FastQCFastQC Report
Thu 2 Feb 2017
SRR4064012_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064012_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2269470
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62410.27499812731606943No Hit
TATCAACGCAGAGTACTTTTTTTTT44620.19660978113832744No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG37160.1637386702622198No Hit
GCGCAAGACGGACCAGAGCGAAAGC34720.1529872613429567No Hit
GGGTAGGCACACGCTGAGCCAGTCA34390.1515331773497777No Hit
GAATAGGACCGCGGTTCTATTTTGT33620.14814031469902664No Hit
GATTAAGAGGGACGGCCGGGGGCAT33180.1462015360414546No Hit
GTCTTGCGCCGGTCCAAGAATTTCA32550.14342555750902194No Hit
GTATCTGATCGTCTTCGAACCTCCG30940.13633139014836065No Hit
ATCAGATACCGTCGTAGTTCCGACC30040.13236570653059965No Hit
GAATAACGCCGCCGCATCGCCAGTC29300.129105033333774No Hit
CTATTGGAGCTGGAATTACCGCGGC29210.12870846497199787No Hit
GTCCTATTCCATTATTCCTAGCTGC29020.1278712650971372No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC28980.12769501249190338No Hit
GAACTACGACGGTATCTGATCGTCT28950.12756282303797803No Hit
GTACATGGGGAATAATTGCAATCCC28050.12359713942021705No Hit
TCGTAGTTCCGACCATAAACGATGC27790.12245149748619721No Hit
GTGCATGGCCGTTCTTAGTTGGTGG27000.11897050853282926No Hit
CTCTTAATCATGGCCTCAGTTCCGA25480.11227290953394403No Hit
GGTATCAACGCAGAGTACTTTTTTT25030.11029006772506356No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24780.10918848894235218No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG23860.1051346790219743No Hit
CTTTAATATACGCTATTGGAGCTGG23810.10491436326543202No Hit
GTATCAACGCAGAGTACATGGGGAA23750.1046499843575813No Hit
GCTTTGAACACTCTAATTTTTTCAA22980.10125712170683022No Hit
CATCTAAGGGCATCACAGACCTGTT22900.10090461649636259No Hit
GTCCTGTATTGTTATTTTTCGTCAC22800.10046398498327805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA2800.013.5736516
CGTTATT2350.013.3329042
GCGTTAT2450.013.1779831
TAACCCG655.460941E-513.1522285
CGCCAGT5100.013.043076518
GCTCGTA2350.012.9374319
CGCATCG5300.012.72737513
CGGTCCA5600.012.55424110
ATCGCCA5500.012.26564416
TGCGTAC1405.2750693E-1112.21628516
CGGACAT4300.012.1496685
TATGCCG550.00307202212.0894225
GATAACG3150.012.0638711
TAATATA5800.011.9543714
GTTCTAG802.8804187E-511.8698741
TACGCTA5250.011.7630529
TCGCCAG5500.011.74789517
ACGGTAT5500.011.7465999
TTAATAT5500.011.7411613
TTTTCGG4300.011.7112216
Produced by FastQC (version 0.11.5)