FastQCFastQC Report
Thu 2 Feb 2017
SRR4064015_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064015_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073716
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43550.2100094709208011No Hit
TATCAACGCAGAGTACTTTTTTTTT27750.13381774553506845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG400.00527784214.25083359
GCGTTAT602.5787867E-514.24431
GTATTAG2300.014.037861
GCATCGC3200.013.06452514
CGCATCG3250.012.8622913
ATCGCCA3400.012.296617516
ACGGTAT2550.012.2948369
CGGACAT1405.2750693E-1112.2126415
GTATCAA26500.011.8254571
CCGCATC3650.011.7127312
TTAGAAC1406.85759E-1011.5313773
CGCCAGT3550.011.51049318
TTAGTAC752.0817069E-411.3957153
TCCAACG1354.7093636E-911.26263618
AACGGAC1104.956037E-711.22982715
AACGCCG3650.011.1902735
CGAACGA1451.2132659E-911.14019616
CGTCGTA2150.011.04715610
TAAGGCC1553.1468517E-1011.0299744
GCGCCAC951.3598088E-511.00064313
Produced by FastQC (version 0.11.5)