FastQCFastQC Report
Thu 2 Feb 2017
SRR4064017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641967
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8000.028.0486091
ACCGTCG1550.026.9664068
CGTCGTA1650.025.33207710
ATACCGT1900.024.3145836
GTCGTAG1750.023.8845311
TACCGTC1750.023.884537
CCGTCGT1800.023.2210719
ATTAGGC500.00257987521.9989093
TCCGACC2000.021.99890919
GGTATCA3400.021.9989091
GACCATA2000.020.89896422
CTTATAT755.4696E-520.5323163
TAACGGC550.004478607320.00212336
CCCGTTG550.004482647419.99900842
TTCCGAC2150.019.44089718
CGATGCG2200.018.99905844
ATAAACG2350.018.72539326
ATAAGAC1303.58159E-818.6144623
CGACCAT2250.018.57685721
TAAACGA2400.018.3352827
Produced by FastQC (version 0.11.5)