##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064016_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2527289
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18055948488677	32.0	32.0	32.0	32.0	32.0
2	30.741229831649644	32.0	32.0	32.0	32.0	32.0
3	30.76692218420608	32.0	32.0	32.0	32.0	32.0
4	30.74685720548778	32.0	32.0	32.0	32.0	32.0
5	30.703681296440575	32.0	32.0	32.0	32.0	32.0
6	34.119712862280494	36.0	36.0	36.0	32.0	36.0
7	34.11455872280535	36.0	36.0	36.0	32.0	36.0
8	34.073212837946116	36.0	36.0	36.0	32.0	36.0
9	34.28223839853693	36.0	36.0	36.0	32.0	36.0
10	33.876297091468366	36.0	36.0	36.0	32.0	36.0
11	34.24504914159006	36.0	36.0	36.0	32.0	36.0
12	34.021314143336994	36.0	36.0	36.0	32.0	36.0
13	34.17113001322761	36.0	36.0	36.0	32.0	36.0
14	34.00685715009245	36.0	36.0	36.0	32.0	36.0
15	33.92297517221022	36.0	36.0	36.0	32.0	36.0
16	33.92568321232752	36.0	36.0	36.0	32.0	36.0
17	33.846879798867484	36.0	36.0	36.0	32.0	36.0
18	33.848836045264314	36.0	36.0	36.0	32.0	36.0
19	33.84269863873898	36.0	36.0	36.0	32.0	36.0
20	33.826625288995444	36.0	36.0	36.0	32.0	36.0
21	33.792907736313495	36.0	36.0	36.0	32.0	36.0
22	33.777907077504786	36.0	36.0	36.0	32.0	36.0
23	33.73119575956687	36.0	36.0	36.0	32.0	36.0
24	33.70210015554216	36.0	36.0	36.0	32.0	36.0
25	33.022339748244065	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	24.0
5	35.0
6	107.0
7	41.0
8	123.0
9	167.0
10	217.0
11	79.0
12	164.0
13	128.0
14	407.0
15	863.0
16	1344.0
17	1743.0
18	2103.0
19	2660.0
20	3422.0
21	4582.0
22	6149.0
23	8687.0
24	12501.0
25	18076.0
26	25933.0
27	36477.0
28	51904.0
29	73243.0
30	103098.0
31	152215.0
32	231445.0
33	341456.0
34	668142.0
35	779746.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.727475676659886	17.269611064052125	11.521386331430742	26.481526927857253
2	16.86451737485349	19.280076671106436	36.96120305893023	26.894202895109842
3	18.60449840913974	23.005410230650007	28.719647331940777	29.670444028269472
4	12.695463203970242	15.026262245756374	35.4481754921055	36.83009905816789
5	14.851216374449509	35.94352169854365	33.743256489064954	15.462005437941892
6	35.04821204276123	35.20860226627864	16.20143080901098	13.541754881949156
7	30.574621753156162	30.147665573155425	20.674644589058637	18.603068084629776
8	28.2738324951958	32.82095126136235	19.027008413040512	19.878207830401337
9	26.970081802375844	14.057348754279344	18.46455863770383	40.50801080564098
10	15.764727680291132	26.375039441808834	31.511283425572067	26.348949452327968
11	37.41707604913619	20.941432541066582	22.194184466177006	19.447306943620223
12	24.45831502179568	23.37427199265163	28.66375265764749	23.503660327905198
13	29.141919588046015	19.10679691604648	25.400846254076566	26.35043724183094
14	23.45346813771586	19.36518934842129	24.786360657814768	32.394981856048084
15	25.01389645517661	27.07508519991892	21.999751368266356	25.91126697663812
16	25.89921790956607	25.748802197597705	23.277375837749	25.074604055087228
17	24.109655209275715	25.965115105108016	25.017588939457227	24.90764074615904
18	24.954275131650796	24.926517493449634	25.656095929764067	24.463111445135503
19	25.448193446611167	24.901483762243256	25.21560326148934	24.43471952965624
20	25.59612952378539	24.282185529747277	24.73918714614152	25.382497800325815
21	26.563736773269795	24.10669209292675	24.541145361680762	24.78842577212269
22	25.88319007657739	24.002353668836733	24.857300907294988	25.257155347290883
23	24.433609156658566	24.024903298158467	25.46774862906726	26.07373891611571
24	24.8399965946191	24.838293904170207	24.96262990439195	25.35907959681874
25	24.77430370629368	24.436298782730862	25.099795795291612	25.689601715683846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	96.0
1	96.0
2	310.5
3	525.0
4	525.0
5	525.0
6	1548.5
7	2572.0
8	2572.0
9	2572.0
10	3182.0
11	3792.0
12	3792.0
13	3792.0
14	5110.5
15	6429.0
16	6429.0
17	6429.0
18	10872.5
19	15316.0
20	15316.0
21	15316.0
22	24078.5
23	32841.0
24	32841.0
25	32841.0
26	49063.0
27	65285.0
28	65285.0
29	65285.0
30	81320.0
31	97355.0
32	97355.0
33	97355.0
34	124360.0
35	151365.0
36	151365.0
37	151365.0
38	177321.5
39	203278.0
40	203278.0
41	203278.0
42	234489.0
43	265700.0
44	265700.0
45	265700.0
46	296823.0
47	327946.0
48	327946.0
49	327946.0
50	337354.5
51	346763.0
52	346763.0
53	346763.0
54	327188.5
55	307614.0
56	307614.0
57	307614.0
58	282906.0
59	258198.0
60	258198.0
61	258198.0
62	225203.0
63	192208.0
64	192208.0
65	192208.0
66	157159.0
67	122110.0
68	122110.0
69	122110.0
70	91958.5
71	61807.0
72	61807.0
73	61807.0
74	47173.0
75	32539.0
76	32539.0
77	32539.0
78	26393.0
79	20247.0
80	20247.0
81	20247.0
82	13647.5
83	7048.0
84	7048.0
85	7048.0
86	5269.5
87	3491.0
88	3491.0
89	3491.0
90	2409.5
91	1328.0
92	1328.0
93	1328.0
94	854.0
95	380.0
96	380.0
97	380.0
98	718.0
99	1056.0
100	1056.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03596739431066253
2	0.005183419862152687
3	0.0015827236220313547
4	0.003442423877918196
5	0.009100660826680288
6	0.012938765610106323
7	0.022395539251743667
8	0.03379114933036942
9	0.04752127675149142
10	0.05602841621990995
11	0.052625560432542545
12	0.06168665316867204
13	0.062121902164730666
14	0.06544562177099651
15	0.05796725265689835
16	0.06417944287337142
17	0.06148881271591813
18	0.07351751224335641
19	0.07189522053077428
20	0.0757728934047511
21	0.07185565244022349
22	0.07399232932996583
23	0.07917574919211852
24	0.07415060169216896
25	0.07446714641657523
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2527289.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.91614160947309
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.97898746308572	36.40619922625595
2	12.994172527394749	12.452612218411431
3	4.357464034294212	6.263785911762822
4	1.936474340786997	3.7115351494504325
5	1.1499990337271853	2.7551758275414513
6	0.7248526580938721	2.0839285566737376
7	0.4776757188230186	1.602186417057143
8	0.3663517757370166	1.4043330852077451
9	0.2736762572951534	1.1802159269734647
>10	1.5496391805946141	13.360200106079775
>50	0.0995631852671352	3.3074599804798455
>100	0.07416787859823237	7.412547464109595
>500	0.011593329181916622	3.8447474772488777
>1k	0.0053826171201749986	4.215072652747667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4894	0.19364623515553622	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3290	0.13017901791207892	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2783	0.1101179960028315	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2583	0.10220437789267471	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2536	0.10034467763678787	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.956809055078386E-5
2	0.0	0.0	0.0	0.0	7.913618110156773E-5
3	0.0	0.0	0.0	0.0	7.913618110156773E-5
4	0.0	0.0	0.0	0.0	7.913618110156773E-5
5	0.0	0.0	0.0	0.0	7.913618110156773E-5
6	0.0	0.0	0.0	0.0	7.913618110156773E-5
7	0.0	0.0	0.0	3.956809055078386E-5	7.913618110156773E-5
8	0.0	0.0	0.0	3.956809055078386E-5	7.913618110156773E-5
9	0.0	0.0	0.0	3.956809055078386E-5	7.913618110156773E-5
10	0.0	0.0	0.0	3.956809055078386E-5	7.913618110156773E-5
11	0.0	0.0	0.0	3.956809055078386E-5	7.913618110156773E-5
12	0.0	0.0	0.0	3.956809055078386E-5	3.165447244062709E-4
13	0.0	0.0	0.0	3.956809055078386E-5	3.5611281495705476E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGT	295	0.0	14.170509	9
GGTATCA	900	0.0	13.717775	1
GCGTTAT	185	0.0	13.347025	1
CGTTATT	165	0.0	13.234465	2
CGTCGTA	330	0.0	12.955221	10
GTATCAA	2720	0.0	12.639284	1
ACCGTCG	345	0.0	12.392687	8
TAGGACG	100	1.4495345E-7	12.346243	4
CGCGGTC	170	0.0	12.294759	10
AACCGCG	180	0.0	12.139281	7
ATACCGT	420	0.0	11.986821	6
CGAACGA	180	0.0	11.613786	16
ACGAACG	205	0.0	11.587806	15
TACCGTC	380	0.0	11.500372	7
AGCGGAA	75	2.0711211E-4	11.402175	12
CCGGTCC	450	0.0	11.400821	9
ATCGCCA	380	0.0	11.252592	16
ACGGTAT	410	0.0	11.122752	9
CGCATCG	380	0.0	11.001662	13
TAACGAA	190	0.0	11.001662	13
>>END_MODULE