FastQCFastQC Report
Thu 2 Feb 2017
SRR4064014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308140
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13450.10281774122035867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG250.002353224735.198944
CGATTAA450.001397595624.44648433
TTAGGAC6600.021.9993133
ATTGTCG803.6169004E-621.99931343
TAGGACG9600.020.3944154
GTCGTAA1001.1226821E-619.80165130
AATCCCG3450.019.76674319
GACGTGA6100.019.4740567
GTATCAA30700.019.3501471
CGTCGTA2900.018.96564910
GTCCTAC9050.018.9629061
TAACGGC1051.7921429E-618.85799436
GGACGTG10100.018.7313736
AGGACGT10200.018.5477315
GATATAC4650.018.4531521
TTTAGGA7550.018.3570422
ATTGCCG600.007410488618.33416228
GTGCGAA600.007412122618.33346211
ATCCCGT3600.018.3327620
ATACCGT3400.018.1163866