FastQCFastQC Report
Thu 2 Feb 2017
SRR4064014_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064014_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308140
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39980.30562478022230033No Hit
TATCAACGCAGAGTACTTTTTTTTT28850.22054214380723774No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22590.17268793859984408No Hit
GTCCTAAAGTGTGTATTTCTCATTT15710.12009417952206951No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15490.11841240234225694No Hit
GGTATCAACGCAGAGTACTTTTTTT14370.10985062760866574No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13890.1061812955799838No Hit
GTACATGGGGTGGTATCAACGCAAA13090.10006574219884723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCT759.692831E-713.9303324
CCGTCGT1700.013.4134949
CGTCGTA1650.013.24362510
GAGTAAG802.008719E-613.0566891
CCGACCA1850.012.8394939
CGACCAT1800.012.66781410
TACACCG604.0947273E-412.6653925
AGGCCCG2000.012.35111810
AAAGGCG853.9463957E-612.292885
GCGCAAG2550.012.288651
AGGACCT6750.012.2432115
GTGTAGG1251.4188117E-912.1545911
GTATTAG2150.011.9249251
ATTACCG1603.6379788E-1211.87698515
TAGGACA3200.011.8724414
GCAAGAC3100.011.6413173
GCGTTAT1157.1124305E-811.5600731
CAAGACG2550.011.5465334
AATGTCC2250.011.4014698
AAGACGG2750.011.3988525
Produced by FastQC (version 0.11.5)