FastQCFastQC Report
Thu 2 Feb 2017
SRR4064013_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064013_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290607
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38240.2962946892431236No Hit
TATCAACGCAGAGTACTTTTTTTTT27100.20997871544164878No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19560.15155659313795755No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17820.13807456491402884No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13500.1046019431166885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATT456.751709E-414.78052215
GCGTTAT602.5706078E-514.2482251
AGTACGG400.005284177614.2476745
CGACCAT1750.013.57237110
ATTTAGA1153.783498E-1013.2157461
CGGTCCA1900.013.00090110
ATTACCG1800.012.6690215
ACCGTCG1207.385097E-1012.6675468
CCGTCAA1750.012.48706418
ATCGCCA2150.012.37439116
TCGCCAG2150.012.37391117
CGCATCG2150.012.37391113
TCCGTCA1850.012.32613717
ACGGTAT1405.2750693E-1112.2151339
ATACCGT1405.2750693E-1112.2137126
GTCTAAA701.0918912E-412.2127661
TTGCGCG1650.012.09221718
TAAGGTG1103.8198777E-812.0889355
ATTACTC550.003077068112.0861233
CGGACAT1501.4551915E-1112.0313695
Produced by FastQC (version 0.11.5)