##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064014_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1308140
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.145445441619398	32.0	32.0	32.0	32.0	32.0
2	30.690479612273915	32.0	32.0	32.0	32.0	32.0
3	30.70587093124589	32.0	32.0	32.0	32.0	32.0
4	30.738164110874983	32.0	32.0	32.0	32.0	32.0
5	30.627497821334106	32.0	32.0	32.0	32.0	32.0
6	34.276886265996	36.0	36.0	36.0	32.0	36.0
7	34.1890157016833	36.0	36.0	36.0	32.0	36.0
8	34.12890210527925	36.0	36.0	36.0	32.0	36.0
9	34.29924090693657	36.0	36.0	36.0	32.0	36.0
10	33.9455822771263	36.0	36.0	36.0	32.0	36.0
11	34.306033757854664	36.0	36.0	36.0	32.0	36.0
12	34.07697876374088	36.0	36.0	36.0	32.0	36.0
13	34.177817358998276	36.0	36.0	36.0	32.0	36.0
14	34.06154998700445	36.0	36.0	36.0	32.0	36.0
15	33.99543397495681	36.0	36.0	36.0	32.0	36.0
16	34.00314798110294	36.0	36.0	36.0	32.0	36.0
17	33.9148317458376	36.0	36.0	36.0	32.0	36.0
18	33.91976776186035	36.0	36.0	36.0	32.0	36.0
19	33.92163529897412	36.0	36.0	36.0	32.0	36.0
20	33.889722812543	36.0	36.0	36.0	32.0	36.0
21	33.878809607534365	36.0	36.0	36.0	32.0	36.0
22	33.861121133823595	36.0	36.0	36.0	32.0	36.0
23	33.79915911141009	36.0	36.0	36.0	32.0	36.0
24	33.78029950922684	36.0	36.0	36.0	32.0	36.0
25	33.381336095524944	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	14.0
5	13.0
6	44.0
7	20.0
8	83.0
9	74.0
10	116.0
11	38.0
12	71.0
13	58.0
14	148.0
15	215.0
16	387.0
17	482.0
18	647.0
19	897.0
20	1446.0
21	2192.0
22	3538.0
23	5548.0
24	8268.0
25	12181.0
26	17092.0
27	22512.0
28	30335.0
29	40498.0
30	53023.0
31	71143.0
32	98551.0
33	135376.0
34	275923.0
35	527201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.626716946040425	17.854709332248415	11.742109944196624	25.776463777514536
2	15.7208264752179	20.373973385033267	39.13708917244685	24.768110967301986
3	18.434124906735818	24.939760509803442	29.161188032828996	27.464926550631752
4	12.04795698152037	16.07925760403153	37.37054588793271	34.502239526515396
5	13.663392526765483	37.61448531682808	34.41967603139994	14.3024461250065
6	32.48714043682759	36.965968794534454	17.574863758968053	12.972027009669901
7	28.878942213366383	31.301520113345205	21.658002405450723	18.161535267837685
8	27.106889574408537	34.2119591400382	20.042349616039324	18.638801669513935
9	27.54754211200551	14.055715951893847	19.086441941836675	39.31029999426397
10	15.423900065701968	27.28260794741395	32.695103864943064	24.598388121941014
11	36.42871729448558	21.17298921652085	23.83493835007186	18.563355138921715
12	24.58766600831935	23.951399587245298	30.153735062884664	21.307199341550692
13	29.434217138783435	20.24807643618067	25.67635479397463	24.641351631061262
14	22.822624813739377	20.657259039185867	26.207443081507954	30.3126730655668
15	24.129253532244544	29.12594922379347	22.827034750310542	23.917762493651445
16	24.26723353216462	26.22698893755555	25.721751449163392	23.784026081116433
17	22.777357497615366	26.903939253240765	26.517655463926832	23.801047785217033
18	23.44565105132903	25.62657068213027	28.261300304860555	22.666477961680137
19	25.00589039880295	25.0385553376193	26.306674051339797	23.648880212237955
20	25.24794893215946	25.05829592295758	26.106627986327236	23.587127158555727
21	25.85355106312338	24.778111148681212	25.19266577212424	24.175672016071164
22	25.18543962349024	25.54645017763647	25.78023874586509	23.48787145300819
23	23.392760008599183	25.507133725702914	26.508280538382266	24.591825727315637
24	24.22212141125494	25.594621080389267	26.606113785661773	23.577143722694018
25	23.968313468688322	25.55401704432442	26.605670221392618	23.87199926559464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	298.0
1	298.0
2	534.5
3	771.0
4	771.0
5	771.0
6	1973.5
7	3176.0
8	3176.0
9	3176.0
10	3272.0
11	3368.0
12	3368.0
13	3368.0
14	3839.0
15	4310.0
16	4310.0
17	4310.0
18	7693.5
19	11077.0
20	11077.0
21	11077.0
22	17816.0
23	24555.0
24	24555.0
25	24555.0
26	37226.5
27	49898.0
28	49898.0
29	49898.0
30	63672.0
31	77446.0
32	77446.0
33	77446.0
34	89330.0
35	101214.0
36	101214.0
37	101214.0
38	111354.5
39	121495.0
40	121495.0
41	121495.0
42	132754.0
43	144013.0
44	144013.0
45	144013.0
46	155845.0
47	167677.0
48	167677.0
49	167677.0
50	166744.5
51	165812.0
52	165812.0
53	165812.0
54	150078.5
55	134345.0
56	134345.0
57	134345.0
58	121475.5
59	108606.0
60	108606.0
61	108606.0
62	94558.5
63	80511.0
64	80511.0
65	80511.0
66	66625.0
67	52739.0
68	52739.0
69	52739.0
70	39709.0
71	26679.0
72	26679.0
73	26679.0
74	20585.0
75	14491.0
76	14491.0
77	14491.0
78	11609.0
79	8727.0
80	8727.0
81	8727.0
82	6010.0
83	3293.0
84	3293.0
85	3293.0
86	2525.0
87	1757.0
88	1757.0
89	1757.0
90	1283.5
91	810.0
92	810.0
93	810.0
94	565.5
95	321.0
96	321.0
97	321.0
98	536.0
99	751.0
100	751.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03921598605653829
2	0.006039108963872368
3	0.0021404436833978016
4	0.0038986652804745667
5	0.008561774733591206
6	0.01253688443132998
7	0.021175103582185394
8	0.034705765437950065
9	0.04701331661748742
10	0.05511642484749339
11	0.05175287048786827
12	0.06314308866023514
13	0.06123197822862996
14	0.06360175516382038
15	0.05779197945174064
16	0.0637546439983488
17	0.06199642240127203
18	0.07529775100524409
19	0.07094041922118427
20	0.07766752794043451
21	0.07292797407005366
22	0.07460975124986623
23	0.07942574953751128
24	0.07361597382543153
25	0.0733866405736389
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1308140.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.723067309364524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.26771862880122	38.930772768597954
2	14.46856123848715	14.967167336158669
3	4.669793138959692	7.246080745416624
4	2.0072685481953743	4.152883449051189
5	1.0183618553050395	2.633639939361595
6	0.5721254905371078	1.7755251153872416
7	0.37336936516953895	1.3518266164143136
8	0.26222039497561245	1.0850274511369404
9	0.1878314399234267	0.8743696388976834
>10	0.9901784940582522	9.315856389378187
>50	0.08688593772752538	3.1938281212281012
>100	0.08413212887573432	9.104278543891976
>500	0.008887183834144033	3.2180899138486
>1k	0.0026661551502432097	2.150653971230994
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3998	0.30562478022230033	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2885	0.22054214380723774	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2259	0.17268793859984408	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1571	0.12009417952206951	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1549	0.11841240234225694	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1437	0.10985062760866574	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1389	0.1061812955799838	No Hit
GTACATGGGGTGGTATCAACGCAAA	1309	0.10006574219884723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.115553381136576E-4
2	0.0	0.0	0.0	0.0	6.115553381136576E-4
3	0.0	0.0	0.0	0.0	6.115553381136576E-4
4	0.0	0.0	0.0	0.0	6.115553381136576E-4
5	0.0	0.0	0.0	0.0	6.115553381136576E-4
6	0.0	0.0	0.0	0.0	6.879997553778648E-4
7	0.0	0.0	0.0	0.0	6.879997553778648E-4
8	0.0	0.0	0.0	0.0	6.879997553778648E-4
9	0.0	0.0	0.0	0.0	6.879997553778648E-4
10	0.0	0.0	0.0	0.0	6.879997553778648E-4
11	7.64444172642072E-5	0.0	0.0	0.0	6.879997553778648E-4
12	7.64444172642072E-5	0.0	0.0	0.0	9.173330071704863E-4
13	7.64444172642072E-5	0.0	0.0	0.0	0.0013759995107557295
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCCT	75	9.692831E-7	13.930332	4
CCGTCGT	170	0.0	13.413494	9
CGTCGTA	165	0.0	13.243625	10
GAGTAAG	80	2.008719E-6	13.056689	1
CCGACCA	185	0.0	12.839493	9
CGACCAT	180	0.0	12.667814	10
TACACCG	60	4.0947273E-4	12.665392	5
AGGCCCG	200	0.0	12.351118	10
AAAGGCG	85	3.9463957E-6	12.29288	5
GCGCAAG	255	0.0	12.28865	1
AGGACCT	675	0.0	12.243211	5
GTGTAGG	125	1.4188117E-9	12.154591	1
GTATTAG	215	0.0	11.924925	1
ATTACCG	160	3.6379788E-12	11.876985	15
TAGGACA	320	0.0	11.872441	4
GCAAGAC	310	0.0	11.641317	3
GCGTTAT	115	7.1124305E-8	11.560073	1
CAAGACG	255	0.0	11.546533	4
AATGTCC	225	0.0	11.401469	8
AAGACGG	275	0.0	11.398852	5
>>END_MODULE