FastQCFastQC Report
Thu 2 Feb 2017
SRR4064012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2269470
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA33180.1462015360414546No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT33130.14598122028491234No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA33110.1458930939822954No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC33060.14567277822575314No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC32860.14479151519958405No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT32750.14430682053519103No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG29920.1318369487148982No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT28890.1272984441301273No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA28760.1267256231631174No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC28500.12557998122909755No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG28170.12412589723591852No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA27990.12333276051236632No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA26430.11645890890824728No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT24490.10791065755440697No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA24490.10791065755440697No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT22740.1001996060754273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC450.0013993324.442236
GTATCAA47950.022.4816951
GATATAC3750.018.7732851
ACCGTAT851.4325269E-418.1160058
AACGCAG60200.017.7591886
ATACCGT9050.017.7442476
ACGATAC750.001292128617.5984043
CGTCGTA8450.017.4425910
TACCGTC9000.017.109567
ATAGTAC2600.016.9215433
CGTCTTA2900.016.68887715
TCTAGAT7650.016.6782282
GGTATCA24450.016.6461751
TACGCTC1206.407641E-616.50104934
CCGTCGT8700.016.4356569
GTCCTAT9250.016.410771
GTCGTAG8800.016.24888611
TATACTG3250.016.244685
TAACGGC2850.016.21298636
ACCGTCG9000.016.131878
Produced by FastQC (version 0.11.5)