##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064012_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2269470
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221738115066515	32.0	32.0	32.0	32.0	32.0
2	30.75540765024433	32.0	32.0	32.0	32.0	32.0
3	30.783175807567407	32.0	32.0	32.0	32.0	32.0
4	30.82699617091215	32.0	32.0	32.0	32.0	32.0
5	30.69203602603251	32.0	32.0	32.0	32.0	32.0
6	34.3645428227736	36.0	36.0	36.0	32.0	36.0
7	34.28564202214614	36.0	36.0	36.0	32.0	36.0
8	34.23303282264141	36.0	36.0	36.0	32.0	36.0
9	34.414938289556595	36.0	36.0	36.0	32.0	36.0
10	34.03794938906441	36.0	36.0	36.0	32.0	36.0
11	34.39528127712638	36.0	36.0	36.0	32.0	36.0
12	34.15390245299563	36.0	36.0	36.0	32.0	36.0
13	34.281505373501304	36.0	36.0	36.0	32.0	36.0
14	34.15450171185343	36.0	36.0	36.0	32.0	36.0
15	34.07580977056317	36.0	36.0	36.0	32.0	36.0
16	34.07163302445064	36.0	36.0	36.0	32.0	36.0
17	33.975462552930864	36.0	36.0	36.0	32.0	36.0
18	33.98188651976012	36.0	36.0	36.0	32.0	36.0
19	33.99811233459795	36.0	36.0	36.0	32.0	36.0
20	33.98833295879655	36.0	36.0	36.0	32.0	36.0
21	33.963362370950044	36.0	36.0	36.0	32.0	36.0
22	33.94121182478729	36.0	36.0	36.0	32.0	36.0
23	33.88231216980176	36.0	36.0	36.0	32.0	36.0
24	33.859743904964596	36.0	36.0	36.0	32.0	36.0
25	33.45152700850859	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	19.0
5	23.0
6	106.0
7	33.0
8	124.0
9	159.0
10	199.0
11	56.0
12	136.0
13	125.0
14	225.0
15	398.0
16	657.0
17	838.0
18	1141.0
19	1628.0
20	2445.0
21	3707.0
22	5736.0
23	8932.0
24	13575.0
25	19536.0
26	27895.0
27	36340.0
28	49425.0
29	66390.0
30	86499.0
31	117392.0
32	165444.0
33	229667.0
34	478410.0
35	952205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.64637344355906	17.233950921224373	11.377364553766999	25.742311081449575
2	17.164400089188376	19.454919770119602	36.7421742046415	26.63850593605052
3	18.590447200595392	23.564561834717015	28.55947465199396	29.285516312693634
4	12.368097640464143	15.128490645271167	35.79534914053883	36.70806257372586
5	14.839631400983851	36.43094761640566	33.19784015889013	15.531580823720354
6	34.78627408812393	35.58260120008743	16.416457485739265	13.21466722604937
7	30.741152582771065	30.240874699584253	20.542024267730426	18.475948449914256
8	28.873150657397105	32.049453329830506	19.013648681767613	20.063747331004777
9	27.404428581757006	13.661656283228913	18.54179855366618	40.392116581347906
10	16.37185933193072	26.02021561203237	30.586231586298602	27.021693469738306
11	37.98580200757603	20.80677786164232	22.034751662986814	19.17266846779484
12	24.971803375834767	23.688779815890815	28.09516922824583	23.244247580028592
13	29.378828256034822	18.823511775111506	25.49638360426172	26.301276364591953
14	24.07138192690813	19.62368044982325	24.512889162855895	31.792048460412726
15	25.454908595017926	27.028477227344734	21.927495918377243	25.58911825926009
16	26.377101455409992	25.362265162578396	23.23924844970264	25.021384932308973
17	24.188990598353534	25.783782854285725	24.621705312197008	25.405521235163736
18	25.42645545204146	24.325710450396578	25.812009458266417	24.43582463929555
19	25.871796138168	24.466030377487286	25.067600580668266	24.594572903676447
20	26.054374999724384	23.744555422131455	24.38725484791784	25.81381473022632
21	27.864018460596828	23.57566908859843	23.889416333112703	24.67089611769204
22	26.54875569037983	23.576153742013183	24.68845404677897	25.186636520828014
23	24.733045471243706	23.458481751657427	25.42688689542456	26.3815858816743
24	25.179324717750433	24.740108146056265	24.84678132747476	25.23378580871854
25	25.124060314502213	24.115481818819244	24.976241893028327	25.78421597365022
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	140.0
1	140.0
2	454.0
3	768.0
4	768.0
5	768.0
6	2300.5
7	3833.0
8	3833.0
9	3833.0
10	4063.5
11	4294.0
12	4294.0
13	4294.0
14	4856.5
15	5419.0
16	5419.0
17	5419.0
18	9551.5
19	13684.0
20	13684.0
21	13684.0
22	21773.5
23	29863.0
24	29863.0
25	29863.0
26	45874.5
27	61886.0
28	61886.0
29	61886.0
30	76123.0
31	90360.0
32	90360.0
33	90360.0
34	112885.0
35	135410.0
36	135410.0
37	135410.0
38	154975.5
39	174541.0
40	174541.0
41	174541.0
42	201127.5
43	227714.0
44	227714.0
45	227714.0
46	256894.5
47	286075.0
48	286075.0
49	286075.0
50	295905.5
51	305736.0
52	305736.0
53	305736.0
54	284044.0
55	262352.0
56	262352.0
57	262352.0
58	245247.5
59	228143.0
60	228143.0
61	228143.0
62	205298.0
63	182453.0
64	182453.0
65	182453.0
66	152216.0
67	121979.0
68	121979.0
69	121979.0
70	91727.0
71	61475.0
72	61475.0
73	61475.0
74	48362.5
75	35250.0
76	35250.0
77	35250.0
78	28518.0
79	21786.0
80	21786.0
81	21786.0
82	14788.5
83	7791.0
84	7791.0
85	7791.0
86	6119.0
87	4447.0
88	4447.0
89	4447.0
90	3169.0
91	1891.0
92	1891.0
93	1891.0
94	1347.5
95	804.0
96	804.0
97	804.0
98	1090.0
99	1376.0
100	1376.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040229657144619665
2	0.005111325551780812
3	0.0018065892036466663
4	0.003657241558601788
5	0.010090461649636258
6	0.013307071695153496
7	0.023397533344789754
8	0.03639616298078406
9	0.04908635055761918
10	0.05825148602977788
11	0.05560769695127057
12	0.06468470612081235
13	0.06459657981819544
14	0.06860632658726487
15	0.06067495935174292
16	0.0668878636862351
17	0.06446439036427007
18	0.08037118798662243
19	0.07658175697409528
20	0.08138464046671691
21	0.07583268340185154
22	0.0805033774405478
23	0.08587908190017934
24	0.07913741974998569
25	0.08032712483531397
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2269470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.344976316930946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.76780081989055	26.752446701230586
2	15.254680848790603	11.698807517384315
3	5.967949437229119	6.865226394935757
4	2.875487453662725	4.410419932413171
5	1.5763214190321384	3.0222003740329164
6	0.9939922942802459	2.286876659003273
7	0.6800024023209321	1.8252273208716605
8	0.4728214563221017	1.4504262035846232
9	0.3553835501657815	1.2264456433080357
>10	1.776009606471301	12.23557314641232
>50	0.12723853421597098	3.4068118205358204
>100	0.11712152341739987	9.7116048333343
>500	0.022037426073549592	5.961493570646543
>1k	0.01303784893356599	8.870324782346017
>5k	1.1537919410235388E-4	0.2761150999607047
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6241	0.27499812731606943	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4462	0.19660978113832744	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3716	0.1637386702622198	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3472	0.1529872613429567	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	3439	0.1515331773497777	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3362	0.14814031469902664	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3318	0.1462015360414546	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	3255	0.14342555750902194	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3094	0.13633139014836065	No Hit
ATCAGATACCGTCGTAGTTCCGACC	3004	0.13236570653059965	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2930	0.129105033333774	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2921	0.12870846497199787	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2902	0.1278712650971372	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2898	0.12769501249190338	No Hit
GAACTACGACGGTATCTGATCGTCT	2895	0.12756282303797803	No Hit
GTACATGGGGAATAATTGCAATCCC	2805	0.12359713942021705	No Hit
TCGTAGTTCCGACCATAAACGATGC	2779	0.12245149748619721	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	2700	0.11897050853282926	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	2548	0.11227290953394403	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2503	0.11029006772506356	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2478	0.10918848894235218	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	2386	0.1051346790219743	No Hit
CTTTAATATACGCTATTGGAGCTGG	2381	0.10491436326543202	No Hit
GTATCAACGCAGAGTACATGGGGAA	2375	0.1046499843575813	No Hit
GCTTTGAACACTCTAATTTTTTCAA	2298	0.10125712170683022	No Hit
CATCTAAGGGCATCACAGACCTGTT	2290	0.10090461649636259	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	2280	0.10046398498327805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3218945392536583E-4
2	0.0	0.0	0.0	0.0	1.3218945392536583E-4
3	0.0	0.0	0.0	0.0	1.3218945392536583E-4
4	0.0	0.0	0.0	0.0	1.3218945392536583E-4
5	0.0	0.0	0.0	0.0	2.6437890785073167E-4
6	1.3218945392536583E-4	0.0	0.0	0.0	3.525052104676422E-4
7	1.3218945392536583E-4	0.0	0.0	0.0	3.525052104676422E-4
8	1.3218945392536583E-4	0.0	0.0	4.4063151308455275E-5	3.965683617760975E-4
9	1.3218945392536583E-4	0.0	0.0	4.4063151308455275E-5	3.965683617760975E-4
10	1.3218945392536583E-4	0.0	0.0	4.4063151308455275E-5	4.8469466439300804E-4
11	1.3218945392536583E-4	0.0	0.0	8.812630261691055E-5	5.287578157014633E-4
12	1.3218945392536583E-4	0.0	0.0	8.812630261691055E-5	0.0011456419340198371
13	1.3218945392536583E-4	0.0	0.0	8.812630261691055E-5	0.0017184629010297558
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	280	0.0	13.57365	16
CGTTATT	235	0.0	13.332904	2
GCGTTAT	245	0.0	13.177983	1
TAACCCG	65	5.460941E-5	13.152228	5
CGCCAGT	510	0.0	13.0430765	18
GCTCGTA	235	0.0	12.937431	9
CGCATCG	530	0.0	12.727375	13
CGGTCCA	560	0.0	12.554241	10
ATCGCCA	550	0.0	12.265644	16
TGCGTAC	140	5.2750693E-11	12.216285	16
CGGACAT	430	0.0	12.149668	5
TATGCCG	55	0.003072022	12.089422	5
GATAACG	315	0.0	12.06387	11
TAATATA	580	0.0	11.954371	4
GTTCTAG	80	2.8804187E-5	11.869874	1
TACGCTA	525	0.0	11.763052	9
TCGCCAG	550	0.0	11.747895	17
ACGGTAT	550	0.0	11.746599	9
TTAATAT	550	0.0	11.741161	3
TTTTCGG	430	0.0	11.71122	16
>>END_MODULE