##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064014_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1308140
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.282818352775696	32.0	32.0	32.0	32.0	32.0
2	31.381865855336585	32.0	32.0	32.0	32.0	32.0
3	31.459153454523218	32.0	32.0	32.0	32.0	32.0
4	31.536236947115754	32.0	32.0	32.0	32.0	32.0
5	31.492022260614306	32.0	32.0	32.0	32.0	32.0
6	35.06068922286605	36.0	36.0	36.0	36.0	36.0
7	35.076966532634124	36.0	36.0	36.0	36.0	36.0
8	35.02891586527436	36.0	36.0	36.0	36.0	36.0
9	35.14199932728913	36.0	36.0	36.0	36.0	36.0
10	34.98917852829208	36.0	36.0	36.0	36.0	36.0
11	35.144073264329506	36.0	36.0	36.0	36.0	36.0
12	35.038871221734674	36.0	36.0	36.0	36.0	36.0
13	35.078200345528764	36.0	36.0	36.0	36.0	36.0
14	35.031836806458024	36.0	36.0	36.0	36.0	36.0
15	35.00693733086673	36.0	36.0	36.0	36.0	36.0
16	35.01507407464033	36.0	36.0	36.0	36.0	36.0
17	34.974914764474754	36.0	36.0	36.0	36.0	36.0
18	34.9680982157873	36.0	36.0	36.0	36.0	36.0
19	34.96366520403015	36.0	36.0	36.0	36.0	36.0
20	34.966256669775404	36.0	36.0	36.0	36.0	36.0
21	34.95140504838932	36.0	36.0	36.0	36.0	36.0
22	34.935485498494046	36.0	36.0	36.0	36.0	36.0
23	34.88961196813797	36.0	36.0	36.0	32.0	36.0
24	34.863539070741666	36.0	36.0	36.0	32.0	36.0
25	34.838094546455274	36.0	36.0	36.0	32.0	36.0
26	34.77562646199948	36.0	36.0	36.0	32.0	36.0
27	34.76915926429893	36.0	36.0	36.0	32.0	36.0
28	34.73635620040668	36.0	36.0	36.0	32.0	36.0
29	34.712165364563425	36.0	36.0	36.0	32.0	36.0
30	34.67821410552387	36.0	36.0	36.0	32.0	36.0
31	34.67018591282279	36.0	36.0	36.0	32.0	36.0
32	34.62164523674836	36.0	36.0	36.0	32.0	36.0
33	34.60887596128855	36.0	36.0	36.0	32.0	36.0
34	34.59633372574801	36.0	36.0	36.0	32.0	36.0
35	34.544567859709204	36.0	36.0	36.0	32.0	36.0
36	34.510327640772395	36.0	36.0	36.0	32.0	36.0
37	34.497946702952284	36.0	36.0	36.0	32.0	36.0
38	34.453182381090706	36.0	36.0	36.0	32.0	36.0
39	34.430083171525986	36.0	36.0	36.0	32.0	36.0
40	34.418991086580945	36.0	36.0	36.0	32.0	36.0
41	34.38993838579969	36.0	36.0	36.0	32.0	36.0
42	34.32724326142462	36.0	36.0	36.0	32.0	36.0
43	34.325468986499914	36.0	36.0	36.0	32.0	36.0
44	34.23870763068173	36.0	36.0	36.0	32.0	36.0
45	34.21224486675738	36.0	36.0	36.0	32.0	36.0
46	34.15178803491981	36.0	36.0	36.0	32.0	36.0
47	34.118777042212606	36.0	36.0	36.0	32.0	36.0
48	34.065252190132554	36.0	36.0	36.0	32.0	36.0
49	34.03798523093858	36.0	36.0	36.0	32.0	36.0
50	33.444535753053955	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	0.0
19	4.0
20	9.0
21	33.0
22	163.0
23	401.0
24	1082.0
25	2582.0
26	5225.0
27	9499.0
28	16256.0
29	24690.0
30	35291.0
31	50053.0
32	71569.0
33	114291.0
34	231441.0
35	745549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.753780523233594	18.74841363281907	12.361584685249461	26.136221158697882
2	14.917015572527953	21.170259689925444	38.618049944310116	25.29467479323649
3	17.61882374525565	25.493699044823355	29.724225710649133	27.163251499271862
4	11.675661626431422	16.791551363003958	37.454706682770954	34.078080327793664
5	13.25370373201645	38.063892243949425	34.10300120782179	14.579402816212331
6	32.334738383870814	37.47632130555033	17.350310135475002	12.838630175103848
7	28.683845805141207	31.36720389378177	21.75146792728896	18.197482373788066
8	26.901554879447154	34.42926598070543	19.979742229424986	18.689436910422433
9	27.409536550406116	13.91105590062112	19.070654562828476	39.60875298614429
10	15.22618581667849	27.484389906798114	32.74060336603146	24.54882091049194
11	36.40948216551745	21.23572400507591	23.807849312764688	18.54694451664195
12	24.464162037950125	24.04142376415944	30.193909205297913	21.300504992592526
13	29.353446430646358	20.371735048955156	25.744800234837967	24.530018285560523
14	22.805350514013835	20.591436975971916	26.292908382614648	30.310304127399597
15	24.107019995535484	29.079070891038246	22.883329307112955	23.930579806313318
16	24.24879235731699	26.256930710856057	25.719597562010417	23.77467936981654
17	22.76959864356922	26.84462430980194	26.466453488505426	23.91932355812341
18	23.360685187648397	25.584991797501488	28.27530428746814	22.779018727381974
19	25.177886158973813	25.09081596770988	26.317290198296817	23.414007675019494
20	25.391015585583105	25.008179602670065	26.121217124877305	23.47958768686952
21	25.89776323635085	24.66387389728928	25.363110982004983	24.075251884354888
22	25.156730286307493	25.504934873128924	25.90588614818456	23.432448692379023
23	23.402432023141447	25.333892911173134	26.691480733169282	24.572194332516137
24	24.297244323220216	25.44964743726952	26.60831901270204	23.64478922680822
25	24.001715440261933	25.356179369002362	26.72241592699135	23.919689263744353
26	23.224313997703515	26.376247433272738	27.176500627634574	23.222937941389173
27	24.057201656787	25.899299275117464	26.230167406431132	23.813331661664403
28	23.187418631549896	25.94486783432065	26.697643057489035	24.170070476640422
29	23.659653256255382	25.362029121337375	26.38181869609124	24.596498926316
30	23.25419789847068	25.889709845232957	26.77534888521094	24.080743371085433
31	24.352949540958267	25.376304134930518	25.923606954163102	24.347139369948113
32	23.667987670629678	26.205492563236948	25.847414868633496	24.279104897499877
33	23.28097419043622	25.686788509274272	26.798493365472016	24.233743934817493
34	24.154200863534623	25.799319935907626	26.6955734677153	23.35090573284245
35	24.38724197348525	25.793630771912284	26.56321907618825	23.255908178414224
36	23.348518752680512	26.24366138069329	26.47453887571834	23.93328099090786
37	24.175382293739503	26.009658349457336	25.781463216244127	24.03349614055903
38	23.731861205973217	26.145780057016648	26.129725489836314	23.99263324717382
39	24.560195095101218	25.395847348786006	25.8189101584001	24.22504739771268
40	24.955661424521985	25.72623528800198	26.337878292139226	22.980224995336805
41	23.934503428257344	25.68291849470109	26.906449741189313	23.476128335852255
42	24.640511883557192	26.575746687969666	26.162020013607417	22.62172141486572
43	23.489547579041034	25.978390328701234	26.581164696151266	23.95089739610647
44	23.204846042294317	26.361745376376312	25.899555009903537	24.53385357142584
45	23.422820125890016	26.64292277655211	25.966305998046057	23.96795109951182
46	23.53750597228858	25.76428093645485	25.885064500716677	24.813148590539893
47	24.250272721921494	25.737610550802724	26.130009043588874	23.88210768368691
48	24.24360567962731	26.21078896793399	25.346414762016156	24.199190590422546
49	23.89143619843227	26.342102567473972	25.636135136002885	24.13032609809087
50	23.79567992025221	26.99201298959426	25.266870724793673	23.94543636535986
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	143.0
1	94.0
2	45.0
3	125.0
4	205.0
5	250.0
6	295.0
7	280.0
8	265.0
9	386.5
10	508.0
11	768.5
12	1029.0
13	1792.5
14	2556.0
15	3443.5
16	4331.0
17	4745.0
18	5159.0
19	5258.5
20	5358.0
21	5924.5
22	6491.0
23	7622.5
24	8754.0
25	10160.0
26	11566.0
27	14491.5
28	17417.0
29	20869.0
30	24321.0
31	28231.5
32	32142.0
33	38064.0
34	43986.0
35	50492.0
36	56998.0
37	61078.0
38	65158.0
39	67377.0
40	69596.0
41	71294.5
42	72993.0
43	72688.5
44	72384.0
45	77239.0
46	82094.0
47	85722.5
48	89351.0
49	91196.5
50	93042.0
51	89541.5
52	86041.0
53	83928.0
54	81815.0
55	80900.0
56	79985.0
57	77677.5
58	75370.0
59	68105.5
60	60841.0
61	53581.0
62	46321.0
63	40185.5
64	34050.0
65	28826.0
66	23602.0
67	20510.5
68	17419.0
69	15805.0
70	14191.0
71	10986.0
72	7781.0
73	6626.0
74	5471.0
75	4333.5
76	3196.0
77	2767.5
78	2339.0
79	1935.5
80	1532.0
81	1216.5
82	901.0
83	743.0
84	585.0
85	420.5
86	256.0
87	176.0
88	96.0
89	74.0
90	52.0
91	47.0
92	42.0
93	30.0
94	18.0
95	23.0
96	28.0
97	19.0
98	10.0
99	10.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009173330071704863
2	2.293332517926216E-4
3	3.822220863210359E-4
4	0.0
5	0.0
6	1.528888345284144E-4
7	2.293332517926216E-4
8	0.0
9	0.0011466662589631079
10	9.173330071704864E-4
11	0.0
12	1.528888345284144E-4
13	3.057776690568288E-4
14	3.057776690568288E-4
15	0.0036693320286819457
16	9.937774244346935E-4
17	7.64444172642072E-5
18	1.528888345284144E-4
19	0.0
20	6.115553381136576E-4
21	0.0
22	7.64444172642072E-5
23	8.408885899062792E-4
24	3.057776690568288E-4
25	0.0016053327625483512
26	0.004127998532267189
27	0.005045331539437674
28	0.003592887611417738
29	0.0020639992661335943
30	0.003439998776889324
31	0.00680355313651444
32	0.003363554359625117
33	0.004510220618588225
34	0.0021404436833978016
35	0.0029048878560398736
36	0.006650664301986026
37	0.0037457764459461525
38	0.007720886143684927
39	0.0045866650358524315
40	0.0018346660143409728
41	0.003134221107832495
42	0.0022933325179262157
43	0.0016817771798125585
44	0.0016053327625483512
45	0.0010702218416989008
46	0.0011466662589631079
47	0.002369776935190423
48	0.001987554848869387
49	4.586665035852432E-4
50	0.0012231106762273152
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1308140.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.01214648647863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.38834769290061	42.7867532083481
2	14.031335978361428	15.718504924419566
3	4.489427038402603	7.543873347460936
4	1.880279761547797	4.212740217575052
5	0.9373158443081206	2.625053618774192
6	0.5434434738844567	1.826366127980221
7	0.3362947882061165	1.318561505774821
8	0.23252294994065936	1.041928762683548
9	0.1672732794248675	0.8432401887377414
>10	0.8509077864008622	8.477820702298322
>50	0.06791218691201319	2.6577558323885597
>100	0.06697866618448642	7.6220019208116785
>500	0.0067252952202882105	2.5588572015175823
>1k	0.0012352583057672222	0.7665424412297375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1345	0.10281774122035867	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2933325179262158E-4
2	0.0	0.0	0.0	0.0	2.2933325179262158E-4
3	0.0	0.0	0.0	0.0	2.2933325179262158E-4
4	0.0	0.0	0.0	0.0	2.2933325179262158E-4
5	0.0	0.0	0.0	0.0	3.057776690568288E-4
6	0.0	0.0	0.0	0.0	4.5866650358524316E-4
7	0.0	0.0	0.0	0.0	4.5866650358524316E-4
8	0.0	0.0	0.0	0.0	4.5866650358524316E-4
9	0.0	0.0	0.0	0.0	5.351109208494504E-4
10	0.0	0.0	0.0	0.0	6.879997553778648E-4
11	0.0	0.0	0.0	0.0	6.879997553778648E-4
12	0.0	0.0	0.0	0.0	9.937774244346935E-4
13	0.0	0.0	0.0	0.0	9.937774244346935E-4
14	0.0	0.0	0.0	0.0	9.937774244346935E-4
15	0.0	0.0	0.0	0.0	0.0010702218416989008
16	0.0	0.0	0.0	1.528888345284144E-4	0.0010702218416989008
17	0.0	0.0	0.0	1.528888345284144E-4	0.0010702218416989008
18	0.0	0.0	0.0	3.057776690568288E-4	0.0012231106762273152
19	0.0	0.0	0.0	3.057776690568288E-4	0.0012231106762273152
20	0.0	0.0	0.0	3.057776690568288E-4	0.0012995550934915222
21	0.0	0.0	0.0	3.057776690568288E-4	0.0012995550934915222
22	0.0	0.0	0.0	5.351109208494504E-4	0.0012995550934915222
23	0.0	0.0	0.0	0.0010702218416989008	0.0014524439280199366
24	0.0	0.0	0.0	0.0018346660143409726	0.0017582215970767655
25	0.0	0.0	0.0	0.0020639992661335943	0.0017582215970767655
26	0.0	0.0	0.0	0.0026755546042472518	0.0018346660143409726
27	0.0	0.0	0.0	0.0036693320286819452	0.0018346660143409726
28	0.0	0.0	0.0	0.0065742198847218184	0.0018346660143409726
29	0.0	0.0	0.0	0.011390218172366871	0.0018346660143409726
30	0.0	0.0	0.0	0.025532435366245203	0.0018346660143409726
31	0.0	0.0	0.0	0.050759093063433576	0.0018346660143409726
32	0.0	0.0	0.0	0.07812619444401975	0.0018346660143409726
33	0.0	0.0	0.0	0.10274129680309448	0.00191111043160518
34	0.0	0.0	0.0	0.13324261929151315	0.001987554848869387
35	0.0	0.0	0.0	0.16382038619719602	0.001987554848869387
36	0.0	0.0	0.0	0.20968703655572032	0.0020639992661335943
37	0.0	0.0	0.0	0.27886923417982784	0.0020639992661335943
38	0.0	0.0	0.0	0.37641231060895625	0.0021404436833978016
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTACG	25	0.0023532247	35.1989	44
CGATTAA	45	0.0013975956	24.446484	33
TTAGGAC	660	0.0	21.999313	3
ATTGTCG	80	3.6169004E-6	21.999313	43
TAGGACG	960	0.0	20.394415	4
GTCGTAA	100	1.1226821E-6	19.801651	30
AATCCCG	345	0.0	19.766743	19
GACGTGA	610	0.0	19.474056	7
GTATCAA	3070	0.0	19.350147	1
CGTCGTA	290	0.0	18.965649	10
GTCCTAC	905	0.0	18.962906	1
TAACGGC	105	1.7921429E-6	18.857994	36
GGACGTG	1010	0.0	18.731373	6
AGGACGT	1020	0.0	18.547731	5
GATATAC	465	0.0	18.453152	1
TTTAGGA	755	0.0	18.357042	2
ATTGCCG	60	0.0074104886	18.334162	28
GTGCGAA	60	0.0074121226	18.333462	11
ATCCCGT	360	0.0	18.33276	20
ATACCGT	340	0.0	18.116386	6
>>END_MODULE