FastQCFastQC Report
Thu 2 Feb 2017
SRR4064016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527289
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT25480.1008194947233973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29850.026.9001671
GGTATCA13350.021.2576331
AACGCAG45800.017.1948456
TCAACGC45950.017.1387124
CAACGCA46400.017.0199055
TATCAAC47450.016.7363322
CGAGCCG7900.016.70757715
ATCAACG47400.016.7072473
ACCGTCG6000.016.4993848
TAACGGC2051.05501385E-1016.09950836
AGCGAAA8400.015.97464717
TACCGTC6900.015.9414337
GTATAGG3200.015.811911
ATACCGT7100.015.801296
GGCGTTA6000.015.76607842
CTAGCGG7400.015.75741529
ATAACGC8650.015.5129523
TCTAGCG7700.015.42952228
TAACGCC8700.015.4237974
TAATACT6150.015.3806274
Produced by FastQC (version 0.11.5)