FastQCFastQC Report
Thu 2 Feb 2017
SRR4064013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290607
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCG450.00139840124.44403515
ATAACGG500.002579041522.00218835
TAGGACG3250.021.6603374
GATATAC6700.020.3594461
TTAGGAC3500.020.113173
CGGTCCA3950.020.05029910
GTATCAA30700.019.2063791
CCGGTCC4850.018.5976289
GACGTGA2250.018.5767487
ATACACT6750.018.5767484
GCGCTAT600.007413083718.33302711
CGCAATA4400.018.00179136
AGGACGT3600.017.721245
CAATACG4600.017.69672638
CGTCGTA2500.017.59970710
ATACGAA4750.017.59970740
TGGCGAG4000.017.59902418
CGCCGGT3800.017.3674567
AATCCCG3200.017.18654819
CGGGATT2055.456968E-1217.17044444
Produced by FastQC (version 0.11.5)