FastQCFastQC Report
Thu 2 Feb 2017
SRR4064016_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064016_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527289
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48940.19364623515553622No Hit
TATCAACGCAGAGTACTTTTTTTTT32900.13017901791207892No Hit
GCGCAAGACGGACCAGAGCGAAAGC27830.1101179960028315No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG25830.10220437789267471No Hit
GTCTTGCGCCGGTCCAAGAATTTCA25360.10034467763678787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGT2950.014.1705099
GGTATCA9000.013.7177751
GCGTTAT1850.013.3470251
CGTTATT1650.013.2344652
CGTCGTA3300.012.95522110
GTATCAA27200.012.6392841
ACCGTCG3450.012.3926878
TAGGACG1001.4495345E-712.3462434
CGCGGTC1700.012.29475910
AACCGCG1800.012.1392817
ATACCGT4200.011.9868216
CGAACGA1800.011.61378616
ACGAACG2050.011.58780615
TACCGTC3800.011.5003727
AGCGGAA752.0711211E-411.40217512
CCGGTCC4500.011.4008219
ATCGCCA3800.011.25259216
ACGGTAT4100.011.1227529
CGCATCG3800.011.00166213
TAACGAA1900.011.00166213
Produced by FastQC (version 0.11.5)