##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064016_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2527289
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.151449636349465	32.0	32.0	32.0	32.0	32.0
2	30.910318131404836	32.0	32.0	32.0	32.0	32.0
3	31.18741149112745	32.0	32.0	32.0	32.0	32.0
4	31.36778500598863	32.0	32.0	32.0	32.0	32.0
5	31.20848070798393	32.0	32.0	32.0	32.0	32.0
6	34.82055752230948	36.0	36.0	36.0	32.0	36.0
7	34.85270936564833	36.0	36.0	36.0	32.0	36.0
8	34.765193850010824	36.0	36.0	36.0	32.0	36.0
9	34.98059066454213	36.0	36.0	36.0	32.0	36.0
10	34.71547733559557	36.0	36.0	36.0	32.0	36.0
11	34.97778805668841	36.0	36.0	36.0	36.0	36.0
12	34.8230590169941	36.0	36.0	36.0	32.0	36.0
13	34.91424209894476	36.0	36.0	36.0	32.0	36.0
14	34.8379227702095	36.0	36.0	36.0	32.0	36.0
15	34.77876491370793	36.0	36.0	36.0	32.0	36.0
16	34.80525456328896	36.0	36.0	36.0	32.0	36.0
17	34.75710613230224	36.0	36.0	36.0	32.0	36.0
18	34.76821883053343	36.0	36.0	36.0	32.0	36.0
19	34.76632431035786	36.0	36.0	36.0	32.0	36.0
20	34.76774559617044	36.0	36.0	36.0	32.0	36.0
21	34.76263695999943	36.0	36.0	36.0	32.0	36.0
22	34.745053691920475	36.0	36.0	36.0	32.0	36.0
23	34.68219898871874	36.0	36.0	36.0	32.0	36.0
24	34.65118591502594	36.0	36.0	36.0	32.0	36.0
25	34.61951917647725	36.0	36.0	36.0	32.0	36.0
26	34.542283846445734	36.0	36.0	36.0	32.0	36.0
27	34.54062950457981	36.0	36.0	36.0	32.0	36.0
28	34.51029977181082	36.0	36.0	36.0	32.0	36.0
29	34.481257980389266	36.0	36.0	36.0	32.0	36.0
30	34.462472238038465	36.0	36.0	36.0	32.0	36.0
31	34.465769447024066	36.0	36.0	36.0	32.0	36.0
32	34.422969039156186	36.0	36.0	36.0	32.0	36.0
33	34.394331633620055	36.0	36.0	36.0	32.0	36.0
34	34.38657153970124	36.0	36.0	36.0	32.0	36.0
35	34.35028760066617	36.0	36.0	36.0	32.0	36.0
36	34.31080893400003	36.0	36.0	36.0	32.0	36.0
37	34.31644857394623	36.0	36.0	36.0	32.0	36.0
38	34.27697109432281	36.0	36.0	36.0	32.0	36.0
39	34.27683695849584	36.0	36.0	36.0	32.0	36.0
40	34.25183150799137	36.0	36.0	36.0	32.0	36.0
41	34.23568060479035	36.0	36.0	36.0	32.0	36.0
42	34.157866393594084	36.0	36.0	36.0	32.0	36.0
43	34.16765356079182	36.0	36.0	36.0	32.0	36.0
44	34.086120740445594	36.0	36.0	36.0	32.0	36.0
45	34.0547563021087	36.0	36.0	36.0	32.0	36.0
46	34.0407472196492	36.0	36.0	36.0	32.0	36.0
47	34.03307773665774	36.0	36.0	36.0	32.0	36.0
48	33.974086461817386	36.0	36.0	36.0	32.0	36.0
49	33.95616805201146	36.0	36.0	36.0	32.0	36.0
50	33.22849701795086	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	3.0
20	17.0
21	62.0
22	227.0
23	714.0
24	1795.0
25	4393.0
26	9166.0
27	17307.0
28	30211.0
29	48915.0
30	75184.0
31	114103.0
32	176371.0
33	297050.0
34	581596.0
35	1170172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46171184004761	18.46734980721381	12.318253907956468	26.75268444478211
2	16.204502945658156	20.11657596964957	36.239766167486856	27.43915491720542
3	17.96099525102462	23.661546277779845	29.097583886562717	29.27987458463282
4	12.467944900642546	15.94653401332416	35.66117685789001	35.924344228143276
5	14.861695674693317	36.20207265571923	33.060801514983055	15.8754301546044
6	35.24452713305894	35.70636643419067	15.76992077667506	13.279185656075331
7	30.637782476710214	30.3673740388907	20.499681872426095	18.495161611972993
8	28.40399336997075	33.137682314923225	18.626718194872055	19.831606120233975
9	27.086269646756055	13.905720894786533	18.147228106939448	40.860781351517964
10	15.707846246862228	26.566155126951113	31.500368777084542	26.225629849102116
11	37.925737816292475	20.77233747307886	21.986128218814706	19.315796491813956
12	24.457945977600467	23.408717259826258	28.542526861829987	23.590809900743288
13	29.353643490970903	19.123925621044652	25.25155562871299	26.270875259271453
14	23.572804132187912	19.199210534958322	24.76907000052626	32.45891533232751
15	25.25664092903957	27.171787878056435	21.761108972134434	25.810462220769566
16	26.079720679277358	25.793562550004474	23.08910707742809	25.03760969329008
17	24.377742315975734	25.962246502081875	24.688747507704896	24.97126367423749
18	25.244086044905895	24.884530236993456	25.450394395566782	24.420989322533863
19	25.639183187669946	24.951014684515577	25.00209710962898	24.407705018185503
20	25.917635658531317	24.15277592576692	24.58379215940597	25.345796256295795
21	26.8645968070925	24.086560737612515	24.333346918377753	24.715495536917224
22	26.016129555578594	24.011449436272745	24.791060449826414	25.181360558322247
23	24.70154518695492	23.784190590072203	25.412985648132945	26.101278574839938
24	25.11908049833774	24.8314196832803	24.778714840686554	25.270784977695403
25	25.123780740417157	24.278910167608995	25.071352232128902	25.525956859844946
26	25.07022702550411	24.968531882218823	25.236540133374962	24.724700958902105
27	25.58443948060376	24.60405236698039	24.524002192179776	25.28750596023607
28	24.842740542570354	24.358646134268337	25.487804057703546	25.310809265457763
29	24.958947342597188	24.57758299363496	25.27775337700149	25.185716286766365
30	24.82205687532348	24.74418450054843	25.405506146694464	25.028252477433632
31	25.41888575972421	24.41414558305677	24.68021911099627	25.486749546222757
32	25.274720056789924	24.498567391933157	24.325609793254138	25.90110275802278
33	24.68760819802192	24.39000551995394	25.042982437049773	25.87940384497437
34	25.537397313339877	24.215197131084782	25.159868899786208	25.087536655789133
35	25.88937576516824	24.142991790986308	25.12307980858033	24.844552635265124
36	24.888459235733563	24.815846445514882	24.7934492579052	25.502245060846352
37	25.84513259103825	24.469582428348765	24.751474651660903	24.93381032895208
38	24.99128456249691	24.29693822030964	25.14972547855765	25.562051738635798
39	25.670758851286518	24.400288860090814	24.427394224776677	25.50155806384599
40	25.666182343081534	24.528852865343797	25.051558303221384	24.75340648835328
41	24.88187617940813	24.751693658765895	25.31112237513063	25.055307786695348
42	25.95890866270346	24.853002158870044	24.72305844143511	24.465030736991384
43	25.277761620602888	24.078952002111382	25.078296350549234	25.564990026736496
44	25.087228787517873	24.525355898992665	24.63381319388334	25.753602119606118
45	25.116737144867223	24.82238662519354	24.74301209333907	25.317864136600164
46	25.01630216296465	24.544766056047788	24.912989183594007	25.525942597393552
47	25.199377818542544	24.49457648807769	25.137135931453997	25.16890976192577
48	25.98833751640618	25.04672076237838	24.20719129773125	24.757750423484193
49	25.112245136175993	24.983411025525058	24.47567168035529	25.428672157943655
50	25.173033519358544	24.961697588214253	24.56138198900309	25.30388690342411
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	31.5
2	43.0
3	130.5
4	218.0
5	283.0
6	348.0
7	323.5
8	299.0
9	396.5
10	494.0
11	779.0
12	1064.0
13	1950.0
14	2836.0
15	3613.5
16	4391.0
17	4675.5
18	4960.0
19	4948.0
20	4936.0
21	5896.0
22	6856.0
23	8198.5
24	9541.0
25	11933.5
26	14326.0
27	17692.5
28	21059.0
29	24145.0
30	27231.0
31	30825.0
32	34419.0
33	39496.0
34	44573.0
35	52673.0
36	60773.0
37	73013.5
38	85254.0
39	95327.0
40	105400.0
41	117228.5
42	129057.0
43	133330.0
44	137603.0
45	151899.5
46	166196.0
47	177886.0
48	189576.0
49	196143.0
50	202710.0
51	198929.0
52	195148.0
53	192865.5
54	190583.0
55	189470.0
56	188357.0
57	182856.0
58	177355.0
59	162020.0
60	146685.0
61	129432.0
62	112179.0
63	97275.0
64	82371.0
65	69637.5
66	56904.0
67	48497.5
68	40091.0
69	36316.5
70	32542.0
71	25013.5
72	17485.0
73	14951.0
74	12417.0
75	9827.0
76	7237.0
77	6198.5
78	5160.0
79	4322.5
80	3485.0
81	2843.5
82	2202.0
83	1797.5
84	1393.0
85	1038.5
86	684.0
87	497.0
88	310.0
89	235.5
90	161.0
91	146.5
92	132.0
93	95.5
94	59.0
95	63.0
96	67.0
97	60.0
98	53.0
99	49.5
100	46.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00542082840545739
2	3.9568090550783867E-4
3	2.7697663385548704E-4
4	0.0
5	0.0
6	1.187042716523516E-4
7	3.956809055078386E-5
8	0.0
9	0.0010683384448711643
10	6.726575393633257E-4
11	0.0
12	1.5827236220313545E-4
13	5.935213582617579E-4
14	3.165447244062709E-4
15	0.004748170866094063
16	5.935213582617579E-4
17	0.0
18	1.5827236220313545E-4
19	3.956809055078386E-5
20	2.374085433047032E-4
21	0.0
22	1.187042716523516E-4
23	8.70497992117245E-4
24	2.7697663385548704E-4
25	0.0015035874409297868
26	0.005064715590500334
27	0.004906443228297199
28	0.003798536692875251
29	0.0018597002558868416
30	0.0030467429724103574
31	0.006647439212531689
32	0.0031654472440627093
33	0.003719400511773683
34	0.0024532216141485994
35	0.002651062066902519
36	0.007359664842445798
37	0.0032445834251642767
38	0.0064891668503285534
39	0.00451076232278936
40	0.002097108799191545
41	0.002690630157453303
42	0.0016222917125821384
43	0.0012661788976250836
44	0.0010683384448711643
45	0.0011870427165235158
46	6.726575393633257E-4
47	0.0020575407086407607
48	0.0015827236220313547
49	3.165447244062709E-4
50	0.0013057469881758675
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2527289.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.13579412646868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.30160708297318	40.82316466649532
2	11.982529839477364	12.494374176505195
3	3.8324200833721602	5.994187934185047
4	1.7919880599998907	3.7370688229297726
5	0.9843448933776455	2.5659801355288847
6	0.629043133018092	1.967739796782006
7	0.4410392019788349	1.6095750325249403
8	0.302433628532199	1.2614093915260507
9	0.24618983653763343	1.1551772370379532
>10	1.3317303584633893	12.429578674602716
>50	0.08276988983962547	3.000476862232249
>100	0.06138026665044377	6.724509649973935
>500	0.008705516700506252	3.158689545741185
>1k	0.0038182090791681854	3.078068073934678
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2548	0.1008194947233973	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.187042716523516E-4
2	0.0	0.0	0.0	0.0	1.187042716523516E-4
3	0.0	0.0	0.0	0.0	1.187042716523516E-4
4	0.0	0.0	0.0	0.0	1.187042716523516E-4
5	0.0	0.0	0.0	0.0	1.187042716523516E-4
6	0.0	0.0	0.0	0.0	1.5827236220313545E-4
7	0.0	0.0	0.0	0.0	1.5827236220313545E-4
8	0.0	0.0	0.0	0.0	1.5827236220313545E-4
9	0.0	0.0	0.0	0.0	1.5827236220313545E-4
10	0.0	0.0	0.0	0.0	1.5827236220313545E-4
11	0.0	0.0	0.0	0.0	1.5827236220313545E-4
12	0.0	0.0	0.0	0.0	3.956809055078386E-4
13	0.0	0.0	0.0	0.0	3.956809055078386E-4
14	0.0	0.0	0.0	0.0	3.956809055078386E-4
15	0.0	0.0	0.0	0.0	5.143851771601902E-4
16	0.0	0.0	0.0	0.0	5.143851771601902E-4
17	0.0	0.0	0.0	0.0	5.143851771601902E-4
18	0.0	0.0	0.0	0.0	5.539532677109741E-4
19	0.0	0.0	0.0	0.0	5.539532677109741E-4
20	0.0	0.0	0.0	0.0	5.93521358261758E-4
21	0.0	0.0	0.0	0.0	5.93521358261758E-4
22	0.0	0.0	0.0	0.0	5.93521358261758E-4
23	0.0	0.0	0.0	3.956809055078386E-5	5.93521358261758E-4
24	0.0	0.0	0.0	3.956809055078386E-5	5.93521358261758E-4
25	0.0	0.0	0.0	3.956809055078386E-5	5.93521358261758E-4
26	0.0	0.0	0.0	1.187042716523516E-4	6.726575393633257E-4
27	0.0	0.0	0.0	2.374085433047032E-4	6.726575393633257E-4
28	0.0	0.0	0.0	9.496341732188128E-4	7.122256299141095E-4
29	0.0	0.0	0.0	0.003165447244062709	7.122256299141095E-4
30	0.0	0.0	0.0	0.007122256299141095	7.122256299141095E-4
31	0.0	0.0	0.0	0.017647368385649603	7.122256299141095E-4
32	0.0	0.0	0.0	0.027895503838302624	7.122256299141095E-4
33	0.0	0.0	0.0	0.03940981818858073	7.122256299141095E-4
34	0.0	0.0	0.0	0.054485260688429384	7.517937204648934E-4
35	0.0	0.0	0.0	0.07292399088509466	7.517937204648934E-4
36	0.0	0.0	0.0	0.09935547537301828	7.517937204648934E-4
37	0.0	0.0	0.0	0.13666818476240747	7.517937204648934E-4
38	0.0	0.0	0.0	0.1940023479704933	7.913618110156772E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2985	0.0	26.900167	1
GGTATCA	1335	0.0	21.257633	1
AACGCAG	4580	0.0	17.194845	6
TCAACGC	4595	0.0	17.138712	4
CAACGCA	4640	0.0	17.019905	5
TATCAAC	4745	0.0	16.736332	2
CGAGCCG	790	0.0	16.707577	15
ATCAACG	4740	0.0	16.707247	3
ACCGTCG	600	0.0	16.499384	8
TAACGGC	205	1.05501385E-10	16.099508	36
AGCGAAA	840	0.0	15.974647	17
TACCGTC	690	0.0	15.941433	7
GTATAGG	320	0.0	15.81191	1
ATACCGT	710	0.0	15.80129	6
GGCGTTA	600	0.0	15.766078	42
CTAGCGG	740	0.0	15.757415	29
ATAACGC	865	0.0	15.512952	3
TCTAGCG	770	0.0	15.429522	28
TAACGCC	870	0.0	15.423797	4
TAATACT	615	0.0	15.380627	4
>>END_MODULE