##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064017_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	641967
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.326854807178563	32.0	32.0	32.0	32.0	32.0
2	31.369251067422468	32.0	32.0	32.0	32.0	32.0
3	31.446008906999893	32.0	32.0	32.0	32.0	32.0
4	31.527073821551575	32.0	32.0	32.0	32.0	32.0
5	31.4869066478495	32.0	32.0	32.0	32.0	32.0
6	35.057487378634725	36.0	36.0	36.0	36.0	36.0
7	35.074793564155165	36.0	36.0	36.0	36.0	36.0
8	35.02595927828066	36.0	36.0	36.0	36.0	36.0
9	35.13571102564462	36.0	36.0	36.0	36.0	36.0
10	34.986122339621815	36.0	36.0	36.0	36.0	36.0
11	35.1274582649887	36.0	36.0	36.0	36.0	36.0
12	35.034707391501435	36.0	36.0	36.0	36.0	36.0
13	35.07582321209657	36.0	36.0	36.0	36.0	36.0
14	35.01888259053814	36.0	36.0	36.0	36.0	36.0
15	34.982983548998625	36.0	36.0	36.0	36.0	36.0
16	35.012052021365584	36.0	36.0	36.0	36.0	36.0
17	34.97042527108091	36.0	36.0	36.0	36.0	36.0
18	34.96711980522363	36.0	36.0	36.0	36.0	36.0
19	34.96401061113733	36.0	36.0	36.0	36.0	36.0
20	34.96303392541984	36.0	36.0	36.0	36.0	36.0
21	34.952871409278046	36.0	36.0	36.0	36.0	36.0
22	34.94841167848192	36.0	36.0	36.0	36.0	36.0
23	34.89666291257962	36.0	36.0	36.0	32.0	36.0
24	34.87278162273139	36.0	36.0	36.0	32.0	36.0
25	34.83838110058617	36.0	36.0	36.0	32.0	36.0
26	34.78753580791536	36.0	36.0	36.0	32.0	36.0
27	34.773260930857816	36.0	36.0	36.0	32.0	36.0
28	34.75871345411836	36.0	36.0	36.0	32.0	36.0
29	34.73677307400536	36.0	36.0	36.0	32.0	36.0
30	34.72474286061433	36.0	36.0	36.0	32.0	36.0
31	34.70981530203266	36.0	36.0	36.0	32.0	36.0
32	34.6776033659051	36.0	36.0	36.0	32.0	36.0
33	34.66409176795692	36.0	36.0	36.0	32.0	36.0
34	34.64538519892767	36.0	36.0	36.0	32.0	36.0
35	34.61207351779765	36.0	36.0	36.0	32.0	36.0
36	34.609297674179516	36.0	36.0	36.0	32.0	36.0
37	34.589603515445496	36.0	36.0	36.0	32.0	36.0
38	34.55227605157275	36.0	36.0	36.0	32.0	36.0
39	34.55994311234067	36.0	36.0	36.0	32.0	36.0
40	34.536538482507666	36.0	36.0	36.0	32.0	36.0
41	34.51521028339463	36.0	36.0	36.0	32.0	36.0
42	34.46613610980004	36.0	36.0	36.0	32.0	36.0
43	34.459861643978584	36.0	36.0	36.0	32.0	36.0
44	34.40626854651407	36.0	36.0	36.0	32.0	36.0
45	34.37151598135107	36.0	36.0	36.0	32.0	36.0
46	34.35682052192714	36.0	36.0	36.0	32.0	36.0
47	34.320089661929664	36.0	36.0	36.0	32.0	36.0
48	34.29799662599479	36.0	36.0	36.0	32.0	36.0
49	34.27341592324839	36.0	36.0	36.0	32.0	36.0
50	33.68607732173149	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	19.0
22	52.0
23	176.0
24	479.0
25	1157.0
26	2323.0
27	4347.0
28	7351.0
29	11370.0
30	16435.0
31	23499.0
32	33528.0
33	54190.0
34	112217.0
35	374819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.212419089754874	18.16564498414899	11.824151172663047	26.797784753433085
2	16.15375987986828	20.193562858860805	36.903741966035376	26.748935295235544
3	18.25844359358595	24.047067913253972	28.57300853939305	29.12147995376702
4	12.484909660465414	16.067648337064057	35.845767773109834	35.6016742293607
5	14.27908288120729	36.663255276361554	33.56683443229948	15.490827410131674
6	34.20659102379573	35.846246830040315	16.60482519268993	13.342336953474026
7	30.216817998432944	30.419009076790555	20.70994303445504	18.654229890321467
8	28.011875999856688	33.056060514013964	19.155813305045275	19.776250181084073
9	27.391355820785783	14.333336448801948	18.409771355758476	39.86553637465379
10	15.726115483111203	27.10681595852727	31.738072539893203	25.428996018468325
11	37.2087661826854	21.26885026800443	22.04817381578804	19.474209733522127
12	24.47913989348362	23.737045673687277	28.853196503870137	22.930617928958966
13	29.423988186290238	19.88672297286772	24.923749855911375	25.765538984930664
14	23.535047027412666	19.454924404096165	25.428605253237713	31.581423315253453
15	25.1939421507172	26.914029884226657	22.346964844285484	25.54506312077066
16	25.85947454833775	25.880503830444795	23.46914614883747	24.790875472379987
17	24.238473317164278	25.82391306718258	25.020444976143636	24.91716863950951
18	24.66034026724157	25.370969802139054	25.672854948704448	24.29583498191493
19	25.43697729010993	25.0397606107479	25.357689725484335	24.165572373657838
20	25.924391281117448	24.58521752811299	24.76747102247326	24.7229201682963
21	26.677851042187527	24.35483443853033	24.634288055305024	24.33302646397712
22	25.95499754659522	24.24462392809577	24.89092084459433	24.90945768071468
23	24.78351421196681	24.551256388647875	25.192107284110044	25.473122115275277
24	24.98426703055	24.7895520621094	25.111532733922775	25.114648173417827
25	24.9511261622627	24.63381617829176	25.177308831249345	25.237748828196192
26	24.901156831161224	25.150095182310732	25.34918355075196	24.599564435776085
27	25.150054133206634	24.849244861239843	24.78475235031584	25.21594865523768
28	24.736971645590128	24.73292146068361	25.439990279556223	25.09011661417004
29	24.715205007220153	25.126605841860698	25.315248935669743	24.842940215249403
30	24.89278743326962	24.97924289935805	25.356842989084804	24.771126678287526
31	24.900844650907743	24.862366455737615	25.002258841416868	25.234530051937774
32	24.96335401501356	24.792469230613456	24.594323883524183	25.6498528708488
33	24.607401614790145	24.706943472998297	25.10666868138972	25.578986230821837
34	25.20169047696935	24.777980805328774	25.25262910293497	24.767699614766904
35	25.51028196943994	24.540890319947533	25.274593464589866	24.674234246022657
36	24.779260108547323	24.822099537022613	25.192387978789032	25.206252375641036
37	25.43777251251279	24.824478185757926	24.81949331950033	24.918255982228953
38	24.874204332667617	24.725274725274726	25.152746318042603	25.247774624015058
39	25.319381938894885	24.83164393100322	24.7934785487185	25.05549558138339
40	25.67567146555672	24.848509394754142	24.800063555955724	24.675755583733412
41	24.703052734718803	25.079095068299818	25.371797448083882	24.846054748897497
42	25.657869116251973	25.016862447601795	24.852210187878043	24.47305824826819
43	25.213096061461393	24.410870391486128	25.180383946763367	25.195649600289112
44	24.718791328445185	24.9938859214189	24.964133335202607	25.323189414933307
45	25.00802233167902	24.96985784116718	24.97546568467096	25.046654142482843
46	24.85665017033745	24.865061896283418	24.921140069256545	25.357147864122588
47	24.86151612581626	24.836280594187727	25.284444942924083	25.017758337071932
48	25.401117833620994	25.10296655845572	24.60604776651359	24.88986784140969
49	25.009073731663662	25.10518518980066	24.63101456002916	25.25472651850652
50	25.083572445549397	25.24214979796186	24.816732558827837	24.857545197660908
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	9.5
2	16.0
3	39.0
4	62.0
5	69.5
6	77.0
7	74.0
8	71.0
9	109.5
10	148.0
11	212.5
12	277.0
13	488.0
14	699.0
15	958.5
16	1218.0
17	1219.0
18	1220.0
19	1276.0
20	1332.0
21	1549.0
22	1766.0
23	2143.0
24	2520.0
25	3149.5
26	3779.0
27	4538.5
28	5298.0
29	6199.5
30	7101.0
31	8271.5
32	9442.0
33	10930.0
34	12418.0
35	14405.5
36	16393.0
37	19232.0
38	22071.0
39	25025.0
40	27979.0
41	31013.5
42	34048.0
43	35393.5
44	36739.0
45	39653.0
46	42567.0
47	45239.5
48	47912.0
49	49829.0
50	51746.0
51	51612.0
52	51478.0
53	50663.0
54	49848.0
55	48852.5
56	47857.0
57	45777.0
58	43697.0
59	40074.5
60	36452.0
61	31915.5
62	27379.0
63	23601.5
64	19824.0
65	16576.0
66	13328.0
67	11203.0
68	9078.0
69	7826.0
70	6574.0
71	5083.0
72	3592.0
73	2986.5
74	2381.0
75	1866.5
76	1352.0
77	1131.0
78	910.0
79	760.0
80	610.0
81	473.5
82	337.0
83	258.5
84	180.0
85	139.0
86	98.0
87	68.0
88	38.0
89	29.5
90	21.0
91	19.0
92	17.0
93	12.0
94	7.0
95	5.5
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008100104834049103
2	7.788562340431829E-4
3	1.557712468086366E-4
4	0.0
5	0.0
6	4.6731374042590973E-4
7	0.0
8	0.0
9	0.001401941221277729
10	4.6731374042590973E-4
11	0.0
12	0.0
13	1.557712468086366E-4
14	1.557712468086366E-4
15	0.003582738676598641
16	7.788562340431829E-4
17	0.0
18	1.557712468086366E-4
19	0.0
20	6.230849872345464E-4
21	0.0
22	3.115424936172732E-4
23	0.0012461699744690928
24	4.6731374042590973E-4
25	0.0012461699744690928
26	0.006386621119154099
27	0.004984679897876371
28	0.0032711961829813684
29	0.002180797455320912
30	0.002803882442555458
31	0.007009706106388647
32	0.002180797455320912
33	0.004050052417024551
34	0.002803882442555458
35	0.002803882442555458
36	0.00545199363830228
37	0.003738509923407278
38	0.008255876080857738
39	0.003738509923407278
40	0.0020250262085122757
41	0.002803882442555458
42	0.0012461699744690928
43	4.6731374042590973E-4
44	9.346274808518195E-4
45	0.001401941221277729
46	9.346274808518195E-4
47	0.0023365687021295488
48	0.0017134837148950024
49	6.230849872345464E-4
50	0.001401941221277729
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	641967.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.596334654799584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6303415604044	41.8324145860545
2	15.596237865988948	17.965722709707137
3	5.587263647293642	9.654177205023622
4	2.4024632336302374	5.534923056000764
5	1.2317957633723242	3.5473460506778354
6	0.68591731739225	2.3703794014827846
7	0.43409806039994525	1.7501720011856254
8	0.2857861526575181	1.3168187910536044
9	0.1931794084483327	1.0013783271665748
>10	0.8530478319055746	8.676951808846704
>50	0.06367683820255013	2.543619025981074
>100	0.03619232030425289	3.806097036819936
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	1.557712468086366E-4	0.0
18	0.0	0.0	0.0	1.557712468086366E-4	0.0
19	0.0	0.0	0.0	1.557712468086366E-4	0.0
20	0.0	0.0	0.0	1.557712468086366E-4	0.0
21	0.0	0.0	0.0	1.557712468086366E-4	0.0
22	0.0	0.0	0.0	1.557712468086366E-4	0.0
23	0.0	0.0	0.0	1.557712468086366E-4	0.0
24	0.0	0.0	0.0	1.557712468086366E-4	0.0
25	0.0	0.0	0.0	1.557712468086366E-4	0.0
26	0.0	0.0	0.0	1.557712468086366E-4	0.0
27	0.0	0.0	0.0	1.557712468086366E-4	0.0
28	0.0	0.0	0.0	9.346274808518195E-4	0.0
29	0.0	0.0	0.0	0.001869254961703639	0.0
30	0.0	0.0	0.0	0.004050052417024551	0.0
31	0.0	0.0	0.0	0.011994386004265016	0.0
32	0.0	0.0	0.0	0.020406033331931393	0.0
33	0.0	0.0	0.0	0.028661909412789132	0.0
34	0.0	0.0	0.0	0.03785241297449869	0.0
35	0.0	0.0	0.0	0.05233913892770189	0.0
36	0.0	0.0	0.0	0.07056437480431237	0.0
37	0.0	0.0	0.0	0.10327633663412605	0.0
38	0.0	0.0	0.0	0.1591982142384266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	800	0.0	28.048609	1
ACCGTCG	155	0.0	26.966406	8
CGTCGTA	165	0.0	25.332077	10
ATACCGT	190	0.0	24.314583	6
GTCGTAG	175	0.0	23.88453	11
TACCGTC	175	0.0	23.88453	7
CCGTCGT	180	0.0	23.221071	9
ATTAGGC	50	0.002579875	21.998909	3
TCCGACC	200	0.0	21.998909	19
GGTATCA	340	0.0	21.998909	1
GACCATA	200	0.0	20.898964	22
CTTATAT	75	5.4696E-5	20.532316	3
TAACGGC	55	0.0044786073	20.002123	36
CCCGTTG	55	0.0044826474	19.999008	42
TTCCGAC	215	0.0	19.440897	18
CGATGCG	220	0.0	18.999058	44
ATAAACG	235	0.0	18.725393	26
ATAAGAC	130	3.58159E-8	18.614462	3
CGACCAT	225	0.0	18.576857	21
TAAACGA	240	0.0	18.33528	27
>>END_MODULE