32.0	32.0	32.0
5	31.52777083282814	32.0	32.0	32.0	32.0	32.0
6	35.11035501746365	36.0	36.0	36.0	36.0	36.0
7	35.13224624199252	36.0	36.0	36.0	36.0	36.0
8	35.07876910778627	36.0	36.0	36.0	36.0	36.0
9	35.183859528243275	36.0	36.0	36.0	36.0	36.0
10	35.040688578517	36.0	36.0	36.0	36.0	36.0
11	35.17719181913735	36.0	36.0	36.0	36.0	36.0
12	35.09068552645552	36.0	36.0	36.0	36.0	36.0
13	35.11922606410332	36.0	36.0	36.0	36.0	36.0
14	35.07870054092564	36.0	36.0	36.0	36.0	36.0
15	35.056246566430836	36.0	36.0	36.0	36.0	36.0
16	35.060679999297605	36.0	36.0	36.0	36.0	36.0
17	35.03441972785646	36.0	36.0	36.0	36.0	36.0
18	35.02621595383611	36.0	36.0	36.0	36.0	36.0
19	35.01902479528193	36.0	36.0	36.0	36.0	36.0
20	35.0048824621858	36.0	36.0	36.0	36.0	36.0
21	34.99650559865141	36.0	36.0	36.0	36.0	36.0
22	34.98173529345362	36.0	36.0	36.0	36.0	36.0
23	34.92974572565061	36.0	36.0	36.0	36.0	36.0
24	34.90715210889086	36.0	36.0	36.0	36.0	36.0
25	34.87669253532657	36.0	36.0	36.0	32.0	36.0
26	34.82823666938983	36.0	36.0	36.0	32.0	36.0
27	34.80846600045321	36.0	36.0	36.0	32.0	36.0
28	34.76788110840839	36.0	36.0	36.0	32.0	36.0
29	34.74271539819368	36.0	36.0	36.0	32.0	36.0
30	34.71608637083132	36.0	36.0	36.0	32.0	36.0
31	34.698259823248016	36.0	36.0	36.0	32.0	36.0
32	34.6511560623557	36.0	36.0	36.0	32.0	36.0
33	34.63970372426757	36.0	36.0	36.0	32.0	36.0
34	34.621566953448955	36.0	36.0	36.0	32.0	36.0
35	34.576487587307774	36.0	36.0	36.0	32.0	36.0
36	34.53487084762855	36.0	36.0	36.0	32.0	36.0
37	34.48883656252587	36.0	36.0	36.0	32.0	36.0
38	34.43899681665807	36.0	36.0	36.0	32.0	36.0
39	34.385973728858204	36.0	36.0	36.0	32.0	36.0
40	34.39747289309506	36.0	36.0	36.0	32.0	36.0
41	34.374400144492114	36.0	36.0	36.0	32.0	36.0
42	34.309277514334234	36.0	36.0	36.0	32.0	36.0
43	34.30942969931759	36.0	36.0	36.0	32.0	36.0
44	34.21889217693929	36.0	36.0	36.0	32.0	36.0
45	34.17429027027886	36.0	36.0	36.0	32.0	36.0
46	34.1021052534758	36.0	36.0	36.0	32.0	36.0
47	34.06433661980429	36.0	36.0	36.0	32.0	36.0
48	33.9847104262601	36.0	36.0	36.0	32.0	36.0
49	33.95145884357809	36.0	36.0	36.0	32.0	36.0
50	33.40609643009149	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	3.0
20	12.0
21	41.0
22	144.0
23	382.0
24	1009.0
25	2411.0
26	4806.0
27	9107.0
28	15140.0
29	22839.0
30	32293.0
31	44785.0
32	63465.0
33	100277.0
34	203262.0
35	695935.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.82763511321178	18.85062200412774	12.260957408020658	26.060785474639825
2	14.411643705034166	21.1698558752011	40.07707950778823	24.341420911976506
3	18.23240731527232	25.682472495816988	29.75510678488079	26.330013404029906
4	11.592816534313116	16.90056049227661	37.643122869305714	33.86350010410456
5	12.735458181322553	38.50154651717976	34.49866336430827	14.26433193718941
6	31.944323662610625	37.48645385279185	17.65062981222699	12.918592672370544
7	28.00041140185181	31.422823270293527	22.03968355504716	18.53708177280751
8	26.07056372040752	36.06929272387935	20.085675200181953	17.774468355531177
9	27.176268918805917	13.960197340914792	19.358223931766872	39.505309808512415
10	15.246367165092675	28.201819887649282	33.061431660559684	23.490381286698366
11	36.07754075756347	20.949684467013903	24.64351503830128	18.329259737121344
12	24.48294604109005	23.84602520256541	31.214974370981093	20.456054385363448
13	29.836257759820185	20.666458177244994	26.03294244354062	23.4643416193942
14	22.070036984357532	21.28695195545488	26.665362221770682	29.977648838416904
15	23.438470794731504	30.58477434288041	23.140526966717758	22.836227895670326
16	23.221011051054266	26.46408072888794	27.37828454457883	22.936623675478966
17	21.966397221202545	27.33601858998104	27.310264208182367	23.387319980634043
18	22.482749509159568	25.973904347157138	29.79250912277533	21.750837020907962
19	24.784014208403292	24.896229822930117	26.801721196877367	23.518034771789228
20	24.827390135504075	25.84185198657084	27.055660775730512	22.27509710219457
21	25.203421988054313	24.837927173632195	25.92989623827151	24.028754600041978
22	24.506463284413783	26.374163920498965	26.59182262195535	22.527550173131903
23	22.889204783008612	26.27886947069153	27.222092148172923	23.609833598126933
24	23.99404634983548	25.895869655198368	27.487049556611936	22.623034438354217
25	23.486278884248936	26.066775148968052	27.63647948731242	22.81046647947059
26	22.092077150466825	27.245504225045885	28.275821699300497	22.38659692518679
27	22.882215552788548	26.59609963565269	27.43015979602571	23.091525015533055
28	22.24858157066224	27.053889862149994	27.11727494324192	23.580253623945847
29	23.041789866609584	25.74588924715358	26.698235955206506	24.51408493103033
30	22.40197078607338	26.60728441600578	27.333953245675946	23.656791552244897
31	23.994661386032632	25.711982445459835	26.762813008021304	23.53054316048623
32	22.990059948037786	26.97778770082676	26.722993906523207	23.309158444612244
33	22.502356050932512	26.650678132920575	27.838027456460097	23.008938359686816
34	23.2605725314036	26.6470270478191	27.71393833170831	22.378462089068986
35	23.162318690305565	26.923057626086855	27.51809961183461	22.396524071772966
36	22.29566267417833	27.008209367727325	27.518399369809565	23.17772858828478
37	23.000383821946755	26.809586684806924	26.449847683730717	23.740181809515608
38	22.99109140156764	27.331325769707753	26.582879736011794	23.094703092712813
39	24.103213354818696	25.8764246107172	26.62509522491477	23.395266809549334
40	24.8103274835121	26.25688093340703	26.924842605050518	22.00794897803035
41	23.45053710031057	25.81811005580893	28.152652554717665	22.578700289162832
42	23.944319153899993	27.513301842063676	26.873358436039524	21.669020567996807
43	22.408497707582328	26.9844694242574	27.683023399133205	22.92400946902707
44	22.203463670427016	27.415191416666456	26.73486640594231	23.646478506964215
45	22.469706889963113	27.543881214049016	26.720734777769696	23.26567711821818
46	22.700336064045327	26.108555627003195	26.67666190318111	24.514446405770368
47	23.714260867238405	26.456825388330678	26.732102002863144	23.096811741567773
48	23.09575693922979	26.632393497017265	25.937759745526733	24.33408981822621
49	23.43794291696595	26.86471439668804	26.30948727618452	23.387855410161492
50	22.870615111502076	27.8589209605844	25.650202316754704	23.62026161115882
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	194.0
1	119.0
2	44.0
3	172.0
4	300.0
5	328.5
6	357.0
7	343.0
8	329.0
9	470.5
10	612.0
11	887.0
12	1162.0
13	2071.5
14	2981.0
15	3954.5
16	4928.0
17	5225.5
18	5523.0
19	5712.0
20	5901.0
21	6460.5
22	7020.0
23	8006.5
24	8993.0
25	10353.5
26	11714.0
27	15890.5
28	20067.0
29	23807.0
30	27547.0
31	32053.5
32	36560.0
33	44126.5
34	51693.0
35	59230.5
36	66768.0
37	68386.5
38	70005.0
39	68760.5
40	67516.0
41	66679.5
42	65843.0
43	64623.5
44	63404.0
45	66997.0
46	70590.0
47	73255.0
48	75920.0
49	77208.0
50	78496.0
51	75922.5
52	73349.0
53	71837.5
54	70326.0
55	69021.0
56	67716.0
57	64885.5
58	62055.0
59	56443.5
60	50832.0
61	44739.0
62	38646.0
63	33321.0
64	27996.0
65	23636.0
66	19276.0
67	16418.0
68	13560.0
69	12079.5
70	10599.0
71	8278.5
72	5958.0
73	5088.5
74	4219.0
75	3335.0
76	2451.0
77	2107.0
78	1763.0
79	1436.0
80	1109.0
81	903.5
82	698.0
83	589.0
84	480.0
85	331.0
86	182.0
87	135.0
88	88.0
89	68.0
90	48.0
91	39.0
92	30.0
93	23.5
94	17.0
95	19.5
96	22.0
97	18.0
98	14.0
99	13.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008445430395020374
2	4.1809061361486994E-4
3	3.3447249089189597E-4
4	0.0
5	0.0
6	8.361812272297399E-5
7	1.6723624544594799E-4
8	8.361812272297399E-5
9	0.0010870355953986619
10	0.00125427184084461
11	0.0
12	2.50854368168922E-4
13	7.525631045067659E-4
14	1.6723624544594799E-4
15	0.0041809061361487
16	7.525631045067659E-4
17	0.0
18	4.1809061361486994E-4
19	8.361812272297399E-5
20	6.689449817837919E-4
21	0.0
22	3.3447249089189597E-4
23	0.0010870355953986619
24	6.689449817837919E-4
25	0.001588744331736506
26	0.005686032345162232
27	0.006354977326946024
28	0.004013669890702752
29	0.00209045306807435
30	0.00376281552253383
31	0.008110957904128479
32	0.003679197399810856
33	0.004682614872486544
34	0.002592161804412194
35	0.002592161804412194
36	0.007023922308729816
37	0.003846433645256804
38	0.008529048517743347
39	0.00501708736337844
40	0.0030102524180270636
41	0.003930051767979778
42	0.0021740711907973238
43	0.001170653718121636
44	0.001170653718121636
45	0.001170653718121636
46	8.361812272297399E-4
47	0.00209045306807435
48	0.002592161804412194
49	5.01708736337844E-4
50	0.0013378899635675839
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1195913.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.11375407112107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.25513819674127	46.25954994163756
2	13.201969997919491	15.608360154226633
3	3.932376367398104	6.9737258849238
4	1.627780270344632	3.848968103319022
5	0.8276773756251885	2.446355841646915
6	0.5055583168636708	1.793127000701336
7	0.3236703116683017	1.3393357042858167
8	0.20870682250709022	0.9869955022919397
9	0.15608027606487188	0.8303841949186369
>10	0.8366337730937683	8.791092513753576
>50	0.06216539284809622	2.5692912227199867
>100	0.056972913675578815	6.3448338486153
>500	0.004842689148622469	1.9188478562312106
>1k	4.272961013490414E-4	0.2891322307283009
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1287	0.10761652394446752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.361812272297399E-5
2	0.0	0.0	0.0	0.0	8.361812272297399E-5
3	0.0	0.0	0.0	0.0	8.361812272297399E-5
4	0.0	0.0	0.0	0.0	8.361812272297399E-5
5	0.0	0.0	0.0	0.0	8.361812272297399E-5
6	0.0	0.0	0.0	0.0	8.361812272297399E-5
7	0.0	0.0	0.0	0.0	8.361812272297399E-5
8	0.0	0.0	0.0	1.6723624544594799E-4	8.361812272297399E-5
9	0.0	0.0	0.0	2.50854368168922E-4	8.361812272297399E-5
10	0.0	0.0	0.0	2.50854368168922E-4	1.6723624544594799E-4
11	0.0	0.0	0.0	2.50854368168922E-4	1.6723624544594799E-4
12	0.0	0.0	0.0	2.50854368168922E-4	3.3447249089189597E-4
13	0.0	0.0	0.0	2.50854368168922E-4	3.3447249089189597E-4
14	0.0	0.0	0.0	2.50854368168922E-4	3.3447249089189597E-4
15	0.0	0.0	0.0	2.50854368168922E-4	4.1809061361487E-4
16	0.0	0.0	0.0	2.50854368168922E-4	5.01708736337844E-4
17	0.0	0.0	8.361812272297399E-5	3.3447249089189597E-4	5.01708736337844E-4
18	0.0	0.0	8.361812272297399E-5	4.1809061361487E-4	5.01708736337844E-4
19	0.0	0.0	8.361812272297399E-5	5.01708736337844E-4	5.01708736337844E-4
20	0.0	0.0	8.361812272297399E-5	6.689449817837919E-4	5.01708736337844E-4
21	0.0	0.0	8.361812272297399E-5	8.3618122722974E-4	5.01708736337844E-4
22	0.0	0.0	8.361812272297399E-5	0.0013378899635675839	5.01708736337844E-4
23	0.0	0.0	8.361812272297399E-5	0.00250854368168922	5.01708736337844E-4
24	0.0	0.0	8.361812272297399E-5	0.004515378627040596	5.01708736337844E-4
25	0.0	0.0	8.361812272297399E-5	0.005267941731547362	5.01708736337844E-4
26	0.0	0.0	8.361812272297399E-5	0.006104122958777102	5.01708736337844E-4
27	0.0	0.0	8.361812272297399E-5	0.007525631045067659	5.85326859060818E-4
28	0.0	0.0	8.361812272297399E-5	0.011706537181216359	5.85326859060818E-4
29	0.0	0.0	8.361812272297399E-5	0.020820912558020526	5.85326859060818E-4
30	0.0	0.0	8.361812272297399E-5	0.03829710020712209	5.85326859060818E-4
31	0.0	0.0	8.361812272297399E-5	0.0742528929780009	7.525631045067659E-4
32	0.0	0.0	8.361812272297399E-5	0.11321893816690679	7.525631045067659E-4
33	0.0	0.0	8.361812272297399E-5	0.14942558530595454	7.525631045067659E-4
34	0.0	0.0	8.361812272297399E-5	0.18663564991767795	8.3618122722974E-4
35	0.0	0.0	8.361812272297399E-5	0.22727405756104332	8.3618122722974E-4
36	0.0	0.0	8.361812272297399E-5	0.28413438101266564	8.3618122722974E-4
37	0.0	0.0	8.361812272297399E-5	0.3739402448171397	8.3618122722974E-4
38	0.0	0.0	8.361812272297399E-5	0.49819677518347905	8.3618122722974E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAAT	90	4.0857958E-7	21.99772	3
TAGGACC	920	0.0	20.802193	4
TTAGGAC	1020	0.0	20.70374	3
GTCCTAC	1480	0.0	20.512247	1
GTGTTAT	110	1.266435E-7	19.998766	1
TCCTACA	1660	0.0	19.214876	2
TGTAGGA	1845	0.0	18.838156	2
TAGGACA	585	0.0	18.801472	4
GTCCTAA	735	0.0	18.556679	1
ATCGTAA	120	3.1639502E-7	18.332201	1
GCACTAC	60	0.0074164863	18.331434	6
TGCGTAC	60	0.007418275	18.33067	16
GTAGGAC	1815	0.0	18.179935	3
CCTACAG	1720	0.0	18.16091	3
TTTCGCC	85	1.4314934E-4	18.116528	43
GTATCAA	3165	0.0	18.071552	1
CTGTAGG	1885	0.0	17.972364	1
TAGGACG	1775	0.0	17.96997	4
TTTAGGA	1165	0.0	17.749235	2
GACGTGA	1030	0.0	17.72706	7
>>END_MODULE