##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064015_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2073716
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248340659955364	32.0	32.0	32.0	32.0	32.0
2	30.838555520620954	32.0	32.0	32.0	32.0	32.0
3	30.858735718873753	32.0	32.0	32.0	32.0	32.0
4	30.889495957980746	32.0	32.0	32.0	32.0	32.0
5	30.786352133078974	32.0	32.0	32.0	32.0	32.0
6	34.44338279687286	36.0	36.0	36.0	32.0	36.0
7	34.38873741630966	36.0	36.0	36.0	32.0	36.0
8	34.346944808257255	36.0	36.0	36.0	32.0	36.0
9	34.500744074887784	36.0	36.0	36.0	32.0	36.0
10	34.1795998101958	36.0	36.0	36.0	32.0	36.0
11	34.46529611576513	36.0	36.0	36.0	32.0	36.0
12	34.28306431546075	36.0	36.0	36.0	32.0	36.0
13	34.36690607585609	36.0	36.0	36.0	32.0	36.0
14	34.26770396717776	36.0	36.0	36.0	32.0	36.0
15	34.20181452040684	36.0	36.0	36.0	32.0	36.0
16	34.2002699501764	36.0	36.0	36.0	32.0	36.0
17	34.136390904058224	36.0	36.0	36.0	32.0	36.0
18	34.12212810240168	36.0	36.0	36.0	32.0	36.0
19	34.140899718187065	36.0	36.0	36.0	32.0	36.0
20	34.12527655667411	36.0	36.0	36.0	32.0	36.0
21	34.10495361949274	36.0	36.0	36.0	32.0	36.0
22	34.07964542878581	36.0	36.0	36.0	32.0	36.0
23	34.03466627059829	36.0	36.0	36.0	32.0	36.0
24	34.00877458629822	36.0	36.0	36.0	32.0	36.0
25	33.61271938876876	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	25.0
5	15.0
6	102.0
7	32.0
8	110.0
9	148.0
10	179.0
11	51.0
12	115.0
13	117.0
14	189.0
15	326.0
16	561.0
17	787.0
18	953.0
19	1349.0
20	2000.0
21	3036.0
22	4589.0
23	7059.0
24	10541.0
25	15706.0
26	22460.0
27	30086.0
28	41370.0
29	57025.0
30	74938.0
31	102939.0
32	145137.0
33	205060.0
34	433159.0
35	913548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.69932216815498	17.53076438071081	11.534622681968013	26.235290769166202
2	16.086474693579937	19.83450230128509	38.04518080423452	26.03384220090045
3	18.673162037380852	24.23558674859501	28.81280736390633	28.278443850117807
4	12.397891448464568	15.766346410268653	36.43337282888972	35.40238931237706
5	14.152690037863133	36.930322866274324	34.21508631935727	14.701900776505278
6	33.56033403828949	36.008999645512354	17.223903560068777	13.20676275612938
7	29.464904140194363	30.78694369574906	21.364620332658703	18.383531831397875
8	27.618644239498842	33.5738426464161	19.679521380517347	19.12799173356772
9	27.380898965849138	14.105171236759517	18.726790706874798	39.78713909051655
10	15.42673020869571	27.15611141696248	32.36368531545124	25.053473058890567
11	36.45430052533456	21.15243579994828	23.286679816113605	19.10658385860356
12	24.521331624538703	23.68288992171456	29.597291277426923	22.198487176319812
13	29.381299532517264	19.958395580724734	25.42038703401313	25.23991785274487
14	23.19800920025884	19.971162935686497	25.705666029542552	31.125161834512117
15	24.669731273513506	27.73765138271606	22.53161551938524	25.061001824385194
16	25.026069677093087	25.90990531917922	24.459316586008704	24.604708417718985
17	23.52607097017014	26.22200133177638	25.667335771706508	24.584591926346977
18	24.146612776738856	25.385003317850035	26.74558725945587	23.72279664595524
19	25.398433243460143	24.98307337348752	25.603338321641132	24.0151550614112
20	25.47115313617075	24.901150863111138	25.330715695259443	24.296980305458675
21	26.164010912247903	24.52258915425253	24.746605847887743	24.566794085611825
22	25.479401018378788	24.91176897829611	25.055052393277023	24.55377761004808
23	24.18286616644844	24.86137983589998	25.72348166941679	25.23227232823479
24	24.696658136144436	25.191888491612886	25.557158279231356	24.554295093011326
25	24.52826910631373	25.025976025323065	25.70137681379738	24.74437805456582
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	134.0
1	134.0
2	379.0
3	624.0
4	624.0
5	624.0
6	1719.0
7	2814.0
8	2814.0
9	2814.0
10	3290.0
11	3766.0
12	3766.0
13	3766.0
14	5022.5
15	6279.0
16	6279.0
17	6279.0
18	10608.0
19	14937.0
20	14937.0
21	14937.0
22	24127.5
23	33318.0
24	33318.0
25	33318.0
26	49126.5
27	64935.0
28	64935.0
29	64935.0
30	82864.5
31	100794.0
32	100794.0
33	100794.0
34	120136.5
35	139479.0
36	139479.0
37	139479.0
38	159069.5
39	178660.0
40	178660.0
41	178660.0
42	201710.5
43	224761.0
44	224761.0
45	224761.0
46	247930.5
47	271100.0
48	271100.0
49	271100.0
50	273988.5
51	276877.0
52	276877.0
53	276877.0
54	258323.5
55	239770.0
56	239770.0
57	239770.0
58	217774.5
59	195779.0
60	195779.0
61	195779.0
62	169447.5
63	143116.0
64	143116.0
65	143116.0
66	116166.0
67	89216.0
68	89216.0
69	89216.0
70	66359.0
71	43502.0
72	43502.0
73	43502.0
74	32661.0
75	21820.0
76	21820.0
77	21820.0
78	17112.0
79	12404.0
80	12404.0
81	12404.0
82	8522.0
83	4640.0
84	4640.0
85	4640.0
86	3531.5
87	2423.0
88	2423.0
89	2423.0
90	1732.5
91	1042.0
92	1042.0
93	1042.0
94	761.5
95	481.0
96	481.0
97	481.0
98	763.0
99	1045.0
100	1045.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03775830441584094
2	0.004436480212333801
3	0.001639568774123361
4	0.0031344697152358375
5	0.009403409145707512
6	0.014515005912092108
7	0.02314685328174157
8	0.03635984869673571
9	0.0482708336146319
10	0.05675801315127047
11	0.0545879956561072
12	0.06268939430471675
13	0.06461829874486187
14	0.06669187101801789
15	0.059651369811488164
16	0.06678831624002515
17	0.06249650386070224
18	0.07672217410677258
19	0.07411815311257665
20	0.07807240721487416
21	0.07469682444462018
22	0.0771079549948016
23	0.0813515447631209
24	0.07469682444462018
25	0.0777830715488524
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2073716.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.88979129271421
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5367179124465	39.195845274134754
2	13.954904207223498	14.482341336452937
3	4.489597331718354	6.98892805593576
4	1.9627181046093876	4.07380131258451
5	1.069777968313302	2.7755277752660534
6	0.6377633711702368	1.985604493449685
7	0.43338637571985256	1.5741830009646278
8	0.3036686895611663	1.2605843938768753
9	0.22740584293386565	1.0620037555723831
>10	1.21689609642886	11.399089863205141
>50	0.08813992190130623	3.1824559264002685
>100	0.06905506006955814	7.436010385060501
>500	0.00708086879175514	2.4358330675543525
>1k	0.0028882491124264356	2.1477913595421083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4355	0.2100094709208011	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2775	0.13381774553506845	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9289044401451307E-4
2	0.0	0.0	0.0	0.0	1.9289044401451307E-4
3	0.0	0.0	0.0	0.0	1.9289044401451307E-4
4	0.0	0.0	0.0	0.0	1.9289044401451307E-4
5	0.0	0.0	0.0	0.0	1.9289044401451307E-4
6	0.0	0.0	0.0	0.0	1.9289044401451307E-4
7	0.0	0.0	0.0	9.644522200725653E-5	2.8933566602176963E-4
8	0.0	0.0	0.0	9.644522200725653E-5	2.8933566602176963E-4
9	0.0	0.0	0.0	9.644522200725653E-5	2.8933566602176963E-4
10	0.0	0.0	0.0	2.4111305501814135E-4	3.375582770253979E-4
11	0.0	0.0	0.0	2.4111305501814135E-4	3.8578088802902614E-4
12	0.0	0.0	0.0	2.4111305501814135E-4	6.268939430471675E-4
13	0.0	0.0	0.0	2.4111305501814135E-4	7.715617760580523E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	40	0.005277842	14.2508335	9
GCGTTAT	60	2.5787867E-5	14.2443	1
GTATTAG	230	0.0	14.03786	1
GCATCGC	320	0.0	13.064525	14
CGCATCG	325	0.0	12.86229	13
ATCGCCA	340	0.0	12.2966175	16
ACGGTAT	255	0.0	12.294836	9
CGGACAT	140	5.2750693E-11	12.212641	5
GTATCAA	2650	0.0	11.825457	1
CCGCATC	365	0.0	11.71273	12
TTAGAAC	140	6.85759E-10	11.531377	3
CGCCAGT	355	0.0	11.510493	18
TTAGTAC	75	2.0817069E-4	11.395715	3
TCCAACG	135	4.7093636E-9	11.262636	18
AACGGAC	110	4.956037E-7	11.229827	15
AACGCCG	365	0.0	11.190273	5
CGAACGA	145	1.2132659E-9	11.140196	16
CGTCGTA	215	0.0	11.047156	10
TAAGGCC	155	3.1468517E-10	11.029974	4
GCGCCAC	95	1.3598088E-5	11.000643	13
>>END_MODULE