##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064015_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2073716
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.325958327948474	32.0	32.0	32.0	32.0	32.0
2	31.375239425263633	32.0	32.0	32.0	32.0	32.0
3	31.463310308644	32.0	32.0	32.0	32.0	32.0
4	31.545945539312036	32.0	32.0	32.0	32.0	32.0
5	31.49415541954636	32.0	32.0	32.0	32.0	32.0
6	35.06890914667196	36.0	36.0	36.0	36.0	36.0
7	35.0943861165174	36.0	36.0	36.0	36.0	36.0
8	35.03568858995156	36.0	36.0	36.0	36.0	36.0
9	35.157051881742724	36.0	36.0	36.0	36.0	36.0
10	35.001904310908536	36.0	36.0	36.0	36.0	36.0
11	35.15245771359241	36.0	36.0	36.0	36.0	36.0
12	35.05821867603857	36.0	36.0	36.0	36.0	36.0
13	35.10869665855884	36.0	36.0	36.0	36.0	36.0
14	35.05101180682408	36.0	36.0	36.0	36.0	36.0
15	35.02334553043907	36.0	36.0	36.0	36.0	36.0
16	35.033416822747185	36.0	36.0	36.0	36.0	36.0
17	35.000963487767855	36.0	36.0	36.0	36.0	36.0
18	35.004191027122324	36.0	36.0	36.0	36.0	36.0
19	34.991174779960225	36.0	36.0	36.0	36.0	36.0
20	34.98665294572642	36.0	36.0	36.0	36.0	36.0
21	34.98960658065039	36.0	36.0	36.0	36.0	36.0
22	34.96752641152405	36.0	36.0	36.0	36.0	36.0
23	34.91799793221444	36.0	36.0	36.0	32.0	36.0
24	34.89378053696842	36.0	36.0	36.0	32.0	36.0
25	34.86873901730034	36.0	36.0	36.0	32.0	36.0
26	34.810166869523115	36.0	36.0	36.0	32.0	36.0
27	34.799944158216455	36.0	36.0	36.0	32.0	36.0
28	34.77179758462586	36.0	36.0	36.0	32.0	36.0
29	34.74945604894788	36.0	36.0	36.0	32.0	36.0
30	34.733896541281446	36.0	36.0	36.0	32.0	36.0
31	34.722594125714416	36.0	36.0	36.0	32.0	36.0
32	34.6858745363396	36.0	36.0	36.0	32.0	36.0
33	34.66351226493888	36.0	36.0	36.0	32.0	36.0
34	34.65687442253423	36.0	36.0	36.0	32.0	36.0
35	34.62481265515625	36.0	36.0	36.0	32.0	36.0
36	34.5980519994059	36.0	36.0	36.0	32.0	36.0
37	34.58571135102396	36.0	36.0	36.0	32.0	36.0
38	34.549270006114625	36.0	36.0	36.0	32.0	36.0
39	34.53436150369674	36.0	36.0	36.0	32.0	36.0
40	34.522834852988545	36.0	36.0	36.0	32.0	36.0
41	34.50247574884892	36.0	36.0	36.0	32.0	36.0
42	34.45134386772345	36.0	36.0	36.0	32.0	36.0
43	34.450783520983585	36.0	36.0	36.0	32.0	36.0
44	34.3840545185551	36.0	36.0	36.0	32.0	36.0
45	34.35205351166698	36.0	36.0	36.0	32.0	36.0
46	34.32650324345282	36.0	36.0	36.0	32.0	36.0
47	34.30296289366529	36.0	36.0	36.0	32.0	36.0
48	34.25454883889597	36.0	36.0	36.0	32.0	36.0
49	34.2342596575423	36.0	36.0	36.0	32.0	36.0
50	33.6370959186311	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	6.0
19	5.0
20	11.0
21	60.0
22	183.0
23	573.0
24	1492.0
25	3645.0
26	7370.0
27	13465.0
28	22854.0
29	35742.0
30	51792.0
31	74851.0
32	109482.0
33	177526.0
34	368508.0
35	1206148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17250598979131	18.354321716417623	12.058916011744234	26.414256282046832
2	15.489578069001169	20.609016523091046	37.70340415025481	26.198001257652976
3	17.792853288479723	24.96034882425644	29.197635733846937	28.049162153416894
4	12.0972688642032	16.51971629673494	36.621697474485416	34.76131736457644
5	13.894911357196454	37.35328270602146	33.89851840849952	14.85328752828256
6	33.56216602779652	36.403735714633605	17.015180017678436	13.018918239891441
7	29.424969197792368	30.916205939581857	21.39618992966729	18.262634932958484
8	27.588892398425045	33.89043335270276	19.481992462802268	19.038681786069926
9	27.447136734887756	13.972133853563125	18.529352620606947	40.05137679094218
10	15.35171982778575	27.339349644306594	32.40503911855932	24.903891409348333
11	36.75061423580386	21.15126717027171	23.201645356280878	18.89647323764355
12	24.71634158891146	23.61107561379152	29.55188023006121	22.12070256723581
13	29.554478793040097	19.925534284995074	25.371822235167375	25.148164686797454
14	23.29709713368655	19.945614355726864	25.614972978368712	31.142315532217875
15	24.701065530486613	27.88632412477726	22.404737620050007	25.007872724686113
16	25.08363058740813	25.982650360249277	24.430547672448753	24.503171379893843
17	23.68569273709611	26.287833049462893	25.62544726471706	24.401026948723935
18	24.307098350924214	25.38724130265269	26.744665471062206	23.560994875360887
19	25.54174512891116	25.03564858237511	25.60737613413608	23.815230154577655
20	25.723091226748696	24.764418785543334	25.31801836426756	24.194471623440407
21	26.289810176514045	24.489804775581614	24.791774765686334	24.428610282218006
22	25.571305603711213	24.85953952206547	25.153504700501273	24.41565017372204
23	24.276653858617657	24.790808295912225	25.788881301791584	25.14365654367853
24	24.83162994175645	25.19392816532334	25.55468325789673	24.41975863502348
25	24.693045844563365	24.919261930837	25.726763181029288	24.660929043570345
26	24.307401175634872	25.539170226894925	26.025666436632278	24.127762160837925
27	24.815029509436364	25.350964084233308	25.227602197944226	24.606404208386103
28	24.270444270299596	25.14943637350051	25.82872988124661	24.751389474953285
29	24.511467175397293	25.180671065334785	25.505700554286193	24.80216120498173
30	24.360626976904783	25.259484199952453	25.853996343615286	24.525892479527478
31	24.995370272131545	24.92659952275222	25.21566815653882	24.86236204857742
32	24.587044384078503	25.27346720684166	25.0906011576694	25.048887251410434
33	24.248423158293736	25.139575628361595	25.63928458960642	24.97271662373825
34	24.832891520423136	25.067839219001442	25.80103807146133	24.298231189114087
35	25.196923679355105	25.021773243842006	25.616136987701854	24.165166089101035
36	24.402651122212916	25.43938207999182	25.447146590584108	24.710820207211157
37	25.029561772047394	25.203653121298753	25.151666840275766	24.61511826637809
38	24.619312474108426	25.36142105981478	25.43491816695128	24.584348299125512
39	25.159552356417347	24.964434075001424	24.98058944432281	24.89542412425842
40	25.440984000254623	25.099992380614356	25.461430959203497	23.99759265992753
41	24.556168217718934	25.254937438694935	25.820607760437415	24.368286583148716
42	25.316793261804786	25.679772731547096	25.225071865305537	23.778362141342576
43	24.62706576199914	25.0261610944741	25.609372664187998	24.737400479338763
44	24.379654759355812	25.452045731172156	25.244733527384117	24.92356598208792
45	24.48979690251745	25.55649751842615	25.33954064360819	24.614164935448215
46	24.518840114250608	25.122450977413656	25.32146847619723	25.037240432138503
47	24.947243344174005	25.088393921507357	25.506155264670593	24.458207469648045
48	25.230691033876802	25.64073877070553	24.70606333436372	24.422506861053954
49	24.727697789514142	25.531282866865958	24.940649763925183	24.80036957969472
50	24.58377316945791	25.943041603634875	24.82696296310585	24.64622226380137
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	22.5
2	31.0
3	106.0
4	181.0
5	224.0
6	267.0
7	280.5
8	294.0
9	386.0
10	478.0
11	718.0
12	958.0
13	1763.0
14	2568.0
15	3510.5
16	4453.0
17	4847.0
18	5241.0
19	5324.5
20	5408.0
21	6334.0
22	7260.0
23	8414.0
24	9568.0
25	12021.0
26	14474.0
27	18258.5
28	22043.0
29	26010.0
30	29977.0
31	34446.5
32	38916.0
33	45450.5
34	51985.0
35	60005.0
36	68025.0
37	77360.0
38	86695.0
39	92053.0
40	97411.0
41	104194.0
42	110977.0
43	112256.0
44	113535.0
45	123792.0
46	134049.0
47	142032.5
48	150016.0
49	154922.0
50	159828.0
51	157267.5
52	154707.0
53	152118.5
54	149530.0
55	147705.0
56	145880.0
57	141524.5
58	137169.0
59	123977.5
60	110786.0
61	95989.0
62	81192.0
63	69963.0
64	58734.0
65	49277.5
66	39821.0
67	33637.0
68	27453.0
69	24110.0
70	20767.0
71	16112.0
72	11457.0
73	9753.0
74	8049.0
75	6322.5
76	4596.0
77	4025.0
78	3454.0
79	2860.0
80	2266.0
81	1845.0
82	1424.0
83	1173.0
84	922.0
85	666.5
86	411.0
87	287.5
88	164.0
89	134.5
90	105.0
91	82.0
92	59.0
93	54.0
94	49.0
95	40.5
96	32.0
97	26.5
98	21.0
99	18.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009065850868682116
2	5.786713320435392E-4
3	3.375582770253979E-4
4	0.0
5	0.0
6	1.446678330108848E-4
7	4.8222611003628274E-5
8	4.8222611003628274E-5
9	8.197843870616805E-4
10	9.644522200725654E-4
11	4.8222611003628274E-5
12	3.375582770253979E-4
13	4.822261100362827E-4
14	3.375582770253979E-4
15	0.004870483711366455
16	6.268939430471675E-4
17	0.0
18	4.340034990326544E-4
19	4.8222611003628274E-5
20	7.23339165054424E-4
21	0.0
22	4.8222611003628274E-5
23	9.644522200725654E-4
24	4.822261100362827E-4
25	0.0016877913851269892
26	0.005449155043409994
27	0.006847610762515215
28	0.004388257601330173
29	0.0027969114382104397
30	0.004002476713301146
31	0.007715617760580523
32	0.003230914937243094
33	0.005256264599395481
34	0.0030380244932285807
35	0.0033755827702539785
36	0.00863184736964946
37	0.004436480212333801
38	0.007956730815598664
39	0.004918706322370083
40	0.0027969114382104397
41	0.0033755827702539785
42	0.002170017495163272
43	0.001253787886094335
44	0.0015431235521161048
45	0.0015431235521161048
46	0.0013984557191052199
47	0.002121794884159644
48	0.002652243605199555
49	4.340034990326544E-4
50	0.0013020104970979632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2073716.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.77292916742883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.24142740123544	41.75966302671536
2	13.660656813160085	14.964683760155426
3	4.439334230263598	7.294660180343107
4	1.8851437004322142	4.130193694967933
5	1.0239319993495997	2.80418774363197
6	0.6172552465251935	2.0285326737688942
7	0.40078111457830456	1.5366368920308455
8	0.28486562055907555	1.2482339565694323
9	0.20633715687527954	1.0171521430324504
>10	1.0974541156785418	10.622862708369055
>50	0.07952659409283161	3.015763337574974
>100	0.05593127967392603	6.285159538474463
>500	0.005582504063541427	2.0466697352553513
>1k	0.0017722235122353683	1.2456006091107976
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.822261100362827E-5
6	0.0	0.0	0.0	0.0	4.822261100362827E-5
7	0.0	0.0	0.0	0.0	4.822261100362827E-5
8	0.0	0.0	0.0	0.0	4.822261100362827E-5
9	0.0	0.0	0.0	0.0	4.822261100362827E-5
10	0.0	0.0	0.0	0.0	4.822261100362827E-5
11	0.0	0.0	0.0	0.0	4.822261100362827E-5
12	0.0	0.0	0.0	0.0	2.4111305501814135E-4
13	0.0	0.0	0.0	0.0	2.4111305501814135E-4
14	0.0	0.0	0.0	0.0	2.4111305501814135E-4
15	0.0	0.0	0.0	0.0	2.8933566602176963E-4
16	0.0	0.0	0.0	0.0	2.8933566602176963E-4
17	0.0	0.0	0.0	4.822261100362827E-5	2.8933566602176963E-4
18	0.0	0.0	0.0	4.822261100362827E-5	3.375582770253979E-4
19	0.0	0.0	0.0	4.822261100362827E-5	3.375582770253979E-4
20	0.0	0.0	0.0	4.822261100362827E-5	5.304487210399109E-4
21	0.0	0.0	0.0	4.822261100362827E-5	6.268939430471675E-4
22	0.0	0.0	0.0	9.644522200725653E-5	6.268939430471675E-4
23	0.0	0.0	0.0	9.644522200725653E-5	6.268939430471675E-4
24	0.0	0.0	0.0	1.9289044401451307E-4	6.268939430471675E-4
25	0.0	0.0	0.0	2.8933566602176963E-4	6.268939430471675E-4
26	0.0	0.0	0.0	2.8933566602176963E-4	6.268939430471675E-4
27	0.0	0.0	0.0	5.786713320435393E-4	6.751165540507958E-4
28	0.0	0.0	0.0	0.0016395687741233612	6.751165540507958E-4
29	0.0	0.0	0.0	0.0038578088802902616	6.751165540507958E-4
30	0.0	0.0	0.0	0.007474504705562382	6.751165540507958E-4
31	0.0	0.0	0.0	0.01533479029915379	6.751165540507958E-4
32	0.0	0.0	0.0	0.024063082890810505	6.751165540507958E-4
33	0.0	0.0	0.0	0.03438272164558696	6.751165540507958E-4
34	0.0	0.0	0.0	0.04450946995634889	9.162296090689371E-4
35	0.0	0.0	0.0	0.05777068798234666	9.162296090689371E-4
36	0.0	0.0	0.0	0.07797596199286691	9.162296090689371E-4
37	0.0	0.0	0.0	0.10816331648113821	9.162296090689371E-4
38	0.0	0.0	0.0	0.15141899855139276	9.644522200725654E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3015	0.0	23.49449	1
CGGATCG	120	1.4319085E-8	20.167927	26
ATACCGT	390	0.0	18.049797	6
AACGCAG	4025	0.0	17.434591	6
CGAGCCG	630	0.0	16.76093	15
CGTCTTA	290	0.0	16.688686	15
TACCGTC	400	0.0	16.499043	7
TCAACGC	4325	0.0	16.174004	4
GGTATCA	1510	0.0	16.171246	1
CGTCGTA	385	0.0	16.000229	10
ATTTCGT	345	0.0	15.940719	42
TATCAAC	4515	0.0	15.931477	2
CAACGCA	4415	0.0	15.844677	5
ATCGTTT	445	0.0	15.821214	29
ACCGTCG	405	0.0	15.7521715	8
GTCCTAA	590	0.0	15.660108	1
ATCAACG	4505	0.0	15.527386	3
TAACGGC	185	7.557901E-9	15.46229	36
TTAACGG	185	7.557901E-9	15.46229	35
GTCGGAA	470	0.0	15.447773	39
>>END_MODULE