FastQCFastQC Report
Thu 2 Feb 2017
SRR4064017_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064017_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641967
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13890.21636626181719623No Hit
TATCAACGCAGAGTACTTTTTTTTT8900.13863640965968657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA402.7590676E-416.6241139
ATACGCT753.272362E-916.4657888
GCGTAAC350.002166845416.28738611
GTACTAG350.00217249516.2810381
GGTATCA2000.015.6704991
ACGCTAT807.690687E-915.43667710
TACGCTA851.7114871E-814.5286379
ATATACG851.7167622E-814.5252416
ATAGCGT400.005284690314.2459096
TAGACTG602.5705773E-514.2459085
GTATCAA8300.013.9598451
ATCGCCA551.9517475E-413.82283316
CGCGGTC551.9582141E-413.81744510
ACCGTCG551.9582141E-413.8174458
CATTTAC551.962103E-413.8142141
TTAGACT551.9634009E-413.8131384
CTAGATA551.9646993E-413.8120613
GTTACAC551.9646993E-413.8120613
GTCTTAG707.2681814E-613.5675331
ACAGTAC500.001498475913.299298
Produced by FastQC (version 0.11.5)