##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064013_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1290607
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.184712309789116	32.0	32.0	32.0	32.0	32.0
2	30.824940512487537	32.0	32.0	32.0	32.0	32.0
3	30.839777716996732	32.0	32.0	32.0	32.0	32.0
4	30.869205730326893	32.0	32.0	32.0	32.0	32.0
5	30.786177356856115	32.0	32.0	32.0	32.0	32.0
6	34.412228509530784	36.0	36.0	36.0	32.0	36.0
7	34.345536635087214	36.0	36.0	36.0	32.0	36.0
8	34.302831148444106	36.0	36.0	36.0	32.0	36.0
9	34.42670851777497	36.0	36.0	36.0	32.0	36.0
10	34.15505339735489	36.0	36.0	36.0	32.0	36.0
11	34.42068654516828	36.0	36.0	36.0	32.0	36.0
12	34.24331884144438	36.0	36.0	36.0	32.0	36.0
13	34.315908715821315	36.0	36.0	36.0	32.0	36.0
14	34.230032845010136	36.0	36.0	36.0	32.0	36.0
15	34.17635887609474	36.0	36.0	36.0	32.0	36.0
16	34.17221044051365	36.0	36.0	36.0	32.0	36.0
17	34.09616637752623	36.0	36.0	36.0	32.0	36.0
18	34.10350323529936	36.0	36.0	36.0	32.0	36.0
19	34.10859618768533	36.0	36.0	36.0	32.0	36.0
20	34.06642920734197	36.0	36.0	36.0	32.0	36.0
21	34.05067847919622	36.0	36.0	36.0	32.0	36.0
22	34.039998233389404	36.0	36.0	36.0	32.0	36.0
23	33.99456922207922	36.0	36.0	36.0	32.0	36.0
24	33.97395256650553	36.0	36.0	36.0	32.0	36.0
25	33.57935607043817	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	6.0
5	19.0
6	52.0
7	16.0
8	72.0
9	88.0
10	126.0
11	21.0
12	95.0
13	44.0
14	107.0
15	207.0
16	335.0
17	461.0
18	658.0
19	860.0
20	1324.0
21	1962.0
22	3050.0
23	4687.0
24	6999.0
25	10265.0
26	14806.0
27	19527.0
28	26720.0
29	35638.0
30	47906.0
31	64719.0
32	90814.0
33	127492.0
34	268986.0
35	562543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.43153857601726	17.268751627745463	11.29601093844952	26.003698857787754
2	16.101749418655558	19.696747803056542	38.472410450484794	25.729092327803105
3	19.2657145425186	23.834484746487647	28.561244951913388	28.338555759080357
4	12.145504924963273	15.350605314869112	36.44449575690704	36.05939400326058
5	13.954522771160146	37.18829717658071	34.011601776686724	14.845578275572418
6	33.96590336704251	36.05525204386067	16.95749544732458	13.021349141772252
7	29.66191279979044	30.82937694428815	20.729386089320617	18.77932416660079
8	27.661795892551527	33.9407849046308	19.394114145397722	19.003305057419947
9	27.187079929486295	14.10460153089781	18.6284557219515	40.0798628176644
10	15.16688904067002	27.505196264116744	32.24583271634561	25.08208197886762
11	37.11122102181814	21.21795189838876	22.69050557547041	18.980321504322696
12	24.27521989758142	23.763902295308206	29.875446288392432	22.085431518717947
13	29.504866668630804	19.879841276979114	25.55446838797513	25.060823666414944
14	23.061452028836506	20.013506451617904	25.89245701118198	31.03258450836361
15	24.726715374644147	28.03644415225171	22.788914180099606	24.447926293004535
16	24.98575346161808	26.174089210078872	24.662754392779583	24.17740293552346
17	23.288554857186718	26.19505966285506	25.90967343783798	24.606712042120247
18	23.733703874005833	25.879717473075964	27.214268710740225	23.172309942177975
19	25.014906856378545	25.153546436117622	26.00724988853781	23.82429681896602
20	25.224504987922753	25.14339550951253	25.690613089953203	23.941486412611514
21	26.349858179015456	24.593728240287703	25.045632394425173	24.010781186271668
22	25.03373055259175	24.956266593881047	25.797824495670085	24.212178357857127
23	24.179352849583434	24.817926910216563	26.05855185205864	24.944168388141367
24	24.28864530896707	25.438955625238435	25.891170641382327	24.381228424412164
25	24.33225240649673	25.182338554969856	26.061037472647282	24.424371565886137
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	101.0
1	101.0
2	342.0
3	583.0
4	583.0
5	583.0
6	1635.0
7	2687.0
8	2687.0
9	2687.0
10	2764.0
11	2841.0
12	2841.0
13	2841.0
14	3260.0
15	3679.0
16	3679.0
17	3679.0
18	6639.0
19	9599.0
20	9599.0
21	9599.0
22	15882.0
23	22165.0
24	22165.0
25	22165.0
26	32910.5
27	43656.0
28	43656.0
29	43656.0
30	55922.5
31	68189.0
32	68189.0
33	68189.0
34	79376.0
35	90563.0
36	90563.0
37	90563.0
38	100439.5
39	110316.0
40	110316.0
41	110316.0
42	123660.5
43	137005.0
44	137005.0
45	137005.0
46	150417.0
47	163829.0
48	163829.0
49	163829.0
50	166914.5
51	170000.0
52	170000.0
53	170000.0
54	156388.0
55	142776.0
56	142776.0
57	142776.0
58	130396.0
59	118016.0
60	118016.0
61	118016.0
62	102918.0
63	87820.0
64	87820.0
65	87820.0
66	72374.0
67	56928.0
68	56928.0
69	56928.0
70	42884.0
71	28840.0
72	28840.0
73	28840.0
74	21985.5
75	15131.0
76	15131.0
77	15131.0
78	12122.5
79	9114.0
80	9114.0
81	9114.0
82	6189.0
83	3264.0
84	3264.0
85	3264.0
86	2529.0
87	1794.0
88	1794.0
89	1794.0
90	1268.5
91	743.0
92	743.0
93	743.0
94	521.0
95	299.0
96	299.0
97	299.0
98	484.0
99	669.0
100	669.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03711431907621762
2	0.0049589069329393064
3	0.0014721754957163567
4	0.00302183391225989
5	0.008910535895125317
6	0.012164818569866736
7	0.02239256411905406
8	0.0358745923429828
9	0.04997648393352895
10	0.05718239557045638
11	0.05199103987503555
12	0.06214130250339569
13	0.06299361463249463
14	0.0660929314655817
15	0.05756981017459227
16	0.06353599507828486
17	0.06012674656188909
18	0.07438360399408961
19	0.07221408221092866
20	0.07686305746055926
21	0.07384122354829938
22	0.07554584780649726
23	0.07864516463958432
24	0.07399618938995373
25	0.07539088196484289
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1290607.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.66444892182331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94178737960961	39.68045272021379
2	15.22918802755713	16.345319660513653
3	5.1396846640889855	8.274550353908456
4	2.2066987178398323	4.736850825174747
5	1.1101012910572206	2.9786487015995156
6	0.631755497313363	2.034168636999243
7	0.36202892158501726	1.3599657799435354
8	0.2663165703609189	1.1433385589734955
9	0.1689484517636561	0.8159873004082668
>10	0.7878893036672595	7.50637433989823
>50	0.07133437453457236	2.667672431857612
>100	0.07485557787265773	8.11908465845407
>500	0.0075289781998634986	2.7436542835272744
>1k	0.0018822445499658746	1.5939317485281785
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3824	0.2962946892431236	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2710	0.20997871544164878	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1956	0.15155659313795755	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1782	0.13807456491402884	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1350	0.1046019431166885	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5496584165435333E-4
2	0.0	0.0	0.0	0.0	1.5496584165435333E-4
3	0.0	0.0	0.0	0.0	1.5496584165435333E-4
4	0.0	0.0	0.0	7.748292082717666E-5	1.5496584165435333E-4
5	0.0	0.0	0.0	7.748292082717666E-5	2.3244876248153002E-4
6	0.0	0.0	0.0	7.748292082717666E-5	2.3244876248153002E-4
7	0.0	0.0	0.0	7.748292082717666E-5	2.3244876248153002E-4
8	0.0	0.0	0.0	7.748292082717666E-5	2.3244876248153002E-4
9	0.0	0.0	0.0	1.5496584165435333E-4	2.3244876248153002E-4
10	0.0	0.0	0.0	6.198633666174133E-4	2.3244876248153002E-4
11	0.0	0.0	0.0	6.198633666174133E-4	2.3244876248153002E-4
12	0.0	0.0	0.0	6.198633666174133E-4	4.6489752496306003E-4
13	0.0	0.0	0.0	6.198633666174133E-4	5.423804457902367E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATT	45	6.751709E-4	14.780522	15
GCGTTAT	60	2.5706078E-5	14.248225	1
AGTACGG	40	0.0052841776	14.247674	5
CGACCAT	175	0.0	13.572371	10
ATTTAGA	115	3.783498E-10	13.215746	1
CGGTCCA	190	0.0	13.000901	10
ATTACCG	180	0.0	12.66902	15
ACCGTCG	120	7.385097E-10	12.667546	8
CCGTCAA	175	0.0	12.487064	18
ATCGCCA	215	0.0	12.374391	16
TCGCCAG	215	0.0	12.373911	17
CGCATCG	215	0.0	12.373911	13
TCCGTCA	185	0.0	12.326137	17
ACGGTAT	140	5.2750693E-11	12.215133	9
ATACCGT	140	5.2750693E-11	12.213712	6
GTCTAAA	70	1.0918912E-4	12.212766	1
TTGCGCG	165	0.0	12.092217	18
TAAGGTG	110	3.8198777E-8	12.088935	5
ATTACTC	55	0.0030770681	12.086123	3
CGGACAT	150	1.4551915E-11	12.031369	5
>>END_MODULE