##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064017_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	641967
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21064322620945	32.0	32.0	32.0	32.0	32.0
2	30.818031144903088	32.0	32.0	32.0	32.0	32.0
3	30.844692951506854	32.0	32.0	32.0	32.0	32.0
4	30.87960907647901	32.0	32.0	32.0	32.0	32.0
5	30.794140508780046	32.0	32.0	32.0	32.0	32.0
6	34.429561021049366	36.0	36.0	36.0	32.0	36.0
7	34.37579813292584	36.0	36.0	36.0	32.0	36.0
8	34.32741869909201	36.0	36.0	36.0	32.0	36.0
9	34.45824162301177	36.0	36.0	36.0	32.0	36.0
10	34.18446586818325	36.0	36.0	36.0	32.0	36.0
11	34.443955841966954	36.0	36.0	36.0	32.0	36.0
12	34.27066811845469	36.0	36.0	36.0	32.0	36.0
13	34.34264222304262	36.0	36.0	36.0	32.0	36.0
14	34.25374824562633	36.0	36.0	36.0	32.0	36.0
15	34.18672766668692	36.0	36.0	36.0	32.0	36.0
16	34.18356395266424	36.0	36.0	36.0	32.0	36.0
17	34.11602465547295	36.0	36.0	36.0	32.0	36.0
18	34.11252759098209	36.0	36.0	36.0	32.0	36.0
19	34.12774332637036	36.0	36.0	36.0	32.0	36.0
20	34.1127519015775	36.0	36.0	36.0	32.0	36.0
21	34.10630608738455	36.0	36.0	36.0	32.0	36.0
22	34.07935143083679	36.0	36.0	36.0	32.0	36.0
23	34.01137130101703	36.0	36.0	36.0	32.0	36.0
24	34.00507970035843	36.0	36.0	36.0	32.0	36.0
25	33.59383114708388	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	4.0
5	6.0
6	22.0
7	15.0
8	31.0
9	41.0
10	63.0
11	12.0
12	47.0
13	32.0
14	48.0
15	100.0
16	171.0
17	239.0
18	309.0
19	421.0
20	645.0
21	933.0
22	1451.0
23	2299.0
24	3480.0
25	5115.0
26	7207.0
27	9725.0
28	13267.0
29	17795.0
30	23562.0
31	31666.0
32	44190.0
33	61713.0
34	130956.0
35	286399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.99715601405631	17.226607241645304	11.41599333026858	26.36024341402981
2	16.78649042318687	19.457560580450682	37.49842268836217	26.25752630800028
3	18.964880715938033	23.54277168960441	28.296843236675468	29.195504357782088
4	12.712304013583719	15.326702443355739	35.84123250434227	36.119761038718266
5	14.594763849813527	36.24365577126193	33.775754686706485	15.385825692218058
6	34.15543808976319	35.4837051265873	16.774551363241933	13.58630542040758
7	30.182667468550473	30.20401295063117	20.860924056825723	18.752395523992632
8	27.916177760740734	32.91743216478974	19.255335892005938	19.911054182463587
9	27.26782530638706	14.4734381428518	18.487794387163536	39.7709421635976
10	15.735473353137555	26.868802738094864	31.74011165591773	25.655612252849846
11	37.03779322125298	21.29131904560778	22.26478788946157	19.406099843677673
12	24.491828575436628	23.642343332320188	28.999399904919997	22.866428187323187
13	29.443607944360796	19.814764590172114	24.932586406302118	25.80904105916498
14	23.4980539349171	19.453168960846448	25.52018467744575	31.5285924267907
15	25.21668653901605	26.884716994365604	22.378291601399635	25.520304865218712
16	25.56565247209926	25.856506016584575	23.593896128187545	24.983945383128624
17	24.024826287684427	25.83053034860921	25.02361365258784	25.121029711118524
18	24.62006600227906	25.243611387809377	25.770663124928877	24.36565948498269
19	25.286395939561064	25.117886476686923	25.40174931840183	24.193968265350183
20	25.774026747396267	24.508677548742522	24.900270155249633	24.817025548611575
21	26.425203233022348	24.49521048160186	24.60448476629177	24.475101519084028
22	25.93463261634777	24.318842929341745	24.97373270021606	24.772791754094424
23	24.682054431907112	24.464582469421416	25.442037096507665	25.411326002163808
24	24.7589667487128	24.811655019648985	25.19886704629241	25.2305111853458
25	24.682762878112353	24.618536001346893	25.30788369323248	25.39081742730827
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	22.0
2	86.0
3	150.0
4	150.0
5	150.0
6	449.5
7	749.0
8	749.0
9	749.0
10	870.0
11	991.0
12	991.0
13	991.0
14	1346.5
15	1702.0
16	1702.0
17	1702.0
18	2891.0
19	4080.0
20	4080.0
21	4080.0
22	6197.5
23	8315.0
24	8315.0
25	8315.0
26	12684.0
27	17053.0
28	17053.0
29	17053.0
30	21610.0
31	26167.0
32	26167.0
33	26167.0
34	32749.0
35	39331.0
36	39331.0
37	39331.0
38	46141.0
39	52951.0
40	52951.0
41	52951.0
42	60797.5
43	68644.0
44	68644.0
45	68644.0
46	76333.0
47	84022.0
48	84022.0
49	84022.0
50	86534.5
51	89047.0
52	89047.0
53	89047.0
54	84132.0
55	79217.0
56	79217.0
57	79217.0
58	72549.5
59	65882.0
60	65882.0
61	65882.0
62	56030.5
63	46179.0
64	46179.0
65	46179.0
66	37547.5
67	28916.0
68	28916.0
69	28916.0
70	21825.5
71	14735.0
72	14735.0
73	14735.0
74	11002.0
75	7269.0
76	7269.0
77	7269.0
78	5547.0
79	3825.0
80	3825.0
81	3825.0
82	2576.5
83	1328.0
84	1328.0
85	1328.0
86	1008.5
87	689.0
88	689.0
89	689.0
90	478.5
91	268.0
92	268.0
93	268.0
94	206.5
95	145.0
96	145.0
97	145.0
98	217.5
99	290.0
100	290.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040812066663862785
2	0.008100104834049103
3	0.001869254961703639
4	0.0034269674297900047
5	0.007009706106388647
6	0.012150157251073653
7	0.022586830787252304
8	0.035515844272369146
9	0.04595251780854779
10	0.05498725012344872
11	0.05358530890217098
12	0.0626200412170719
13	0.06308735495749782
14	0.06480083867239282
15	0.057946903812812804
16	0.06339889745111509
17	0.06059501500855963
18	0.07383557098729374
19	0.07165477353197283
20	0.07539328345538011
21	0.0719663160255901
22	0.07554905470218874
23	0.07866447963836147
24	0.07134323103835555
25	0.07648368218304055
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	641967.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.18212788042677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8926769108329	39.67190890959787
2	15.80297191773257	17.440832345102233
3	5.6881533922346375	9.416532236813252
4	2.5180771097025505	5.558114123215263
5	1.2724547478288797	3.510838030837472
6	0.7234700101335664	2.395356877010647
7	0.4577175304860918	1.7680479110277658
8	0.3184273166963486	1.4057197528447252
9	0.2214374752330379	1.09974519682455
>10	0.9757833910693446	9.602101152566288
>50	0.08069588041423874	3.0739548456528034
>100	0.04756803154595904	4.700767728609654
>500	2.8314304491455436E-4	0.1390574778450624
>1k	2.8314304491455436E-4	0.21702341205257492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1389	0.21636626181719623	No Hit
TATCAACGCAGAGTACTTTTTTTTT	890	0.13863640965968657	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	40	2.7590676E-4	16.624113	9
ATACGCT	75	3.272362E-9	16.465788	8
GCGTAAC	35	0.0021668454	16.287386	11
GTACTAG	35	0.002172495	16.281038	1
GGTATCA	200	0.0	15.670499	1
ACGCTAT	80	7.690687E-9	15.436677	10
TACGCTA	85	1.7114871E-8	14.528637	9
ATATACG	85	1.7167622E-8	14.525241	6
ATAGCGT	40	0.0052846903	14.245909	6
TAGACTG	60	2.5705773E-5	14.245908	5
GTATCAA	830	0.0	13.959845	1
ATCGCCA	55	1.9517475E-4	13.822833	16
CGCGGTC	55	1.9582141E-4	13.817445	10
ACCGTCG	55	1.9582141E-4	13.817445	8
CATTTAC	55	1.962103E-4	13.814214	1
TTAGACT	55	1.9634009E-4	13.813138	4
CTAGATA	55	1.9646993E-4	13.812061	3
GTTACAC	55	1.9646993E-4	13.812061	3
GTCTTAG	70	7.2681814E-6	13.567533	1
ACAGTAC	50	0.0014984759	13.29929	8
>>END_MODULE