##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064013_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1290607
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259567009941833	32.0	32.0	32.0	32.0	32.0
2	31.327256864405662	32.0	32.0	32.0	32.0	32.0
3	31.426967310730532	32.0	32.0	32.0	32.0	32.0
4	31.512393005771703	32.0	32.0	32.0	32.0	32.0
5	31.457557567873103	32.0	32.0	32.0	32.0	32.0
6	35.02294269285693	36.0	36.0	36.0	36.0	36.0
7	35.03925672183709	36.0	36.0	36.0	36.0	36.0
8	34.988071504338656	36.0	36.0	36.0	36.0	36.0
9	35.11240214875636	36.0	36.0	36.0	36.0	36.0
10	34.942093913949016	36.0	36.0	36.0	36.0	36.0
11	35.11213560750871	36.0	36.0	36.0	36.0	36.0
12	35.01067017302711	36.0	36.0	36.0	36.0	36.0
13	35.06250624706049	36.0	36.0	36.0	36.0	36.0
14	34.996613221530644	36.0	36.0	36.0	36.0	36.0
15	34.96947637816934	36.0	36.0	36.0	36.0	36.0
16	34.97570445534543	36.0	36.0	36.0	36.0	36.0
17	34.94355214251898	36.0	36.0	36.0	36.0	36.0
18	34.948045377097756	36.0	36.0	36.0	36.0	36.0
19	34.9417832074365	36.0	36.0	36.0	36.0	36.0
20	34.932245059882675	36.0	36.0	36.0	36.0	36.0
21	34.93182665211021	36.0	36.0	36.0	32.0	36.0
22	34.91154239826686	36.0	36.0	36.0	32.0	36.0
23	34.860357955597635	36.0	36.0	36.0	32.0	36.0
24	34.83333423730074	36.0	36.0	36.0	32.0	36.0
25	34.80231937375204	36.0	36.0	36.0	32.0	36.0
26	34.74404524382713	36.0	36.0	36.0	32.0	36.0
27	34.74851445870044	36.0	36.0	36.0	32.0	36.0
28	34.71745697954528	36.0	36.0	36.0	32.0	36.0
29	34.68925862016865	36.0	36.0	36.0	32.0	36.0
30	34.67135541648232	36.0	36.0	36.0	32.0	36.0
31	34.663289444424215	36.0	36.0	36.0	32.0	36.0
32	34.615814884004195	36.0	36.0	36.0	32.0	36.0
33	34.58409182655913	36.0	36.0	36.0	32.0	36.0
34	34.573268237348785	36.0	36.0	36.0	32.0	36.0
35	34.53607643535174	36.0	36.0	36.0	32.0	36.0
36	34.50681655995977	36.0	36.0	36.0	32.0	36.0
37	34.498219829894	36.0	36.0	36.0	32.0	36.0
38	34.45133878864751	36.0	36.0	36.0	32.0	36.0
39	34.44720507482138	36.0	36.0	36.0	32.0	36.0
40	34.423665763474084	36.0	36.0	36.0	32.0	36.0
41	34.411308787260566	36.0	36.0	36.0	32.0	36.0
42	34.327140640024425	36.0	36.0	36.0	32.0	36.0
43	34.32399638309725	36.0	36.0	36.0	32.0	36.0
44	34.25029850295249	36.0	36.0	36.0	32.0	36.0
45	34.21565201490461	36.0	36.0	36.0	32.0	36.0
46	34.184559668435085	36.0	36.0	36.0	32.0	36.0
47	34.14376878476562	36.0	36.0	36.0	32.0	36.0
48	34.085655819316024	36.0	36.0	36.0	32.0	36.0
49	34.062096362409314	36.0	36.0	36.0	32.0	36.0
50	33.45496576417143	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	5.0
20	7.0
21	44.0
22	141.0
23	412.0
24	1108.0
25	2470.0
26	5066.0
27	9227.0
28	15816.0
29	24193.0
30	35004.0
31	49869.0
32	72437.0
33	116524.0
34	236122.0
35	722160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87886518051974	18.05522837730609	11.970436173378298	26.09547026879587
2	15.477428362888016	20.54692306381053	38.060920407685714	25.914728165615735
3	18.366643266806445	24.541494460741696	29.000330077498475	28.091532194953377
4	11.810566655844886	16.17920869792276	36.62935347476032	35.38087117147203
5	13.664113087872606	37.641512869525734	33.732964411319635	14.961409631282024
6	33.804714691725735	36.50608552274749	16.743400764293305	12.94579902123347
7	29.610422414948673	30.94235795205807	20.727442908469918	18.719776724523342
8	27.631571810783605	34.11836445951401	19.174620934180584	19.075442795521795
9	27.272360983584964	13.91105583901874	18.362603005613735	40.45398017178257
10	15.070788999810942	27.722850527973126	32.319873918716624	24.886486553499314
11	37.36528625677685	21.136256040762216	22.571007285719045	18.92745041674189
12	24.287794155135234	23.908669048499036	29.64590970267387	22.157627093691865
13	29.664637642530163	19.828622877350448	25.529674477996352	24.977065002123037
14	23.14476831444429	20.0490931786361	25.891150443163564	30.91498806375605
15	24.768104364077892	28.14191369786271	22.69690885887433	24.393073079185065
16	25.044088021075467	26.21137455447079	24.634588563458856	24.109948860994887
17	23.39302359277456	26.326139560687334	25.790732577771546	24.490104268766558
18	23.952332433238674	25.732350331821124	27.126270237601744	23.18904699733846
19	25.204748776931147	25.081085939473397	25.955636344476936	23.758528939118523
20	25.335968808209543	24.957926415392578	25.770186797417626	23.935917978980253
21	26.487536484770345	24.50862268684425	25.068049375216468	23.935791453168935
22	25.13708665767348	24.90603258776684	25.804137123074643	24.15274363148503
23	24.282675820067485	24.725494829904036	26.048915422731376	24.9429139272971
24	24.382731832952892	25.43727520250177	25.93029940973465	24.249693554810687
25	24.59948240326055	25.10359683243193	26.103767298423964	24.193153465883558
26	24.054547455108146	25.937626399988844	26.462675487739297	23.545150657163713
27	24.420197655839555	25.35485530706084	26.066735579135948	24.158211457963652
28	23.834182839307772	25.567391988246907	26.235244708861465	24.363180463583856
29	23.845144753531958	25.22375719932154	26.12103306050878	24.810064986637716
30	23.78582774785944	25.752741079384762	26.323273023129673	24.13815814962613
31	24.513426914269996	25.349223753491852	25.595251471323245	24.542097860914915
32	24.226774567477268	25.627010742556767	25.573080999993802	24.573133689972167
33	24.05962428363765	25.25919221911932	26.008796263292805	24.67238723395023
34	24.607093737287645	25.446254576448485	26.09325300738043	23.853398678883444
35	24.784282366943135	25.55308637383618	26.11841534322659	23.544215915994098
36	23.958416472234642	25.948170918341653	25.685096833734853	24.408315775688855
37	24.558000925181915	25.787780005067578	25.525180212884823	24.129038856865677
38	24.17520340953119	25.634637737311124	25.827896164277416	24.36226268888028
39	24.63174729612244	25.319746403482533	25.57304847375623	24.47545782663879
40	25.070820871236187	25.69209192766043	25.707278897859876	23.529808303243502
41	23.96146181049526	25.559910240793947	26.687944185642863	23.79068376306793
42	25.017279106383505	26.18311513618725	25.55145919341441	23.248146564014828
43	24.20180165102271	25.308656324142216	26.146100167829694	24.34344185700538
44	23.95352531196851	25.962908580925852	25.410140284132517	24.673425822973126
45	23.936863034067336	26.19482082239842	25.632830230491304	24.23548591304294
46	24.025622330200147	25.592111533225427	25.370740427074995	25.011525709499434
47	24.303451631749496	25.625649902601783	25.787592845996134	24.28330561965259
48	24.864324567248833	25.84132715523253	24.943358799919416	24.35098947759922
49	24.255328710687948	26.151574109156726	25.230144358877205	24.362952821278117
50	24.32331494127118	26.20864982221351	25.050035138885768	24.418000097629537
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	20.5
2	24.0
3	77.5
4	131.0
5	150.5
6	170.0
7	174.5
8	179.0
9	258.5
10	338.0
11	536.0
12	734.0
13	1359.5
14	1985.0
15	2854.5
16	3724.0
17	4231.5
18	4739.0
19	4964.0
20	5189.0
21	5730.5
22	6272.0
23	7195.0
24	8118.0
25	9309.5
26	10501.0
27	13605.0
28	16709.0
29	19379.5
30	22050.0
31	24854.5
32	27659.0
33	31549.5
34	35440.0
35	40561.0
36	45682.0
37	50907.0
38	56132.0
39	58856.5
40	61581.0
41	65712.0
42	69843.0
43	70144.0
44	70445.0
45	75559.0
46	80673.0
47	85518.0
48	90363.0
49	92817.0
50	95271.0
51	92329.0
52	89387.0
53	88132.0
54	86877.0
55	86241.5
56	85606.0
57	82327.5
58	79049.0
59	72100.0
60	65151.0
61	57654.0
62	50157.0
63	43665.5
64	37174.0
65	31557.5
66	25941.0
67	22529.0
68	19117.0
69	16991.5
70	14866.0
71	11575.0
72	8284.0
73	7078.5
74	5873.0
75	4552.5
76	3232.0
77	2742.5
78	2253.0
79	1884.0
80	1515.0
81	1253.5
82	992.0
83	796.0
84	600.0
85	444.0
86	288.0
87	190.5
88	93.0
89	74.0
90	55.0
91	52.5
92	50.0
93	35.0
94	20.0
95	22.5
96	25.0
97	19.0
98	13.0
99	16.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00860060421181661
2	3.8741460413588334E-4
3	7.748292082717666E-5
4	0.0
5	0.0
6	2.3244876248153002E-4
7	3.0993168330870665E-4
8	0.0
9	0.0017046242581978868
10	8.523121290989434E-4
11	0.0
12	3.0993168330870665E-4
13	2.3244876248153002E-4
14	0.0
15	0.0037191801997044803
16	5.423804457902367E-4
17	0.0
18	1.5496584165435333E-4
19	7.748292082717666E-5
20	8.523121290989434E-4
21	0.0
22	0.0
23	9.297950499261201E-4
24	7.748292082717666E-5
25	0.00209203886233377
26	0.004726458170457777
27	0.0062761165870013095
28	0.004106594803840363
29	0.0026344193081240066
30	0.00441652648714907
31	0.00790325792437202
32	0.00302183391225989
33	0.004571492328803424
34	0.0024794534664696532
35	0.0024019705456424767
36	0.0074383603994089605
37	0.003874146041358833
38	0.008290672528507903
39	0.004106594803840363
40	0.00209203886233377
41	0.0033317655955685966
42	0.0024019705456424767
43	0.0010072779707532968
44	9.297950499261201E-4
45	0.0012397267332348266
46	0.0010847608915804734
47	0.0022470047039881234
48	0.00209203886233377
49	3.0993168330870665E-4
50	0.0010847608915804734
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1290607.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.11142716650011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.02756886599764	42.84931534769996
2	14.787525967426026	16.890734245227613
3	4.911844930350606	8.415674219785844
4	2.15027005979082	4.91219967632197
5	1.0198546449029697	2.912267713639639
6	0.5503139913754709	1.8857530462287706
7	0.3549549505441949	1.4190388663775404
8	0.22379962149218358	1.022521262619291
9	0.1609558784728441	0.8273177937379691
>10	0.6842899003400863	6.879390119883974
>50	0.060545133564613246	2.3930823472074545
>100	0.061527680060001554	6.89594685239421
>500	0.005866253215674899	2.2760230429123993
>1k	6.821224669389417E-4	0.4207354659635409
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5496584165435333E-4
2	0.0	0.0	0.0	0.0	1.5496584165435333E-4
3	0.0	0.0	0.0	0.0	1.5496584165435333E-4
4	0.0	0.0	0.0	0.0	1.5496584165435333E-4
5	0.0	0.0	0.0	0.0	1.5496584165435333E-4
6	0.0	0.0	0.0	0.0	1.5496584165435333E-4
7	0.0	0.0	0.0	0.0	1.5496584165435333E-4
8	0.0	0.0	0.0	0.0	1.5496584165435333E-4
9	0.0	0.0	0.0	0.0	1.5496584165435333E-4
10	0.0	0.0	0.0	4.6489752496306003E-4	1.5496584165435333E-4
11	0.0	0.0	0.0	4.6489752496306003E-4	1.5496584165435333E-4
12	0.0	0.0	0.0	4.6489752496306003E-4	4.6489752496306003E-4
13	0.0	0.0	0.0	4.6489752496306003E-4	5.423804457902367E-4
14	0.0	0.0	0.0	4.6489752496306003E-4	5.423804457902367E-4
15	0.0	0.0	0.0	6.198633666174133E-4	7.748292082717667E-4
16	0.0	0.0	0.0	6.198633666174133E-4	7.748292082717667E-4
17	0.0	0.0	0.0	6.198633666174133E-4	7.748292082717667E-4
18	0.0	0.0	0.0	6.198633666174133E-4	7.748292082717667E-4
19	0.0	0.0	0.0	6.198633666174133E-4	7.748292082717667E-4
20	0.0	0.0	0.0	8.523121290989434E-4	8.523121290989434E-4
21	0.0	0.0	0.0	9.297950499261201E-4	8.523121290989434E-4
22	0.0	0.0	0.0	9.297950499261201E-4	8.523121290989434E-4
23	0.0	0.0	0.0	0.0010847608915804734	8.523121290989434E-4
24	0.0	0.0	0.0	0.0018595900998522401	8.523121290989434E-4
25	0.0	0.0	0.0	0.0019370730206794168	8.523121290989434E-4
26	0.0	0.0	0.0	0.0020145559415065935	9.297950499261201E-4
27	0.0	0.0	0.0	0.0023244876248153003	9.297950499261201E-4
28	0.0	0.0	0.0	0.0038741460413588336	9.297950499261201E-4
29	0.0	0.0	0.0	0.007050945795273077	9.297950499261201E-4
30	0.0	0.0	0.0	0.012164818569866738	9.297950499261201E-4
31	0.0	0.0	0.0	0.02533691511048677	0.0010072779707532968
32	7.748292082717666E-5	0.0	0.0	0.04137587972171234	0.0010072779707532968
33	7.748292082717666E-5	0.0	0.0	0.05586518591639438	0.0010072779707532968
34	7.748292082717666E-5	0.0	0.0	0.07360877478581783	0.0011622438124076501
35	7.748292082717666E-5	0.0	0.0	0.09344440251757506	0.0011622438124076501
36	7.748292082717666E-5	0.0	0.0	0.1225779807485935	0.0011622438124076501
37	7.748292082717666E-5	0.0	0.0	0.17177963547385067	0.0011622438124076501
38	7.748292082717666E-5	0.0	0.0	0.23756263525612367	0.0011622438124076501
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCG	45	0.001398401	24.444035	15
ATAACGG	50	0.0025790415	22.002188	35
TAGGACG	325	0.0	21.660337	4
GATATAC	670	0.0	20.359446	1
TTAGGAC	350	0.0	20.11317	3
CGGTCCA	395	0.0	20.050299	10
GTATCAA	3070	0.0	19.206379	1
CCGGTCC	485	0.0	18.597628	9
GACGTGA	225	0.0	18.576748	7
ATACACT	675	0.0	18.576748	4
GCGCTAT	60	0.0074130837	18.333027	11
CGCAATA	440	0.0	18.001791	36
AGGACGT	360	0.0	17.72124	5
CAATACG	460	0.0	17.696726	38
CGTCGTA	250	0.0	17.599707	10
ATACGAA	475	0.0	17.599707	40
TGGCGAG	400	0.0	17.599024	18
CGCCGGT	380	0.0	17.367456	7
AATCCCG	320	0.0	17.186548	19
CGGGATT	205	5.456968E-12	17.170444	44
>>END_MODULE